Abstract:
:The effect of K+ and Na+ on the properties of DNA aptamers that selective binds to fibrinogen (FIBRI) and heparin (HEPA) exosites of human alpha-thrombin was studied by circular dichroism (CD). The complexes of FIBRI-K+ were slightly more stable than HEPA-K+. However, lower stability was observed for HEPA-K+ at presence of Na+ in comparison with FIBRI-K+. The analysis of CD melting curves suggests differences in thermal stability of both aptamers at presence of K+. The melting temperatures (Tm) and changes in van't Hoff enthalpy for HEPA-K+ complexes were lower in comparison with those for FIBRI-K+. With increasing HEPA concentration the Tm value increased, but Tm did not change with increasing FIBRI concentration. This suggests formation of HEPA aggregates, while FIBRI aptamers are in monomeric form.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Poniková S,Antalík M,Hianik Tsubject
Has Abstractpub_date
2008-12-01 00:00:00pages
271-7issue
4eissn
0231-5882issn
1338-4325journal_volume
27pub_type
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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更新日期:2003-03-01 00:00:00
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