Abstract：
:The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptothecin (CPT-C). In-vitro method revealed significant reduction in bound fraction of drugs to HSA oxidized by chloramine T. Oxidative stress was determined by measuring the plasma level of advanced oxidation protein products (AOPP). A significant positive correlation between the AOPP and the plasma free fraction of tested drugs in thirty healthy patients was also found. The oxidative stress monitoring by AOPP is very important for improvement of dosage of drugs highly bound to albumin and with narrow therapeutic index. As a result of severe oxidative stress, the drug pharmacokinetics and therapeutic effects are prone to change. This study highlights the issues of therapeutic drug monitoring and it can explain the behaviour of drugs in pathological situations.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Wybranowski T,Ziomkowska B,Cyrankiewicz M,Kruszewski Sdoi
10.4149/gpb_2018017subject
Has Abstractpub_date
2018-09-01 00:00:00pages
647-655issue
6eissn
0231-5882issn
1338-4325journal_volume
37pub_type
杂志文章abstract：:The role of C-type natriuretic peptide (CNP) in the gastrointestinal tract is still unclear. This study was designed to investigate the effect of CNP on barium current (I(Ba)) through the L-type calcium channel in gastric antral myocytes of guinea pigs. The whole-cell patch clamp technique was performed in gastric ant...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
abstract：:The character of structural changes in thylakoid membranes caused by temperature variation was investigated. Experiments were performed on maize leaf segments in vivo and in a closed temperature cycle 24-50-24 degrees C. Two biophysical methods were used for detection: luminescence and EPR. Arrhenius plots of delayed ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:Electron spin resonance spectroscopy (ESR) was used to study the effects of amphiphilic amines of the carbamate, amide, and ester type and amine oxide on the photosynthetic system of spinach chloroplasts. The ESR signal II connected to the photosynthetic center PS II donor side was observed to diminish in the presence...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Aiming at making the Münch model more adequate to the biological reality we introduce certain modifications and complements. Considering the model within the framework of so-called integrated system of long-distance water transport in plants we present a quantitative analysis based on the Kedem-Katchalsky formalism. A...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bone marrow phagocytes were studied using luminol- and lucigenin-amplified chemiluminescence (CL). NDGA applied in vitro strongly suppressed the opsonized zymosan particles stimulated CL response in a dose-dependent manner (in concentrations of 0.3-30...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The ionic composition of intracellular space is rigorously maintained in the expense of high-energy expenditure. It has been recently postulated that the cytoplasmic ionic composition is optimized so the energy cost of the fluctuations of calcium ion concentration is minimized. Specifically, thermodynamic arguments ha...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016029
更新日期：2017-04-01 00:00:00
abstract：:CRM197, cross-reacting material 197, is a mutant of diphtheria toxin (DTx). CRM197 is used in pharmacology as a carrier protein. It has been recently shown that CRM197 causes breakdown in actin filaments. In order to show intracellular localization of CRM197 and visualize cell structure via actin cytoskeleton, endothe...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017006
更新日期：2017-10-01 00:00:00
abstract：:The upregulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) was reported to be involved in regulating the levels of inflammatory markers and apoptosis in macrophages. This study aims to investigate the function and regulation of PCSK9 in myocardial ischaemia. The results of our study showed dramatically ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb-2019057
更新日期：2020-03-01 00:00:00
abstract：:The voltage dependent ionic currents in cultured embryonic skeletal myocytes at stages of development ranging from 1 to 6 day were studied using the whole-cell patch clamp technique. Sodium (INa) and calcium (ICa) inward and potassium (IK) outward currents were observed at all stages. INa did not differ from that desc...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Voltage-activated calcium channels can be divided into two subgroups based on their activation threshold, low-voltage-activated (LVA) and high-voltage-activated (HVA). Auxiliary subunits of the HVA calcium channels contribute significantly to biophysical properties of the channels. We have cloned and characterized mem...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2005-06-01 00:00:00
abstract：:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:Semiserial sections of lung capillaries of seven rabbits were examined for megakaryocytes. Triads of serial sections of each lobe were examined, and megakaryocyte counts per cm2 were determined. Median megakaryocyte count was determined for each lobe. The lobal values were used to calculate the was determined for each...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:A possible mechanism is put forward to explain the sliding of thin filaments during muscle contraction. In our model, repulsion due to electrostatic forces is the mechanism which triggers crossbridges to cause the thin filaments to slide. The mechanism proposed could operate regardless of whether the myosin heads rota...