Application of programmable, autonomously controlled electrode (PACE) technology to the development of an improved pulsed field gel electrophoresis assay for DNA double-strand breaks in mammalian cells.

abstract：:This study was designed to compare radiosensitization by the oral platinum compound JM216 with cisplatin. RIF1 mouse tumour cells were treated at various doses and at various exposure times with JM216 and irradiated 15 min before the end of drug exposure. The fraction of cells surviving treatment was assessed by colon...

journal_title：International journal of radiation biology

authors： van de Vaart PJ,Klaren HM,Hofland I,Begg AC

更新日期：1997-12-01 00:00:00

abstract：:The radiation response of 15 mammalian cell lines comprising 11 human tumour, two human fibroblast and two murine lymphoma cell lines, has been analysed using the linear-quadratic equation. As well as using conventional analysis of acute dose-survival curves to derive values for alpha and beta (termed alpha ac and bet...

journal_title：International journal of radiation biology

authors： Peacock JH,Eady JJ,Edwards SM,McMillan TJ,Steel GG

更新日期：1992-04-01 00:00:00

abstract：PURPOSE:To calculate survival of human haemopoietic stem cells irradiated by alpha particles from 149Tb (initial energy 3.97 MeV) and 211At (average initial energy 6.87 MeV). METHODS:Following as closely as possible radiobiological data, Monte Carlo methods were used to calculate passages of alpha particles (originati...

journal_title：International journal of radiation biology

authors： Charlton DE,Utteridge TD,Allen BJ

更新日期：1998-07-01 00:00:00

abstract：:Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...

journal_title：International journal of radiation biology

authors： Denekamp J,Whitmore GF,Jeggo P

更新日期：1989-04-01 00:00:00

abstract：PURPOSE:Gamma-irradiation leads to activation of p53 tumour suppressor gene and to p53-dependant stimulation of a large panel of cellular genes including proapoptotic genes involved in intrinsic and extrinsic pathways. Most in vivo published data referred to high (lethal) irradiation doses. The present study was perfor...

journal_title：International journal of radiation biology

authors： Alvarez S,Drané P,Meiller A,Bras M,Deguin-Chambon V,Bouvard V,May E

更新日期：2006-11-01 00:00:00

abstract：:The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...

journal_title：International journal of radiation biology

authors： Schäppi-Büchi C

更新日期：1994-04-01 00:00:00

abstract：:Eelpout (Zoarces viviparus), a marine fish, were exposed to gamma-radiation at a dose-rate of 2.0 mGy h-1. They were injected with the antigen trinitrophenyl-keyhole limpet haemocyanin (TNP-KLH) and their specific humoral immune response to the TNP hapten measured. There were no differences in the primary antibody res...

journal_title：International journal of radiation biology

authors： Greenwood LN,Knowles JF

更新日期：1995-01-01 00:00:00

abstract：:Recently, it has been demonstrated that two different thermal resistant states found in Chinese hamster cells, one transient, associated with thermotolerance, and the other permanent, associated with the increased expression of the cognate member of the hsp 70 family, are characterized by faster recovery from heat-ind...

journal_title：International journal of radiation biology

authors： Laszlo A,Davidson T,Hu A,Landry J,Bedford J

更新日期：1993-05-01 00:00:00

abstract：:Reasonable expectations, based on the normal physiology of the human fetus, cast new light on the possibilities of extrapolation from the finding of severe mental retardation (SMR) in Japanese bomb survivors after maternal exposure to 1.8-5.5 Gy T65DR Gy. After such large exposures the fetal haematopoietic tissues (DS...

journal_title：International journal of radiation biology

authors： Mole RH

更新日期：1990-10-01 00:00:00

abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...

journal_title：International journal of radiation biology

authors： Wallace SS

更新日期：1994-11-01 00:00:00

abstract：PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...

