Abstract:
:In the present study, we evaluated the antioxidant potential of Artemisia campestris essential oil (ACEO) and the possible protective effects against deltamethrin induced hepatic toxic effects. The ACEO showed radical scavenging activity with IC50 = 47.66 ± 2.51 µg/ml, ferric reducing antioxidant power (FRAP) potential (EC50 = 5.36 ± 0.77 µg/ml), superoxide scavenging activity (IC50 = 0.175 ± 0.007 µg/ml) and ˙OH scavenging activity (IC50 = 0.034 ± 0.007 µg/ml). The obtained results of phenolic profile demonstrated that phenolic compounds are the major contributor to the antioxidant activity of ACEO. GC-MS analysis revealed the presence of 61 components in which monoterpene hydrocarbons constitute the major fraction (38.85%). In in vivo study, deltamethrin exposure caused an increase of serum AST, ALT and ALP activities, hepatic malondialdehyde (MDA) (measured as TBARS) and conjugated dienes markers of lipid peroxidation (LPO), while antioxidant enzyme activities (SOD, CAT and GPx) decreased significantly. Furthermore, it induces DNA damage as indicated by DNA fragmentation accompanied with severe histological changes in the liver tissues. The treatment with vitamin E or ACEO significantly improved the hepatic toxicity induced by deltamethrin. It can be concluded that vitamin E and ACEO are able to improve the hepatic oxidative damage induced by deltamethrin. Therefore, ACEO is an important product in reducing the toxic effects of deltamethrin.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Saoudi M,Ncir M,Ben Ali M,Grati M,Jamoussi K,Allouche N,El Feki Adoi
10.4149/gpb_2016057subject
Has Abstractpub_date
2017-07-01 00:00:00pages
331-342issue
3eissn
0231-5882issn
1338-4325journal_volume
36pub_type
杂志文章abstract::Currents through DPI 201-106 modified single cardiac sodium channels in guinea pig ventricular cells were measured using the patch clamp technique in the cell-free configuration to control the sodium concentrations on both sides of the patch membrane. Current-voltage relationships of the single channels were obtained ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1991-02-01 00:00:00
abstract::Lidocaine, a use-dependent Na(+) channel blocker, paradoxically evokes neural activation in the slowly adapting stretch receptor organ of crayfish at 5-10 mmol/l concentration. For elucidating the underlying mechanisms of this paradoxical effect, a series of conventional electrophysiological experiments were performed...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013077
更新日期:2014-01-01 00:00:00
abstract::Environmental exposure to modern microwave telecommunication electromagnetic fields (EMFs) has increased to unprecedented levels with consequent health complaints and concerns. Many studies have already reported genotoxic effects on a variety of organisms and cell/tissue types. Human peripheral blood lymphocytes from ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2019032
更新日期:2019-09-01 00:00:00
abstract::The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Inhibition of angiogenesis involves blocking of tyrosine kinases (TK) implicated in signalling of vascular endothelial growth factor receptors (VEFGR). The inhibition of TK results in a disruption of Ras/Raf/MEK/ERK1/2 and PI3K/Akt signalling pathways. We evaluated recently developed TK inhibitor 22SYM and compared it...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015055
更新日期:2016-10-01 00:00:00
abstract::Limited numbers of studies include research of microvibration therapy in experimental models. We examined effects of chronic vibroacustic-microvibration treatment on haemodynamics and anti-oxidative defense in experimental hypertension. Study was performed on chronically treated hypertensive and normotensive Wistar ra...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2014033
更新日期:2015-01-01 00:00:00
abstract::Tissue intermittent hypoxia (IH) occurs in obstructive sleep apnea, sickle cell anemia, physical exercise and other conditions. Poor gas solubility and slow diffusion through culture media hampers mimicking IH-induced transitions of O(2) in vitro. We aimed to develop a system enabling exposure of cultured cells to IH ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2014043
更新日期:2015-07-01 00:00:00
abstract::Arterial base deficit/excess (BD/E) is commonly used marker of metabolic acidosis in critically ill patients, but requires an arterial puncture and blood gas analysis. We hypothesized that serum bicarbonate (HCO3), which can be routinely obtained, strongly correlates with arterial BD/E and provides equivalent predicti...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Manganese superoxide dismutase (MnSOD) is the major antioxidant in mitochondria that protect brain from neuroendocrine stress. Although MnSOD is localized in the mitochondria, the immediate subcellular distribution of MnSOD protein level in the prefrontal cortex and hippocampus of Wistar male rats exposed to acute str...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The paper deals with mathematical modelling of the transients obtained by fitting of delayed fluorescence (DF) induction trace. The transients are in certain, doubtless connection with electrochemical gradient (ECG) formed across thylakoid membranes upon illumination. The fitting of the C and D transients by using con...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::The ionic composition of intracellular space is rigorously maintained in the expense of high-energy expenditure. It has been recently postulated that the cytoplasmic ionic composition is optimized so the energy cost of the fluctuations of calcium ion concentration is minimized. Specifically, thermodynamic arguments ha...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016029
更新日期:2017-04-01 00:00:00
