Abstract：
:A simple graphical method is proposed to distinguish between two-state and gradual alterations in DNA conformation from circular dichroic spectra. The method is advantageous particularly to identify two-state conformational isomerizations which exhibit low degree of cooperativity. The usefulness of the method is illustrated on the examples of two synthetic DNA molecules which isomerize due to temperature changes or changes in salt concentration. The method can be used to characterize any phenomenon accompanied by alterations in optical activity.
journal_name
Gen Physiol Biophysjournal_title
General physiology and biophysicsauthors
Kypr J,Vorlícková Msubject
Has Abstractpub_date
1986-08-01 00:00:00pages
415-21issue
4eissn
0231-5882issn
1338-4325journal_volume
5pub_type
杂志文章abstract：:Binding of the tricyclic antidepressant imipramine (IMI) to neutral and negatively charged lipid membranes was investigated using a radioligand binding assay combined with centrifugation or filtration. Lipid bilayers were composed of brain phosphatidylcholine (PC) and phosphatidylserine (PS). IMI binding isotherms wer...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2004-03-01 00:00:00
abstract：:Malfunction of skeletal muscles and dysregulated turnover of sphingolipids in the insulin responsive tissues have been determined at old age. Present article investigates the role of acid sphingomyelinase (SMase)-dependent ceramide accumulation in reduction of the skeletal muscle sensitivity to insulin action at old a...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2017020
更新日期：2018-03-01 00:00:00
abstract：:Arachidonic, linolenic and linoleic acids decreased the binding of the m-cholinergic antagonist [3H] QNB and did not affect the ratio of high to low affinity binding sites to the agonist carbamoylcholine in rat brain synaptic membranes. In the presence of arachidonic acid, SH-reagent N-ethylmaleimide acquired the abil...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:Liposomes and proteoliposomes obtained from rat brain were used; structural changes induced by Vc5 cytotoxin (CT) from Central Asia cobra venom have been studied by the EPR method using spin probes (5-, 10-, or 12-doxylstearic acid). The addition of CT to liposome samples, containing spin probes resulted in the appear...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:Somatostatin analogues are currently used to treat various disorders such as hypersecretion and different neuroendocrine tumors. In this study we examined the effects on the adrenal cortex of somatostatin (SRIH-14) and octreotide administered subcutaneously twice daily for 5 days to adult male rats. Control rats recei...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2008-09-01 00:00:00
abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
abstract：:Recent findings suggest that polymorphisms in vitamin D pathway genes are candidates for association with multiple sclerosis susceptibility. It has been now well demonstrated that vitamin D has immunomodulatory functions that may be favorable for reduction of multiple sclerosis risk. Current research has been focused ...
journal_title：General physiology and biophysics
pub_type： 杂志文章,评审
doi：10.4149/gpb_2013067
更新日期：2013-12-01 00:00:00
abstract：:For characterization of Na(+),K(+)-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of alpha1 subunit and the ATP- and Na(+)-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alterat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2010_03_266
更新日期：2010-09-01 00:00:00
abstract：:Fasting for 48 h and the same period of recovery induced by 48 h refeeding increased rat hypothalamic monoamine oxidase (MAO) activity. However, in the interscapular brown adipose tissue (IBAT), only refeeding induced a significant elevation of the enzyme activity. As far as hypothalamic antioxidative enzymes are conc...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:The aim of the present study was to investigate the ability of the chloroacetanilide herbicide acetochlor to interact with the endocrine system. The modulation of the binding of [3H]estradiol-17beta to protamine sulphate-precipitated uterine nuclear and cytoplasmic estrogen receptors was analysed for this purpose. Two...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
abstract：:Gastric cancer (GC) is a high mortality disease. We studied the function and mechanism of long non-coding RNA prostate cancer-associated transcript 6 (lncRNA PCAT6) on cell proliferation and epithelial-mesenchymal transition (EMT) in GC cells. CCK-8, flow cytometry and colony formation assay were respectively used to ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2019044
更新日期：2020-01-01 00:00:00
abstract：:The role of C-type natriuretic peptide (CNP) in the gastrointestinal tract is still unclear. This study was designed to investigate the effect of CNP on barium current (I(Ba)) through the L-type calcium channel in gastric antral myocytes of guinea pigs. The whole-cell patch clamp technique was performed in gastric ant...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
abstract：:The hormone leptin, by binding to hypothalamic receptors, suppresses food intake and decreases body adiposity. Leptin receptors are also widely expressed in extra-hypothalamic areas such as hippocampus, amygdala and cerebellum, where leptin modulates synaptic transmission. Here we show that a defective leptin receptor...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013056
