Abstract：
:This study specified the role of several key calcium-operating ion channels in contraction/relaxation of human detrusor muscle as possible target for overactive bladder (OAB) treatment. Detrusor samples, obtained from 18 males (average age 61.5 ± 5.9 years), were investigated by organ tissue bath method with following agents: diltiazem for L-type voltage-gated calcium channels; 3-fluropyridine-4-carboxylic acid (FPCA) for Orai-STIM channels; SKF 96365-hydrochloride for transient receptor potential (TRP) channels, T-type channels and Orai-STIM channels; 2- aminoethoxydiphenyl borate (2-APB) for inositol-triphosphate receptors (IP3Rs) and Orai-STIM channels. Oxybutynin and mirabegron were tested under the same conditions as controls. Mirabegron, 2-APB and FPCA exhibited the best suppressive effect on carbachol-induced detrusor contractility. As expressed by area under the contractile curve (AUCC), 2-APB, FPCA and mirabegron have similar AUCC: 1.79, 1.73, 1.73. The highest AUCC was 3.64 for diltiazem+SKF, followed by 3.21 for diltiazem, 3.16 for SKF and 2.94 for oxybutynin. The lowest median amplitude and contraction variability is for 2-APB followed by mirabegron and FPCA. There were significant differences between: 2-APB/FPCA vs.: ditiazem, diltiazem+SKF and SKF. Summary of results suggested the principal role of IP3Rs, Orai-STIM coupling and large-conductance calcium-activated potassium channels in detrusor contraction and pointed on Orai-STIM channels as possible targets for OAB pharmacotherapy.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Luptak J,Kocmalova M,Franova S,Sutovsky J,Grendar M,Svihra J,Kliment J Sr,Dusenka R,Sutovska Mdoi
10.4149/gpb_2017064subject
Has Abstractpub_date
2018-07-01 00:00:00pages
391-398issue
4eissn
0231-5882issn
1338-4325journal_volume
37pub_type
杂志文章abstract：:The aim of present study was to examine the impact of prenatal ethanol exposure on seizure susceptibility of the offspring. Pregnant Wistar rats were compelled to drink either 10% or 20% ethanol solution, as the only drinking fluid since conception up to the weaning of their offspring at the age of 28 days. Pregnant a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2012_019
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journal_title：General physiology and biophysics
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doi：
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abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:A simple graphical method is proposed to distinguish between two-state and gradual alterations in DNA conformation from circular dichroic spectra. The method is advantageous particularly to identify two-state conformational isomerizations which exhibit low degree of cooperativity. The usefulness of the method is illus...
journal_title：General physiology and biophysics
pub_type： 杂志文章
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更新日期：1986-08-01 00:00:00
abstract：:Recent findings suggest that polymorphisms in vitamin D pathway genes are candidates for association with multiple sclerosis susceptibility. It has been now well demonstrated that vitamin D has immunomodulatory functions that may be favorable for reduction of multiple sclerosis risk. Current research has been focused ...
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journal_title：General physiology and biophysics
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doi：10.4149/gpb_2015047
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journal_title：General physiology and biophysics
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doi：
更新日期：1991-10-01 00:00:00
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更新日期：1999-12-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013026
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb-2019057
更新日期：2020-03-01 00:00:00
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pub_type： 杂志文章
doi：10.4149/gpb_2020002
更新日期：2020-05-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：
更新日期：2005-06-01 00:00:00
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pub_type： 杂志文章
doi：10.4149/gpb_2019032
更新日期：2019-09-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
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doi：10.4149/gpb_2013077
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journal_title：General physiology and biophysics
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doi：
更新日期：1990-10-01 00:00:00
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doi：
更新日期：2009-01-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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doi：
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doi：
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017006
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journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
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journal_title：General physiology and biophysics
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journal_title：General physiology and biophysics
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