Abstract：
:In this study, we investigated the role of AMP-activated protein kinase (AMPK) and the importance of reactive oxygen species (ROS) in apoptosis induction associated with cell cycle arrest induced by sulforaphane in AGS human gastric cancer cells. Our results demonstrated that sulforaphane inhibited proliferation of AGS cells by promoting apoptosis and accumulating the cellular portion of the G2/M phase via the buildup of cyclin B1 and cyclin-dependent kinase p21 (WAF1/CIP1). Moreover, the phosphorylation of histone H3 was markedly increased following treatment with sulforaphane, indicating that sulforaphane stimulated mitotic arrest. Sulforaphane concurrently induced phosphorylation of AMPK; however, compound C, an AMPK inhibitor, significantly blocked sulforaphane-induced apoptosis, suggesting that sulforaphane induces apoptosis of AGS cells through the AMPK-dependent pathway. Sulforaphane also activated the mitochondrial apoptotic signaling pathway with a decrease in mitochondrial membrane potential and the nuclear translocation of cytochrome c. Furthermore, sulforaphane provoked the generation of intracellular ROS; especially when ROS production was blocked by antioxidant N-acetylcysteine, both AMPK activation and growth inhibition by sulforaphane were completely abolished. Collectively, these findings suggest that sulforaphane inhibited growth of AGS cells, which was mediated by a complex interplay between cellular mechanisms governing redox homeostasis, apoptosis, and cell cycle arrest through an ROS/AMPK-dependent pathway.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Choi YHdoi
10.4149/gpb_2017026subject
Has Abstractpub_date
2018-03-01 00:00:00pages
129-140issue
2eissn
0231-5882issn
1338-4325journal_volume
37pub_type
杂志文章abstract：:Homeostasis and development in vertebrates are regulated by cell proliferation, differentiation and death. Permeability of mitochondrial membranes, a decisive feature of apoptosis, is regulated by Bcl-2 family regulators. Protein p53 is able to reduce bcl-2 and promote bax expression. This study focused on the immunoh...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:The fibroblast growth factor receptors (FGFRs) and Ras/mitogen activated protein (RAS/MAP) signalling cascades are the main molecular pathways involved in breast carcinogenesis. This study aims to determine the association between FGF10 (rs4415084 C>T), FGFR2 (rs2981582 C>T) and MAP3K1 (rs889312 A>C) gene polymorphism...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017033
更新日期：2017-12-01 00:00:00
abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Cytochrome P-450 and NADPH: cytochrome P-450 reductase were solubilized by detergents from microsomal fraction of tulip bulbs Tulipa fosteriana L. and purified to electrophoretic homogeneity. The purification was achieved by anion-exchange column chromatography, hydroxyapatite-column chromatography and affinity chroma...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Hyperbaric oxygen treatment (HBO) could transiently reverse hypoxia during acute myocardial infarction (AMI). In order to evaluate whether early HBO can identify viable segments after AMI, improvement of wall motion score index (WMSI) after HBO was compared to dobutamine stress echocardiography (DSE). Thirty-one patie...
journal_title：General physiology and biophysics
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Cochlear spiral ganglion neurons provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Serotonin plays a role in the response properties of central auditory neurons. However, knowledge about the role of serotonin in the peripheral auditory nervous system re...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013005
更新日期：2013-03-01 00:00:00
abstract：:Effects of haloperidol on L-type CaV1.2 channel were studied. Calcium current was measured in whole cell patch-clamp using calcium as a charge carrier. Inhibition by haloperidol was investigated in CaV1.2 channel natively expressed in rat cardiac myocytes and recombinant cardiac (CaV1.2a) and vascular (CaV1.2b) splice...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_03_249
更新日期：2009-09-01 00:00:00
abstract：:Thermal inactivation of peroxidase (POD) in an extract of Picea omorika (Pancić) Purkyne needles initiated by heat treatment was studied. This is the first study of this kind on a conifer species. Non-linear regression analysis was applied on the inactivation rate data, combining Mitscherlich and Arrhenius equations, ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_01_78
更新日期：2009-03-01 00:00:00
abstract：:The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb-2019057
更新日期：2020-03-01 00:00:00
abstract：:The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptotheci...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018017
更新日期：2018-09-01 00:00:00
abstract：:A possible mechanism is put forward to explain the sliding of thin filaments during muscle contraction. In our model, repulsion due to electrostatic forces is the mechanism which triggers crossbridges to cause the thin filaments to slide. The mechanism proposed could operate regardless of whether the myosin heads rota...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats recei...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:Aquaporin attracted attention not only of physiologists and biophysicists, but also of chemical engineers. Here we critically analyze a paper describing aquaporin-based artificial membranes, suggested for forward osmosis-based water purification (Wang et al. 2012, Small 8, pp. 1185-1190). Related papers published late...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013058