abstract::The effects of thrombin stimulation on megakaryocytopoiesis and pulmonary-platelet interactions were investigated before and after administration of the compound to 15 mongrel dogs. Each dog served as its own control. Thrombin was given to encourage the traffic of megakaryocytes into the lung and to study the thrombin...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1984-08-01 00:00:00
abstract::In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2016039
更新日期:2016-10-01 00:00:00
abstract::Many herbicides employed in agriculture and also some antibiotics bind to a specific site of the reaction centre protein (RC) blocking the photosynthetic electron transport. Crystal structures showed that all these compounds bind at the secondary ubiquinone (QB) site albeit to slightly different places. Different herb...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015003
更新日期:2015-04-01 00:00:00
abstract::Homeostasis and development in vertebrates are regulated by cell proliferation, differentiation and death. Permeability of mitochondrial membranes, a decisive feature of apoptosis, is regulated by Bcl-2 family regulators. Protein p53 is able to reduce bcl-2 and promote bax expression. This study focused on the immunoh...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
abstract::We studied the involvement of O2, pH and low molecular thiols in H2S-induced decomposition of S-nitrosoglutathione (GSNO). The GSNO decomposition - •NO release was evaluated by UV-VIS spectroscopy and Griess assay. The H2S donor Na2S was used. O2 slightly increased, but was not necessary for the H2S-induced GSNO decom...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2013026
更新日期:2013-09-01 00:00:00
abstract::The effects of ryanodine on negative force staircase and potentiated rested-state contraction (RC) in rat myocardium were compared to the action of Ca release stimulator (caffeine) and inhibitors (local anesthetics). Only low ryanodine concentrations (0.1-0.5 mumol/l) were found to reverse anomalous mechanical pattern...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1988-02-01 00:00:00
abstract::A patient with a strongly lesioned spinal cord, sub C5, relearned running, besides improving other movements, by an oscillator formation training (rhythmic, dynamic, stereotyped exercise). After 45 days of jumping on a springboard and other rhythm trainings, the patient was able to run 90 m in 41 s (7.9 km/h) (even 9....
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::In this study, we investigated the role of AMP-activated protein kinase (AMPK) and the importance of reactive oxygen species (ROS) in apoptosis induction associated with cell cycle arrest induced by sulforaphane in AGS human gastric cancer cells. Our results demonstrated that sulforaphane inhibited proliferation of AG...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017026
更新日期:2018-03-01 00:00:00
abstract::The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptotheci...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2018017
更新日期:2018-09-01 00:00:00
abstract::The aim of the present work was to test the sensitivity of young male Wistar rats, Dobrá Voda (Dv:WI) to the diabetogenic effect of streptozotocin (STZ) with regard to their health condition and mortality rates. Eight-week-old rats, weighing from 200 to 230 g, were randomised into five groups of eight animals. Strepto...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1999-10-01 00:00:00
abstract::Although high-intensity electric fields (HEF) application is currently the only effective therapy available to terminate ventricular fibrillation, it may cause injury to cardiac cells. In this study we determined the relation between HEF pulse length and cardiomyocyte lethal injury. We obtained lethality curves by sur...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2015047
更新日期:2016-04-01 00:00:00
abstract::Lignin, the structural polymer of the plant cell walls, is produced by free radical polymerization of phenolic alcohols, catalyzed by different peroxidases. The mechanism and the structural organization of lignin in the cell have not been completely understood. In this study we applied fractal analysis to images of li...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2000-06-01 00:00:00
abstract::The complex formation between metmyoglobin and heparin was investigated by absorbance and fluorescence spectroscopy as well as differential scanning microcalorimetry. In acidic pH region, three distinct complexes detected by absorbance measurements are formed depending on pH and time of equilibration. The kinetics of ...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::The fibroblast growth factor receptors (FGFRs) and Ras/mitogen activated protein (RAS/MAP) signalling cascades are the main molecular pathways involved in breast carcinogenesis. This study aims to determine the association between FGF10 (rs4415084 C>T), FGFR2 (rs2981582 C>T) and MAP3K1 (rs889312 A>C) gene polymorphism...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017033
更新日期:2017-12-01 00:00:00
abstract::This study specified the role of several key calcium-operating ion channels in contraction/relaxation of human detrusor muscle as possible target for overactive bladder (OAB) treatment. Detrusor samples, obtained from 18 males (average age 61.5 ± 5.9 years), were investigated by organ tissue bath method with following...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:10.4149/gpb_2017064
更新日期:2018-07-01 00:00:00
abstract::Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::The action of the antiviral drug rimantadine on the structure of bilayer lipid membranes (BLM) and RBC membranes was investigated. Structural changes in BLM were recorded by ionophore conductivity changes and by changes in the third harmonic of capacity current signal due to lateral compression of BLM in an electric f...
journal_title:General physiology and biophysics
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00