Abstract:
:Cytochrome P-450 and NADPH: cytochrome P-450 reductase were solubilized by detergents from microsomal fraction of tulip bulbs Tulipa fosteriana L. and purified to electrophoretic homogeneity. The purification was achieved by anion-exchange column chromatography, hydroxyapatite-column chromatography and affinity chromatography. The two enzyme showed relative molecular weights of about 54,200 and 77,600 for cytochrome P-450 and NADPH: cytochrome P-450 reductase, respectively. The purified enzymes were characterized by their absorption spectra and by kinetic characteristics. The interaction with endogeneous as well as exogenous substrates was studied by differential spectroscopy. Both enzymes in the presence of dilauroyl phosphatidylcholine and NADPH were able to oxidize xenobiotics (N-nitroso-N-methylaniline and N-nitroso-N-dimethylamine) in the reconstitution experiments.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Hansíková H,Frei E,Anzenbacher P,Stiborová Msubject
Has Abstractpub_date
1994-04-01 00:00:00pages
149-69issue
2eissn
0231-5882issn
1338-4325journal_volume
13pub_type
杂志文章abstract::Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, gui...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013016
更新日期:2013-06-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017026
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:1991-06-01 00:00:00
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journal_title:General physiology and biophysics
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doi:10.4149/gpb_2014020
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:1997-03-01 00:00:00
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更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:General physiology and biophysics
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doi:10.4149/gpb_2016039
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pub_type: 杂志文章
doi:10.4149/gpb_2016057
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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journal_title:General physiology and biophysics
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doi:
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
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更新日期:1998-12-01 00:00:00
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journal_title:General physiology and biophysics
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pub_type: 杂志文章
doi:10.4149/gpb_2016001
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
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doi:
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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