journal_title：International journal of radiation biology

authors： Liamsuwan T,Uehara S,Emfietzoglou D,Nikjoo H

更新日期：2011-02-01 00:00:00

abstract：PURPOSE:To determine whether murine lymphoid cell lines can discriminate between high- and low-LET (linear energy transfer) radiation-induced DNA lesions. MATERIALS AND METHODS:Sensitivity to killing by DNA-incorporated 3H and 125I decays, accumulated during storage in the gas phase of a liquid nitrogen tank, was dete...

journal_title：International journal of radiation biology

authors： Radford IR

更新日期：2002-06-01 00:00:00

abstract：PURPOSE:A meta-analysis was conducted to obtain a 'quantitative' estimate of the extent of genetic damage in mammalian somatic cells exposed to non-ionizing radiation emitted from extremely low frequency electro-magnetic fields (ELF-EMF) and to compare with that in unexposed control cells. METHODS:The methods used for...

journal_title：International journal of radiation biology

authors： Vijayalaxmi,Prihoda TJ

更新日期：2009-03-01 00:00:00

abstract：:A study has been made of the intrinsic radiosensitivity of peripheral blood lymphocytes from normal individuals. Cell survival following in vitro high (1.55 Gy min-1) and low (0.0098 Gy min-1) dose-rate irradiation was obtained for single lymphocyte samples from three individuals in six experiments. Despite wide inter...

journal_title：International journal of radiation biology

authors： Elyan SA,West CM,Roberts SA,Hunter RD

更新日期：1993-10-01 00:00:00

abstract：:Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...

journal_title：International journal of radiation biology

authors： Sunassee ED,Tan D,Ji N,Brady R,Moros EG,Caudell JJ,Yartsev S,Enderling H

更新日期：2019-10-01 00:00:00

abstract：PURPOSE:To investigate and compare the propensity of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL) obtained from unaffected healthy individuals and ataxia telangectasia (A-T) patients to undergo apoptosis after X-ray exposure. MATERIAL AND METHODS:The LCL were exposed to 1-4 Gy X-rays at a dose-...

journal_title：International journal of radiation biology

authors： Meijer AE,Zhivotovsky B,Lewensohn R

更新日期：1999-06-01 00:00:00

abstract：PURPOSE:Immune cells accumulate in and around cancers and cooperate with each other using specific cytokines to attack the cancer cells. The heavy-ion beams for cancer therapy may stimulate immune cells and affect on the immune system. However, it is still poorly understood how the immune cells are stimulated by ion-be...

journal_title：International journal of radiation biology

authors： Mutou-Yoshihara Y,Funayama T,Yokota Y,Kobayashi Y

更新日期：2012-03-01 00:00:00

abstract：:This study simulates the exposure of V79 Chinese hamster fibroblasts to low-energy protons, deuterons and alpha-particles in the LET range 10-200 keV/microm. The starting assumption is that the induction of clustered lesions in DNA is a fundamental step for cell inactivation. A non-homogeneous cell population was simu...

journal_title：International journal of radiation biology

authors： Ottolenghi A,Monforti F,Merzagora M

更新日期：1997-11-01 00:00:00

abstract：PURPOSE:Dose response and repair kinetics of phosphorylated histone H2A isoform X (gamma-H2AX) foci in T-lymphocytes were investigated in the low-dose range after in vitro irradiation of whole blood and T-lymphocytes with 100 kVp X-rays and (60)Co gamma-rays. MATERIALS AND METHODS:Whole blood or isolated T-lymphocytes...

journal_title：International journal of radiation biology

authors： Beels L,Werbrouck J,Thierens H

更新日期：2010-09-01 00:00:00

abstract：:The aim was to investigate further the relationship between radiation-induced mitotic delay and the expression of chromosome damage in V79 cells. Recently published data on the time-course of chromosome aberrations in V79 first-cycle metaphases after exposure to 10.4 MeV u(-1) Ar ions (LET = 1226 keV microm(-1)) were ...

