Abstract：
:In a previous paper we have described a 23 kD nuclear endonuclease (p23) that was mostly found to exist in a state of association with the isolated rat hepatocyte nuclear matrix. To investigate the nature of this interaction, the nuclear matrix was prepared using different procedures and examined for the presence/absence of the enzyme by activity gel analysis. Treatment of isolated nuclei with sodium tetrathionate (NaTT), a sulfhydryl-cross-linking agent, led to the complete recovery of p23 in the nuclear matrix, whereas incubation of nuclei with dithiothreitol (DTT), a sulfhydryl-reducing agent, led to its complete solubilization and resulting absence from the nuclear matrix. Exposure of the isolated nuclear matrix to DTT in high-ionic strength buffer, a procedure that promotes the solubilization of the internal nuclear matrix, caused the nearly complete solubilization of p23. It was concluded that disulfide bonds play an essential role in the association of p23 with the nuclear matrix and that p23 is mostly localized in the nuclear matrix interior.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Grdović N,Vidaković M,Poznanović Gsubject
Has Abstractpub_date
2005-03-01 00:00:00pages
99-111issue
1eissn
0231-5882issn
1338-4325journal_volume
24pub_type
杂志文章abstract：:Ischemic and reperfusion injury is a serious condition related to numerous biochemical and electrical abnormalities of the myocardium. It has been repeatedly studied in various animal models. In this study, the production of hydroxyl radicals and electrophysiological parameters were compared in three species. Rat, gui...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013016
更新日期：2013-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020002
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013024
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015018
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018047
更新日期：2019-03-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
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更新日期：2015-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019027
更新日期：2019-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
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doi：10.4149/gpb_2010_04_373
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013077
更新日期：2014-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013070
更新日期：2014-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016008
更新日期：2016-07-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015003
更新日期：2015-04-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016057
更新日期：2017-07-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
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更新日期：2015-10-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015019
更新日期：2016-01-01 00:00:00
