Abstract：
:Analogs of carbonyl cyanide phenylhydrazone providing no reaction with nucleophilic groups and lacking acidobasic properties, respectively, were synthesized for study of mechanism of uncoupling effect on oxidative phosphorylation. Their retention, influence on proton transport, abilities to SH--groups modify and to stimulate respiration in rat liver mitochondria, together with their physico-chemical properties, namely lipophilicity, acidobasicity and reactivity were characterized. The substitution of acidic hydrogen of the imino group resulted in the loss of both acidobasicity and uncoupling effect on oxidative phosphorylation. A decreased reactivity resulted from the substitutions of nitrile groups with the uncoupling activity remaining preserved.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Antalík M,Sturdík E,Sulo P,Propperová A,Mihalovová E,Podhradský D,Dzurila Msubject
Has Abstractpub_date
1988-10-01 00:00:00pages
517-28issue
5eissn
0231-5882issn
1338-4325journal_volume
7pub_type
杂志文章abstract：:Participation of electrostatic and other noncovalent interactions in the binding of tricyclic antidepressants (TCAs) to the lipid bilayers was estimated from pH-dependencies of imipramine, desipramine, amitriptyline and nortriptyline binding to the lipid bilayers prepared from different phospholipids, both electroneut...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:Thermal inactivation of peroxidase (POD) in an extract of Picea omorika (Pancić) Purkyne needles initiated by heat treatment was studied. This is the first study of this kind on a conifer species. Non-linear regression analysis was applied on the inactivation rate data, combining Mitscherlich and Arrhenius equations, ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_01_78
更新日期：2009-03-01 00:00:00
abstract：:Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, gui...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013016
更新日期：2013-06-01 00:00:00
abstract：:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zo...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:Gastric cancer (GC) is a high mortality disease. We studied the function and mechanism of long non-coding RNA prostate cancer-associated transcript 6 (lncRNA PCAT6) on cell proliferation and epithelial-mesenchymal transition (EMT) in GC cells. CCK-8, flow cytometry and colony formation assay were respectively used to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019044
更新日期：2020-01-01 00:00:00
abstract：:Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:Aiming at making the Münch model more adequate to the biological reality we introduce certain modifications and complements. Considering the model within the framework of so-called integrated system of long-distance water transport in plants we present a quantitative analysis based on the Kedem-Katchalsky formalism. A...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2001-06-01 00:00:00
abstract：:Cytochrome P-450 and NADPH: cytochrome P-450 reductase were solubilized by detergents from microsomal fraction of tulip bulbs Tulipa fosteriana L. and purified to electrophoretic homogeneity. The purification was achieved by anion-exchange column chromatography, hydroxyapatite-column chromatography and affinity chroma...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:Kinase-targeted therapy is a new and promising approach to disease treatment. However, some kinase inhibitors have been observed to cause an off-target adverse risk for skeletal system by influencing the growth of osteoblasts. It is known that the proliferation and differentiation of osteoblasts are essentially regula...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018030
更新日期：2019-01-01 00:00:00
abstract：:The human pituitary tumor-transforming gene-1 (hPTTG1) has been found to be overexpressed in various cancers. Accumulated evidences implicate that some of protein kinases can specifically recognize two PXXP motifs at hPTTG1 C-terminus through their Src homology (SH3) domain and then phosphorylate the protein by their ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016035
更新日期：2017-01-01 00:00:00
abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:S100A1 is a Ca2+-binding protein expressed at high levels in the myocardium. It is thought to modulate the Ca2+ sensitivity of the sarcoplasmic reticulum (SR) Ca2+ release channels (ryanodine receptors or RyRs) and its expression has been shown to be down regulated in various heart diseases. In this study we used S100...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2009_04_371
更新日期：2009-12-01 00:00:00
abstract：:The purpose of this study was to assess the possible protective effects of Artemisia campestris against fenthion-induced nephrotoxicity in adult rats and their progeny. Fenthion was administered orally at a dose of 551 ppm, which represented ¼ of LD50, for 21 consecutive days to pregnant and lactating rats. Oxidative ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013047
更新日期：2013-12-01 00:00:00