更新日期：2014-01-01 00:00:00
abstract：:The effects of thrombin stimulation on megakaryocytopoiesis and pulmonary-platelet interactions were investigated before and after administration of the compound to 15 mongrel dogs. Each dog served as its own control. Thrombin was given to encourage the traffic of megakaryocytes into the lung and to study the thrombin...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013077
更新日期：2014-01-01 00:00:00
abstract：:Two types of electrocardiographic data series were investigated using appropriate tests based on a selection of semi-quantitative analysis algorithms. Distribution histograms, power spectra, auto-correlation functions, state-space portraits, Lyapunov exponents and wavelet transformations were applied to electrocardiog...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:We studied physical properties of the monolayers and bilayer lipid membranes (BLM) formed by calix[6]arene carboxylic acid derivative (CX) and its mixtures with diphytanoylphosphatidylcholine (DPhPC) by means of measurement surface pressure, surface dipole potential and electrostriction. CX forms stable monolayers at ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2013024
更新日期：2013-06-01 00:00:00
abstract：:The left and right ventricles fulfill different role in heart function. Here we compare chamber specific changes in local catecholamine concentrations; gene expression and the receptor protein amount of all three β-adrenoceptors (β-AR) in rat right heart ventricles exposed to acute (1 session) and repeated (7 sessions...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：10.4149/gpb_2014002
更新日期：2014-01-01 00:00:00
abstract：:Hyperglycaemia-induced oxidative stress makes an important contribution to the aetiology of diabetic neuropathy. The aim of our study was to evaluate the effect of the antioxidant stobadine (STB) in comparison with a treatment by a high-dose of alpha-lipoic acid (ALA), on the neurological consequences of chronic hyper...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：:CCAAT/enhancer binding proteins (C/EBP) alpha, -beta and -delta play an important role in mediating I interleukin-6 (IL-6) dependent expression of acute-phase protein (APP) genes in liver during acute-phase (AP) response. Based on the presence of type IL-6 responsive element (IL-6 RE) in the rat haptoglobin (Hp) gene ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：:The paper deals with mathematical modelling of the transients obtained by fitting of delayed fluorescence (DF) induction trace. The transients are in certain, doubtless connection with electrochemical gradient (ECG) formed across thylakoid membranes upon illumination. The fitting of the C and D transients by using con...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:A method has been proposed for the kinetic analysis of the relaxation phase of mechanical response of smooth muscle. The method implies linearization of the entire mechano-kinetic relaxation curve using the coordinates ln[(fm-f)/f]; ln t (where fm and f are the maximal and the actual values of force within the relaxat...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Lidocaine, a use-dependent Na(+) channel blocker, paradoxically evokes neural activation in the slowly adapting stretch receptor organ of crayfish at 5-10 mmol/l concentration. For elucidating the underlying mechanisms of this paradoxical effect, a series of conventional electrophysiological experiments were performed...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:The relationship between erythrocyte shape and the critical cell volume was investigated. Agents able to increase the critical cell volume induced three main stable shapes of erythrocytes: discocytic, stomatocytic, and echinocytic. The absence of correlation between shape and critical cell volume under isoosmotic cond...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:A patient with a strongly lesioned spinal cord, sub C5, relearned running, besides improving other movements, by an oscillator formation training (rhythmic, dynamic, stereotyped exercise). After 45 days of jumping on a springboard and other rhythm trainings, the patient was able to run 90 m in 41 s (7.9 km/h) (even 9....
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Currents through DPI 201-106 modified single cardiac sodium channels in guinea pig ventricular cells were measured using the patch clamp technique in the cell-free configuration to control the sodium concentrations on both sides of the patch membrane. Current-voltage relationships of the single channels were obtained ...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:Stationary and time-dependent fluorescence were measured for a donor/ acceptor (DA) pair bound to membrane proteins of bovine erythrocyte ghosts. The donor N-(p-(2-benzoxazolyl)phenyl)-maleimide (BMI) and the acceptor fluram bind to SH- and NH2-residues, respectively. The fluorescence spectra and the time-dependent em...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zo...
journal_title：General physiology and biophysics
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