journal_title：International journal of radiation biology

authors： Gudowska-Nowak E,Kleczkowski A,Nasonova E,Scholz M,Ritter S

更新日期：2005-01-01 00:00:00

abstract：:The accumulation and repair of radiation-induced DNA double-strand breaks (dsbs) were determined by neutral filter elution on 20 primary cultures obtained from ovarian cancer and malignant melanoma clinical specimens. The initial frequency of DNA dsbs after exposure to 50 Gy gamma-irradiation varied greatly for the in...

journal_title：International journal of radiation biology

authors： Zaffaroni N,Orlandi L,Villa R,Bearzatto A,Rofstad EK,Silvestrini R

更新日期：1994-09-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently dete...

journal_title：International journal of radiation biology

authors： Schmid E,Zitzelsberger H,Braselmann H,Gray JW,Bauchinger M

更新日期：1992-12-01 00:00:00

abstract：PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...

journal_title：International journal of radiation biology

authors： Boice JD Jr

更新日期：2017-10-01 00:00:00

abstract：:We characterized in vitro the effects of gamma-radiation (12.5-100 cGy) on pulmonary microvascular endothelial cell (PMEC) morphology and F-actin organization. Cellular retraction was documented by phase-contrast microscopy and the organization of actin microfilaments was determined by immunofluorescence. Characteriza...

journal_title：International journal of radiation biology

authors： Kantak SS,Diglio CA,Onoda JM

更新日期：1993-09-01 00:00:00

abstract：PURPOSE:To evaluate how irradiation affects thyroid autoimmunity in mouse models of Hashimoto's thyroiditis and Graves' hyperthyroidism. MATERIALS AND METHODS:Non-obese diabetic (NOD)-H2(h4) mice spontaneously develop anti-thyroglobulin (Tg) antibodies and thyroiditis when supplied with sodium iodine (NaI) in the drin...

journal_title：International journal of radiation biology

authors： Nagayama Y,Kaminoda K,Mizutori Y,Saitoh O,Abiru N

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:High dose ionizing radiation exposure is associated with myelo-depression leading to pancytopenia and the expected clinical manifestations of acute radiation syndrome (ARS). Herein, we evaluated the efficacy of sargramostim (Leukine®, yeast-derived rhu GM-CSF), with regimens delivered at 48, 72, 96, or 120 h...

journal_title：International journal of radiation biology

authors： Zhong Y,Pouliot M,Downey AM,Mockbee C,Roychowdhury D,Wierzbicki W,Authier S

更新日期：2020-09-22 00:00:00

abstract：PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...

journal_title：International journal of radiation biology

authors： Kim SR,Kim EH

更新日期：2017-05-01 00:00:00

abstract：:Purpose: Cancer is one of the most common causes of mortality and morbidity worldwide. Vaccines have been emerged as an attractive approach for their capacity of eliciting long-term immune response targeting cancer cells. Attenuated avirulent Toxoplasma gondii stimulate immunity and activate antitumor cells thereby el...

journal_title：International journal of radiation biology

authors： Hafez EN,Youssef HMG,El-Kabany HA

更新日期：2020-06-01 00:00:00

abstract：PURPOSE:To reduce retention and toxicity of the alpha particle emitter polonium-210 in rats by newly developed chelating agents. MATERIALS AND METHODS:Repeated subcutaneous chelation was conducted after intravenous injection of 210Po nitrate. For reduction of 210Po retention the treatment with vicinal dithiols meso-an...

journal_title：International journal of radiation biology

authors： Rencová J,Volf V,Jones MM,Singh PK

更新日期：2000-10-01 00:00:00

abstract：PURPOSE:The aim is to assess the change with age and disease of the oxygen concentration within the coronary artery walls. MATERIALS AND METHODS:In an accompanying paper, Part A, the age-dependent morphology and composition for the wall layers of normal and diseased coronary arteries were developed from published data...

journal_title：International journal of radiation biology

authors： Richardson RB

更新日期：2008-10-01 00:00:00