abstract：:The role of viscosity in coupling between chemical reaction (complex formation) and diffusion in membranes has been investigated. The Fick law was replaced by the momentum balance equation with the viscous term. The irreversible thermodynamics admits coupling of the chemical reaction rate with the gradient of velocity...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:The paper deals with mathematical modelling of the transients obtained by fitting of delayed fluorescence (DF) induction trace. The transients are in certain, doubtless connection with electrochemical gradient (ECG) formed across thylakoid membranes upon illumination. The fitting of the C and D transients by using con...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:The polyamines spermine and spermidine and the diamine putrescine inhibit lipid peroxidation in phospholipid liposome suspensions and rat liver homogenates. Using the chemiluminescence technique the antioxidant activity of polyamines was found to be due to reactions with the free radical intermediates of lipid peroxid...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:This study investigated time-dependent variations in the activities of adenosine deaminase (ADA), an adenosine-metabolizing enzyme, and myeloperoxidase (MPO), an oxidation reaction-catalyzing enzyme, in control and streptozotocin (STZ)-induced diabetic rat liver. The animals were sacrificed at six different times of d...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:The aim of the present work was to test the sensitivity of young male Wistar rats, Dobrá Voda (Dv:WI) to the diabetogenic effect of streptozotocin (STZ) with regard to their health condition and mortality rates. Eight-week-old rats, weighing from 200 to 230 g, were randomised into five groups of eight animals. Strepto...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
abstract：:This study examines the effects of a 2.1-GHz WCDMA-modulated microwave (MW) radiation on apoptotic activity and mitochondrial membrane potential (ΔΨm) in MCF-7 cells. The cells were exposed to the MW at a specific absorption rate (SAR) of 0.528 W/kg for 4 or 24 h. The antiproliferative effect of MW exposure was determ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016021
更新日期：2017-04-01 00:00:00
abstract：:Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017020
更新日期：2018-03-01 00:00:00
abstract：:A possible mechanism is put forward to explain the sliding of thin filaments during muscle contraction. In our model, repulsion due to electrostatic forces is the mechanism which triggers crossbridges to cause the thin filaments to slide. The mechanism proposed could operate regardless of whether the myosin heads rota...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Although the A1 adenosine receptor (A1 receptor), the main adenosine receptor type in cardiac muscle, is involved in powerful cardioprotective processes such as ischemic preconditioning, the atrial A1 receptor reserve has not yet been quantified for the direct negative inotropic effect of adenosine. In the present stu...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013041
更新日期：2013-09-01 00:00:00
abstract：:Diabetic cardiomyopathy is a severe complication of diabetes mellitus (DM). The goal of current work was to study the effects of sinomenine on streptozotocin-induced cardiomyopathy in rats. DM in rats was induced by intraperitoneal injection of streptozotocin. Cardiac function was evaluated by measuring left ventricle...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016033
更新日期：2017-01-01 00:00:00
abstract：:Aquaporin attracted attention not only of physiologists and biophysicists, but also of chemical engineers. Here we critically analyze a paper describing aquaporin-based artificial membranes, suggested for forward osmosis-based water purification (Wang et al. 2012, Small 8, pp. 1185-1190). Related papers published late...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013058
更新日期：2013-12-01 00:00:00
abstract：:The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:A number of computational methods for the description of the ligand-receptor interaction that were developed in the past decade are reviewed in this paper. The central two sections introduce the methods that are already established as useful tools for the qualitative and quantitative description of ligand-receptor com...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：1999-09-01 00:00:00
abstract：:Elevated serum resistin is implicated in insulin resistance associated with obesity and type 2 diabetes mellitus. Alcohol consumption interferes with the nutritional status, metabolic and hormonal activity of the drinker. Impact of ethanol intake on resistin level and resistin metabolic effects is unknown. Effect of l...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:This study investigated the noradrenergic contribution during the cutaneous vasoconstrictor response to local cooling in the leg and forearm. On each limb, one site was perfused with Yoh/Prop to block the postsynaptic adrenoceptors and another with Lactated Ringer's (control) using microdialysis. Blood flow was measur...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017021
更新日期：2018-01-01 00:00:00
abstract：:The effect of melatonin on reperfusion arrhythmias and postischemic contractile dysfunction was studied in the isolated rat heart. 25 min global ischemia was induced and followed by 30 min of reperfusion. Melatonin (10 micromol/l) was present in the perfusion solution during the whole experiment. Experiment revealed p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Lidocaine, a use-dependent Na(+) channel blocker, paradoxically evokes neural activation in the slowly adapting stretch receptor organ of crayfish at 5-10 mmol/l concentration. For elucidating the underlying mechanisms of this paradoxical effect, a series of conventional electrophysiological experiments were performed...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
