Malate dehydrogenases--structure and function.

Abstract:

:Malate dehydrogenases (MDH, L-malate:NAD oxidoreductase, EC 1.1.1.37), catalyze the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate/aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. They are homodimeric molecules in most organisms, including all eukaryots and the most bacterial species. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure.

journal_name

Gen Physiol Biophys

authors

Minárik P,Tomásková N,Kollárová M,Antalík M

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

257-65

issue

3

eissn

0231-5882

issn

1338-4325

journal_volume

21

pub_type

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