Abstract:
:Low-conductance chloride channel from skeletal muscle SR vesicles of the crayfish Astacus fluviatilis was incorporated into planar lipid bilayers and its basic characteristics were investigated. The channel has a relatively low unitary conductance of 26 pS in symmetrical 160 mmol/l choline-chloride. The dependence of the channel conductance on Cl- concentration shows saturating behavior with a maximum conductance of 37 pS and an ionic activity for half-maximum conductance Km = 75 mmol/l. The channel exhibits a complex kinetics with several modes of activity. Open state probability slightly decreases with the increasing absolute value of voltage. The channel activity does not appear to be dependent on the presence of Ca2+ ions. The channel is effectively inhibited by DIDS, a stilbene derivative. The permeability properties of the channel are similar to the specific behavior of the "double-barrelled" channel from Torpedo electroplax described by Miller and White (1984).
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Proks P,Hurnák O,Zachar Jsubject
Has Abstractpub_date
1991-12-01 00:00:00pages
537-48issue
6eissn
0231-5882issn
1338-4325journal_volume
10pub_type
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015035
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016035
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:General physiology and biophysics
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pub_type: 杂志文章
doi:
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journal_title:General physiology and biophysics
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更新日期:2000-09-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章,评审
doi:
更新日期:2009-01-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2006-12-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2010_02_186
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2009_04_371
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:1988-06-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
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更新日期:1985-06-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
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journal_title:General physiology and biophysics
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doi:
更新日期:2008-09-01 00:00:00
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journal_title:General physiology and biophysics
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