Abstract:
:The purpose of this study was to evaluate the effect of resveratrol on cytogenetic damages of iododeoxyuridine (IUdR) and x-ray megavoltage radiation (6 MV) in spheroid model of U87MG glioblastoma cancer cell line using clonogenic and alkaline comet assay. Cells were cultured as spheroids (350 µm) that were treated with 20 μM resveratrol, 1 μM IUdR and 2 Gy of 6 MV x-ray. After treatment, viability of the cells, colony forming ability and the induced DNA damages were examined using trypan blue dye exclusion, colonogenic and alkaline comet assay, respectively. Our results showed that resveratrol could significantly reduce the colony number and induce the DNA damages of the cells treated with IUdR in combination with 6 MV x-ray radiation. That results indicated that resveratrol as an inhibitor of hypoxia inducible factor 1 alpha (HIF-1α) protein in combination with IUdR as a radiosensitizer enhanced the radiosensitization of glioblastoma spheroid cells.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Firouzi F,Khoei S,Mirzaei HRdoi
10.4149/gpb_2014023subject
Has Abstractpub_date
2015-01-01 00:00:00pages
43-50issue
1eissn
0231-5882issn
1338-4325journal_volume
34pub_type
杂志文章abstract::The aim of the present work was to test the sensitivity of young male Wistar rats, Dobrá Voda (Dv:WI) to the diabetogenic effect of streptozotocin (STZ) with regard to their health condition and mortality rates. Eight-week-old rats, weighing from 200 to 230 g, were randomised into five groups of eight animals. Strepto...
journal_title:General physiology and biophysics
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doi:
更新日期:1999-10-01 00:00:00
abstract::The polyamines spermine and spermidine and the diamine putrescine inhibit lipid peroxidation in phospholipid liposome suspensions and rat liver homogenates. Using the chemiluminescence technique the antioxidant activity of polyamines was found to be due to reactions with the free radical intermediates of lipid peroxid...
journal_title:General physiology and biophysics
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abstract::Although numerous studies have shown the existence of various types of ion conductance in antral part of gastric fundus mucosa epithelia of amphibian, practically no data are available on ion conductance in higher animal species. Present experiments were undertaken to check the possible existence of ion conductance in...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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abstract::Beat-to-beat variability of action potential duration (short-term variability, SV) is an intrinsic property of mammalian myocardium. Since the majority of agents and interventions affecting SV may modify also action potential duration (APD), we propose here the concept of relative SV (RSV), where changes in SV are nor...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015019
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doi:10.4149/gpb_2016029
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2011_01_34
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
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doi:
更新日期:2001-09-01 00:00:00
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journal_title:General physiology and biophysics
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doi:10.4149/gpb_2019032
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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abstract::The hydrolytic activity and interaction of acidic and neutral phospholipase A2 (PLA2) with large unilamellar liposomes treated with cobra venom cytotoxin Vc5 (CT Vc5) were studied to more fully understand the modulating effects of cationic membrane-active peptides on PLA2. Studies were done by fluorescence displacemen...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) is the major antioxidant in mitochondria that protect brain from neuroendocrine stress. Although MnSOD is localized in the mitochondria, the immediate subcellular distribution of MnSOD protein level in the prefrontal cortex and hippocampus of Wistar male rats exposed to acute str...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The postnatal development of high-affinity 3H-muscimol binding to GABAA receptors was studied in the lateral geniculate nucleus, superior colliculus, frontal and visual cortex of the rat brain. In the lateral geniculate nucleus 3H-muscimol binding rises from day 10 through day 37 reaching the highest value during the ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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abstract::Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
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abstract::Fusion of neuraminidase or trypsin pretreated human erythrocytes and untreated cells was performed. The obtained doublets had a permanent dipole moment which disappeared as a function of time. Since the dipole moment disappearance is due to protein interdiffusion the lateral diffusion constant may be determined. This ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
abstract::The voltage dependent ionic currents in cultured embryonic skeletal myocytes at stages of development ranging from 1 to 6 day were studied using the whole-cell patch clamp technique. Sodium (INa) and calcium (ICa) inward and potassium (IK) outward currents were observed at all stages. INa did not differ from that desc...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1993-06-01 00:00:00
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journal_title:General physiology and biophysics
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doi:10.4149/gpb_2017021
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journal_title:General physiology and biophysics
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journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::The action of the antiviral drug rimantadine on the structure of bilayer lipid membranes (BLM) and RBC membranes was investigated. Structural changes in BLM were recorded by ionophore conductivity changes and by changes in the third harmonic of capacity current signal due to lateral compression of BLM in an electric f...
journal_title:General physiology and biophysics
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doi:
更新日期:1989-02-01 00:00:00
abstract::The effect of melatonin on reperfusion arrhythmias and postischemic contractile dysfunction was studied in the isolated rat heart. 25 min global ischemia was induced and followed by 30 min of reperfusion. Melatonin (10 micromol/l) was present in the perfusion solution during the whole experiment. Experiment revealed p...
journal_title:General physiology and biophysics
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doi:
更新日期:2005-09-01 00:00:00
abstract::Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title:General physiology and biophysics
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doi:
更新日期:1994-06-01 00:00:00
abstract::7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2020022
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abstract::The aim of the present study was to investigate the ability of the chloroacetanilide herbicide acetochlor to interact with the endocrine system. The modulation of the binding of [3H]estradiol-17beta to protamine sulphate-precipitated uterine nuclear and cytoplasmic estrogen receptors was analysed for this purpose. Two...
journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:2000-03-01 00:00:00
abstract::In the present work have been made some modifications of Münch's model, which concerns the explanation of biophysical bases of the problem of transport of water and assimilates. Then, by using the thermodynamic formalism of Kedem and Katchalsky (1958), we have made a mathematical description of the model. On this basi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
abstract::Pretreatment with diazoxide, mitochondrial K(ATP) channel opener, was found to protect the rat heart against ischemia/reperfusion injury. Our aim was also to characterize the effects of diazoxide on the alterations of regulatory myocardial proteins, on mitochondrial ultrastructure, integrity and induction of apoptotic...
journal_title:General physiology and biophysics
pub_type: 杂志文章
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更新日期:2007-06-01 00:00:00