Abstract：
:The membrane lipid and fatty acid compositions of red blood cells from a paramyotonia patient were investigated. Cholesterol and total phospholipid contents in paramyotonia were not different from control. Only the sphingomyelin content was lower, and thus the molar ratio of phosphatidylcholine/sphingomyelin was higher than normal. The major abnormality concerned the fatty acid pattern. In all the phospholipid classes saturated fatty acids were increased and unsaturated fatty acids were decreased. The overall ratio of saturated/unsaturated fatty acids was 2.1 vs 1.6 in controls. Similar findings have been reported for the sarcolemma from paramyotonia patients. Thus, the results indicate that the membrane defect in this disease may be generalized.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Szymanska G,Marx A,Melzner I,Sarzala MG,Rüdel Rsubject
Has Abstractpub_date
1986-04-01 00:00:00pages
179-85issue
2eissn
0231-5882issn
1338-4325journal_volume
5pub_type
杂志文章abstract：:Diabetic cardiomyopathy is a severe complication of diabetes mellitus (DM). The goal of current work was to study the effects of sinomenine on streptozotocin-induced cardiomyopathy in rats. DM in rats was induced by intraperitoneal injection of streptozotocin. Cardiac function was evaluated by measuring left ventricle...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016033
更新日期：2017-01-01 00:00:00
abstract：:Recent studies have revealed the dipeptidyl peptidase IV (DPP IV) enzymatic activity of CD26 antigen. In this paper, the possible identity of DPP IV and CD26 expression in phenotypically defined T-ALL has been examined. The combination of enzyme cytochemistry and immunocytochemistry was used. The correlation between t...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Gastric cancer (GC) is a high mortality disease. We studied the function and mechanism of long non-coding RNA prostate cancer-associated transcript 6 (lncRNA PCAT6) on cell proliferation and epithelial-mesenchymal transition (EMT) in GC cells. CCK-8, flow cytometry and colony formation assay were respectively used to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019044
更新日期：2020-01-01 00:00:00
abstract：:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100, 200 or 400 ng/ml). Following 72-96 h storage at 4ºC, the sperm...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2011_SI1_36
更新日期：2011-01-01 00:00:00
abstract：:PC12 cells differentiated under the influence of the neuronal growth factor (NGF) serve as a model of both sympathetic neurons and chromaffin cells. NGF-induced differentiation critically depends on elevated intracellular calcium concentration. Main pathway for Ca²⁺ entry in excitable cells is represented by voltage-d...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014045
更新日期：2015-04-01 00:00:00
abstract：:The activity of different cathepsins and neutral proteinases was measured in normal and vitamin E-deficient rabbit muscles using specific substrates. Among the changes of enzyme activities in dystrophy caused by vitamin E-deficiency the increase in the activity of cathepsin B is the most striking. The activity of cath...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
abstract：:The aim of this study was to investigate the effect of oxidative stress on drug binding affinity, free and bound fraction. The fluorescence anisotropy method was used to examine the interactions of commercial human serum albumin (HSA) and human plasma proteins with ochratoxin A (OTA) or carboxylate form of camptotheci...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2018017
更新日期：2018-09-01 00:00:00
abstract：:The fibroblast growth factor receptors (FGFRs) and Ras/mitogen activated protein (RAS/MAP) signalling cascades are the main molecular pathways involved in breast carcinogenesis. This study aims to determine the association between FGF10 (rs4415084 C>T), FGFR2 (rs2981582 C>T) and MAP3K1 (rs889312 A>C) gene polymorphism...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017033
更新日期：2017-12-01 00:00:00
abstract：:Homocysteine (Hcys) may be implicated in different diseases, especially in cardiovascular illnesses. The most reactive form of Hcys is its cyclic thioester-homocysteine thiolactone (HTL), which is formed in plasma and represents up to 0.29% of plasma total Hcys. Recently, it has been observed that Hcys and HTL may mod...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_02_186
更新日期：2010-06-01 00:00:00
abstract：:Mitochondrial nucleoids (hereafter nucleoids) contain genetic information, mitochondrial DNA, prerequisite for mitochondrial functioning, particularly information required for mitochondrial electron transport. To understand nucleoid functioning, it is imperative to know its ultrastructure and dynamics in the context o...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016008
更新日期：2016-07-01 00:00:00
abstract：:7,8-Dihydroxyflavone (7,8-DHF) is a natural flavonoid compound that act as Trk-B agonist. 7,8-DHF is also a potent antioxidant. When applied systematically, 7,8-DHF can pass through blood-brain barrier and exhibit potential therapeutic effects in several animal models of neurodegenerative disorders. This study investi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2020022
更新日期：2020-09-01 00:00:00
abstract：:The effect of binding of an antitumour drug cis-diamminedichloroplatinum(II) (cis-[Pt(NH3)2Cl2]) to DNA on cutting effectiveness of BamHI, EcoRI, and SalI restriction endonucleases was quantitatively determined. The platinum complex inhibits the cleavage of plasmid pHC624 DNA linearized by BglI restrictase. From the p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:The capacity of five synthetic analogs of [8-arginine] vasopressin (AVP) to stimulate frog skin sodium transport (natriferic activity) was characterized electrophysiologically using the method of short-circuit current, and compared to that of synthetic AVP. The analogs used were [8-arginine] vasopressins modified in p...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:In the present study, we evaluated the antioxidant potential of Artemisia campestris essential oil (ACEO) and the possible protective effects against deltamethrin induced hepatic toxic effects. The ACEO showed radical scavenging activity with IC50 = 47.66 ± 2.51 µg/ml, ferric reducing antioxidant power (FRAP) potentia...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016057
