Abstract：
:We studied the involvement of O2, pH and low molecular thiols in H2S-induced decomposition of S-nitrosoglutathione (GSNO). The GSNO decomposition - •NO release was evaluated by UV-VIS spectroscopy and Griess assay. The H2S donor Na2S was used. O2 slightly increased, but was not necessary for the H2S-induced GSNO decomposition. The rate of GSNO decomposition depended on pH; the maximum rate was observed at pH 7.4-8.0, and this decreased with lowering pH (6.4-4.5) as well as with increasing pH at 9.0-12.0. H2S-induced GSNO decomposition was slowed by the presence of other thiols, such as L-cysteine (Cys), N-acetyl-L-cysteine (NAC) and L-glutathione (GSH), but not in the presence of L-methionine (Met) or oxidized glutathione (GSSG). In sharp contrast, at pH 6.0, H2S-induced GSNO decomposition was negligible, yet the presence of Cys, NAC and GSH induced the H2S-driven GSNO decomposition (whilst Met and GSSG were inactive). In conclusion we postulate an involvement of low molecular thiols and pH in •NO signaling, by modulating the interactions of H2S with nitroso compounds, and hence in part they also appear to control H2S-triggered •NO release. The interaction of H2S and/or its derivatives with the thiol group may be responsible for the observed effects.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Grman M,Misak A,Jacob C,Tomaskova Z,Bertova A,Burkholz T,Docolomansky P,Habala L,Ondrias Kdoi
10.4149/gpb_2013026subject
Has Abstractpub_date
2013-09-01 00:00:00pages
429-41issue
3eissn
0231-5882issn
1338-4325journal_volume
32pub_type
杂志文章abstract：:Environmental exposure to modern microwave telecommunication electromagnetic fields (EMFs) has increased to unprecedented levels with consequent health complaints and concerns. Many studies have already reported genotoxic effects on a variety of organisms and cell/tissue types. Human peripheral blood lymphocytes from ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019032
更新日期：2019-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:It is currently believed that the protein folding rate is related to the protein structures and its amino acid sequence. However, few studies have been done on the problem that whether the protein folding rate is influenced by its corresponding mRNA sequence. In this paper, we analyzed the possible relationship betwee...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_02_154
更新日期：2011-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:The dynamics of a bioreactor with a variable volume and an active solute flux based on the thermodynamics of irreversible processes and stability analysis was studied. The active solute flux may control both the bioreactor volume and the hydrostatic pressure as well as the concentration of the solute inside the cell i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
abstract：:In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017007
更新日期：2017-10-01 00:00:00
abstract：:The mechanisms underlying the renoprotective effect for remote limb ischemic preconditioning (rIPC) against renal ischemia/reperfusion injury need further elucidation. In our work, one hundred and twenty male Sprague Dawley rats were randomized into 3 groups; sham, I/R group (left renal 45 min ischemia) and rIPC (as I...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015035
更新日期：2016-01-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017020
更新日期：2018-03-01 00:00:00
abstract：:For characterization of Na(+),K(+)-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of alpha1 subunit and the ATP- and Na(+)-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alterat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_03_266
更新日期：2010-09-01 00:00:00
abstract：:Tissue intermittent hypoxia (IH) occurs in obstructive sleep apnea, sickle cell anemia, physical exercise and other conditions. Poor gas solubility and slow diffusion through culture media hampers mimicking IH-induced transitions of O(2) in vitro. We aimed to develop a system enabling exposure of cultured cells to IH ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014043
更新日期：2015-07-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017064
更新日期：2018-07-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013058
更新日期：2013-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018017
更新日期：2018-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016001
更新日期：2016-07-01 00:00:00
abstract：:Calcium ions mediate extracellular signals on intracellular processes. The signalling system based on transient rises or oscillations of the cytoplasmic calcium concentration has potential advantages. The relevant mechanisms of intracellular concentration changes include calcium-induced calcium release and calcium dep...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:125I-labelled o-iodobenzoate (OIB) was prepared by means of an isotopic exchange reaction; its distribution and excretion were determined and its pharmacokinetic parameters in rats were calculated. The calculated value of the half-life of OIB elimination was 38.7 +/- 0.7 min, the distribution volume was 278.2 +/- 53.5...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：UNLABELLED:The effect of melatonin (MLT) on the isolated rat heart was studied using the standard perfusion conditions (Langendorff preparation) and model of calcium paradox (Ca(2+)-paradox). Ca(2+)-paradox was induced by 1 minute perfusion with Ca(2+)-free Krebs-Henseleit (KH) solution and subsequent 20 minutes perfus...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:A number of studies have reported that male reproductive organs are susceptible to electromagnetic fields (EMFs). The aim of this study was to explore the effects of microwave radiation exposures on 6-8 weeks old male Swiss albino mice. Mice were divided into two groups: group I - sham-exposed (Control), and group II ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017059
更新日期：2018-07-01 00:00:00
abstract：:In the present work have been made some modifications of Münch's model, which concerns the explanation of biophysical bases of the problem of transport of water and assimilates. Then, by using the thermodynamic formalism of Kedem and Katchalsky (1958), we have made a mathematical description of the model. On this basi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Ischemic/reperfusion brain injury (IRI) is a very severe event with the multiple etiopathogenesis. Ischemic preconditioning (IPC) is an important phenomenon of adaptation of CNS to subsequent ischemia. An altered cross-talk between intracellular calcium stores is presumed in the mechanisms of ischemic damage/protectio...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:The aim of this paper was to study the protein remodelling of the left ventricle following repeated administration of either daunorubicin (DNR) or DNR in combination with the cardioprotective agent dexrazoxane (DXZ). The experiment was carried out on three groups of Chinchilla male rabbits: 1. DNR (3 mg/kg i.v.), 2. D...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:Hyperlipidemia is found to be associated with changes in fatty acid (FA) profiles. The aim of this study was to investigate the effects of AHA-Step-1 dietary treatment and combination of fibrates (gemfibrozil) with dietary intervention on serum and erythrocyte phospholipid FA composition in human hyperlipidemia. 78 st...
journal_title：General physiology and biophysics
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:The character of structural changes in thylakoid membranes caused by temperature variation was investigated. Experiments were performed on maize leaf segments in vivo and in a closed temperature cycle 24-50-24 degrees C. Two biophysical methods were used for detection: luminescence and EPR. Arrhenius plots of delayed ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-08-01 00:00:00
abstract：:Antioxidative effects of nordihydroguaiaretic acid (NDGA) on murine bone marrow phagocytes were studied using luminol- and lucigenin-amplified chemiluminescence (CL). NDGA applied in vitro strongly suppressed the opsonized zymosan particles stimulated CL response in a dose-dependent manner (in concentrations of 0.3-30...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The postnatal development of high-affinity 3H-muscimol binding to GABAA receptors was studied in the lateral geniculate nucleus, superior colliculus, frontal and visual cortex of the rat brain. In the lateral geniculate nucleus 3H-muscimol binding rises from day 10 through day 37 reaching the highest value during the ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Thiamine deficiency (TD) results in lactate acidosis, which is associated with neurodegeneration. The aim of this study was to investigate this alteration in primary rat brain endothelia. Spectrophotometric analysis of culture media revealed that only a higher concentration of pyrithiamine, which accelerates the intra...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