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Currents through DPI 201-106 modified single cardiac sodium channels in guinea pig ventricular cells were measured using the patch clamp technique in the cell-free configuration to control the sodium concentrations on both sides of the patch membrane. Current-voltage relationships of the single channels were obtained ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:Cytochrome P-450 and NADPH: cytochrome P-450 reductase were solubilized by detergents from microsomal fraction of tulip bulbs Tulipa fosteriana L. and purified to electrophoretic homogeneity. The purification was achieved by anion-exchange column chromatography, hydroxyapatite-column chromatography and affinity chroma...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Stationary and time-dependent fluorescence were measured for a donor/ acceptor (DA) pair bound to membrane proteins of bovine erythrocyte ghosts. The donor N-(p-(2-benzoxazolyl)phenyl)-maleimide (BMI) and the acceptor fluram bind to SH- and NH2-residues, respectively. The fluorescence spectra and the time-dependent em...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020022
更新日期：2020-09-01 00:00:00
abstract：:In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016039
更新日期：2016-10-01 00:00:00
abstract：:The present results suggest that laminin-1 and 3 are localized in the specialized Schwann cells of Pacinian corpuscles, in spite of incomplete deposition of the basal lamina on the surface of their cytoplasmic processes. In addition, laminin-3 is concentrated and probably function as a stop protein not only in the neu...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Although the A1 adenosine receptor (A1 receptor), the main adenosine receptor type in cardiac muscle, is involved in powerful cardioprotective processes such as ischemic preconditioning, the atrial A1 receptor reserve has not yet been quantified for the direct negative inotropic effect of adenosine. In the present stu...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013041
更新日期：2013-09-01 00:00:00
abstract：:A mechanical model of heart muscle is proposed which includes rheological equations and equations for Ca-troponin interaction, for the dependences of the number of myosin cross-bridges on the length of sarcomere and on the speed of motion. The main assumption of the model is the dependence of the troponin affinity to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:The passive mechanical properties of whole muscle in active and nonactive states are compared. The experimental results are presented as stress-strain curves, which are analyzed in the framework of the current theoretical background [viz. the freely-jointed chain model (FJCM) and the worm-like chain model (WLCM)] in a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:It is currently believed that the protein folding rate is related to the protein structures and its amino acid sequence. However, few studies have been done on the problem that whether the protein folding rate is influenced by its corresponding mRNA sequence. In this paper, we analyzed the possible relationship betwee...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_02_154
更新日期：2011-06-01 00:00:00
abstract：:The effects of thrombin stimulation on megakaryocytopoiesis and pulmonary-platelet interactions were investigated before and after administration of the compound to 15 mongrel dogs. Each dog served as its own control. Thrombin was given to encourage the traffic of megakaryocytes into the lung and to study the thrombin...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The dynamics of a bioreactor with a variable volume and an active solute flux based on the thermodynamics of irreversible processes and stability analysis was studied. The active solute flux may control both the bioreactor volume and the hydrostatic pressure as well as the concentration of the solute inside the cell i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:The effect of binding of an antitumour drug cis-diamminedichloroplatinum(II) (cis-[Pt(NH3)2Cl2]) to DNA on cutting effectiveness of BamHI, EcoRI, and SalI restriction endonucleases was quantitatively determined. The platinum complex inhibits the cleavage of plasmid pHC624 DNA linearized by BglI restrictase. From the p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Arterial base deficit/excess (BD/E) is commonly used marker of metabolic acidosis in critically ill patients, but requires an arterial puncture and blood gas analysis. We hypothesized that serum bicarbonate (HCO3), which can be routinely obtained, strongly correlates with arterial BD/E and provides equivalent predicti...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:The role of vascular endothelial growth factor (VEGF) in chronic stress and neurodevelopmental disorders is of growing research interest. Here we show that post-weaning isolation rearing of rats decreased gene expression of VEGF in the hippocampus. Gene expression of VEGF upstream regulator fibroblast growth factor-2 ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019019
更新日期：2019-07-01 00:00:00
abstract：:The effect of K+ and Na+ on the properties of DNA aptamers that selective binds to fibrinogen (FIBRI) and heparin (HEPA) exosites of human alpha-thrombin was studied by circular dichroism (CD). The complexes of FIBRI-K+ were slightly more stable than HEPA-K+. However, lower stability was observed for HEPA-K+ at presen...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:Liposomes and proteoliposomes obtained from rat brain were used; structural changes induced by Vc5 cytotoxin (CT) from Central Asia cobra venom have been studied by the EPR method using spin probes (5-, 10-, or 12-doxylstearic acid). The addition of CT to liposome samples, containing spin probes resulted in the appear...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