更新日期：2017-07-01 00:00:00
abstract：:High Na+ + Ca2+ exchange rates comparable with those reported for crayfish striated muscle, rat heart and rat brain, were observed in locust striated muscle homogenates and membrane preparations. The Na(+)-Ca2+ exchange followed the 1st order kinetics with a Km value of 18 mumol.l-1 for Ca, the pH optimum was at 8, th...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:The postnatal developmental changes in mitochondrial uncoupling protein 1 (UCP 1) and F1-ATP synthase (ATPase) subunit levels in the interscapular brown adipose tissue (BAT) were studied in golden Syrian hamsters (Mesocricetus auratus) using electron microscopy in situ immunocytochemistry. The relatively low initial d...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:Recent findings suggest that polymorphisms in vitamin D pathway genes are candidates for association with multiple sclerosis susceptibility. It has been now well demonstrated that vitamin D has immunomodulatory functions that may be favorable for reduction of multiple sclerosis risk. Current research has been focused ...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2013067
更新日期：2013-12-01 00:00:00
abstract：:A growing body of evidence supports the role of free radicals in triggering the functional and metabolic disturbances following transient cerebral ischemia. This study was designed to evaluate whether the extent of reperfusion-induced inhibition of protein synthesis initiation as well as tissue injury can be reduced b...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
abstract：:This study investigated time-dependent variations in the activities of adenosine deaminase (ADA), an adenosine-metabolizing enzyme, and myeloperoxidase (MPO), an oxidation reaction-catalyzing enzyme, in control and streptozotocin (STZ)-induced diabetic rat liver. The animals were sacrificed at six different times of d...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:Apoptosis (programmed cell death) is an important process participating in the formation of organs and tissues during embryogenesis. Our aim of the work is studying the role of the apoptosis during the human embryonic differentiation. We tend to give acquired findings into the correlation with expression of proteins B...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:The aim of the study was to compare the effect of hemophane and polysulfone membranes on the phagocyte-derived production of reactive oxygen species (ROS) as well as on neutrophil CD11b and CD62L expression in patients undergoing regular hemodialysis. The effects of hemodialysis membranes were also studied in in vitro...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:Large-conductance chloride channels (maxi-Cl channels) were studied in cultured myoblasts (L6 rat muscle cell line); in excised (inside-out) and in cell attached membrane patches using a conventional patch clamp method. The incidence of maxi-Cl channels was substantially higher in proliferating myoballs, then in quies...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1994-06-01 00:00:00
abstract：:In P-gp-positive cell variants obtained from L1210 cells either by selection with vincristine (L1210/R) or by transfection with the human gene encoding P-gp (L1210/T), we have previously described cross-resistance to tunicamycin (TNM), a protein N-glycosylation inhibitor. Here we studied whether this cross-resistance ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016039
更新日期：2016-10-01 00:00:00
abstract：:Sodium nitroprusside (SNP) is a nitric oxide (•NO) donor in vitro and in vivo. In this paper the time variation of the intracellular water proton nuclear magnetic resonance (NMR) effective relaxation time T'(2a) in SNP-treated human erythrocyte suspensions, containing 10 mM membrane impermeable paramagnetic MnCl2, has...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_04_373
更新日期：2010-12-01 00:00:00
abstract：:This study examines the effects of a 2.1-GHz WCDMA-modulated microwave (MW) radiation on apoptotic activity and mitochondrial membrane potential (ΔΨm) in MCF-7 cells. The cells were exposed to the MW at a specific absorption rate (SAR) of 0.528 W/kg for 4 or 24 h. The antiproliferative effect of MW exposure was determ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2016021
更新日期：2017-04-01 00:00:00
abstract：:Physical exercise could cause muscle and tissue damage due to increase in the formation of free oxygen radicals during exercise. The aim of the present study was to investigate the effect of crocin on parameters associated with oxidative stress in recovery from acute swimming exercise in rats. Rats were divided into e...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2015018
更新日期：2016-01-01 00:00:00
abstract：:A model of activation of muscle contraction has been proposed. It is based on calcium diffusion and binding to specific regulatory sites in a sarcomere. Calcium ions activate interactions of contractile proteins and thus the generation of force. The model quantifies the relation between calcium released from intracell...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：UNLABELLED:The effect of melatonin (MLT) on the isolated rat heart was studied using the standard perfusion conditions (Langendorff preparation) and model of calcium paradox (Ca(2+)-paradox). Ca(2+)-paradox was induced by 1 minute perfusion with Ca(2+)-free Krebs-Henseleit (KH) solution and subsequent 20 minutes perfus...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-03-01 00:00:00
abstract：:CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