更新日期：2013-12-01 00:00:00
abstract：:Beat-to-beat variability of action potential duration (short-term variability, SV) is an intrinsic property of mammalian myocardium. Since the majority of agents and interventions affecting SV may modify also action potential duration (APD), we propose here the concept of relative SV (RSV), where changes in SV are nor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015019
更新日期：2016-01-01 00:00:00
abstract：:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:The purpose of this study was to evaluate the effect of resveratrol on cytogenetic damages of iododeoxyuridine (IUdR) and x-ray megavoltage radiation (6 MV) in spheroid model of U87MG glioblastoma cancer cell line using clonogenic and alkaline comet assay. Cells were cultured as spheroids (350 µm) that were treated wi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014023
更新日期：2015-01-01 00:00:00
abstract：:Elevated serum resistin is implicated in insulin resistance associated with obesity and type 2 diabetes mellitus. Alcohol consumption interferes with the nutritional status, metabolic and hormonal activity of the drinker. Impact of ethanol intake on resistin level and resistin metabolic effects is unknown. Effect of l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:The substantia gelatinosa of the trigeminal subnucleus caudalis has been considered to be an essential location for the transference of orofacial sensory signals. The co-localization of inhibitory and excitatory neurotransmitters in the same substantia gelatinosa (SG) neurons has demonstrated their essential part in t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019027
更新日期：2019-09-01 00:00:00
abstract：:Fasting for 48 h and the same period of recovery induced by 48 h refeeding increased rat hypothalamic monoamine oxidase (MAO) activity. However, in the interscapular brown adipose tissue (IBAT), only refeeding induced a significant elevation of the enzyme activity. As far as hypothalamic antioxidative enzymes are conc...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:The purpose of this study was to assess the possible protective effects of Artemisia campestris against fenthion-induced nephrotoxicity in adult rats and their progeny. Fenthion was administered orally at a dose of 551 ppm, which represented ¼ of LD50, for 21 consecutive days to pregnant and lactating rats. Oxidative ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013047
更新日期：2013-12-01 00:00:00
abstract：:The effects of different parts of extract from medicinal plant Conyza canadensis, used to control bleeding, on human blood platelet aggregation in vitro were investigated. Aqueous extract of Conyza c. from young or old plants, glycoconjugate part, polysaccharide part and aglycon part at the concentrations above 0.75 m...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:Low-conductance chloride channel from skeletal muscle SR vesicles of the crayfish Astacus fluviatilis was incorporated into planar lipid bilayers and its basic characteristics were investigated. The channel has a relatively low unitary conductance of 26 pS in symmetrical 160 mmol/l choline-chloride. The dependence of ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted from intestinal L cells upon nutrients ingestion, and is currently used for treating diabetes mellitus. It plays an important role in receptor modulation and cross talk with insulin at the coronary endothelium (CE) and cardiomyocytes (CM) in diabetic type...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_01_34
更新日期：2011-03-01 00:00:00
abstract：:Hyperglycemia impairs oxidative capacity in skeletal muscle. Muscle oxidative capacity is regulated by peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α). Transcutaneous carbon dioxide (CO2) enhances PGC-1α expression in skeletal muscle. Therefore, the aim of this study was to clarify the effects of...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018048
更新日期：2019-05-01 00:00:00
abstract：:Calcium ions mediate extracellular signals on intracellular processes. The signalling system based on transient rises or oscillations of the cytoplasmic calcium concentration has potential advantages. The relevant mechanisms of intracellular concentration changes include calcium-induced calcium release and calcium dep...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:Homocysteine (Hcys) may be implicated in different diseases, especially in cardiovascular illnesses. The most reactive form of Hcys is its cyclic thioester-homocysteine thiolactone (HTL), which is formed in plasma and represents up to 0.29% of plasma total Hcys. Recently, it has been observed that Hcys and HTL may mod...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_02_186
更新日期：2010-06-01 00:00:00
abstract：:Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020022
更新日期：2020-09-01 00:00:00
abstract：:Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, gui...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013016
更新日期：2013-06-01 00:00:00
abstract：:In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016039
更新日期：2016-10-01 00:00:00
