Abstract:
:This study simulates the exposure of V79 Chinese hamster fibroblasts to low-energy protons, deuterons and alpha-particles in the LET range 10-200 keV/microm. The starting assumption is that the induction of clustered lesions in DNA is a fundamental step for cell inactivation. A non-homogeneous cell population was simulated by a computer program, using as input measured morphological parameters reported in the literature. Variations in the number of traversals through each cell of the population and in the length of the traversal, depending on actual nuclear thickness and position of the traversal, the energy spread of the incident beam, and the change of LET along the tracks were included in the simulation. Microdosimetric spectra were computed and compared with spectra obtained neglecting particle slowing-down and stochastic aspects of cell morphology. Simulated cell survival was estimated under the assumption that surviving cells are those with no clustered DNA lesions or no passages. The main features of experimental RBE versus LET and particle type were reproduced by the simulations. The influence of stochastic aspects of target-cell morphology and of the energy of the incident particles on survival were investigated under different assumptions about the correlation between morphological parameters. Results support the hypothesis of a relevant role of clustered DNA damage in cell killing and point out the importance of target-cell morphology and its variability in beam dosimetry and computer simulations of low-energy particle radiation effects.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Ottolenghi A,Monforti F,Merzagora Mdoi
10.1080/095530097143004subject
Has Abstractpub_date
1997-11-01 00:00:00pages
505-13issue
5eissn
0955-3002issn
1362-3095journal_volume
72pub_type
杂志文章abstract::Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551051
更新日期:1995-08-01 00:00:00
abstract:PURPOSE:The hemorrhagic syndrome is a major cause of morbidity and mortality associated with the acute radiation syndrome (ARS). We previously characterized the dose-response relationship for total body irradiation (TBI)-induced ARS in the New Zealand White (NZW) rabbit. Thrombocytopenia, hemorrhage, and anemia were st...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1820601
更新日期:2020-10-16 00:00:00
abstract:PURPOSE:To evaluate and compare cytotoxic and mutational effects of graded doses of gamma-rays and 4He++ ions at different LET values (nominally 80 and 123 keV/microm) in V79 cells. MATERIALS AND METHODS:4He++ ion beams at 80 and 123 keV/microm were supplied by the 7 MV Van de Graaff CN accelerator of the INFN-LNL in ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000210146572
更新日期:2002-09-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently dete...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552621
更新日期:1992-12-01 00:00:00
abstract:PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701570196
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:Multicellular tumour spheroids are being used with increasing frequency in various aspects of tumour biology, including studies dealing with radiation biology. This review attempts to outline recent studies using these three-dimensional systems in radiation biology with particular reference made to papers testi...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530099139845
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:Recently, it was found that MK615 possessed an anti-proliferative ability on treated cancer cells as a consequence of triterpenoid compounds. It is well known that radiation affects cellular-mediated immunity in cancer patients who are treated with radiotherapy. Similarly, the ability of triterpenoid compounds ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518202
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110075220
更新日期:2001-12-01 00:00:00
abstract:PURPOSE:Adverse effects on human health caused by electromagnetic fields (EMF) associated with the use of mobile phones, particularly among young people, are increasing all the time. The potential deleterious effects of EMF exposure resulting from mobile phones being used in close proximity to the brain require particu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206223
更新日期:2016-10-01 00:00:00
abstract::Experimental data for acute radiation-induced skin reactions are reviewed. These show that for dose fractionation schedules with gaps, repopulation is initiated after a lag period. After this lag period, the isoeffective dose for a given level of skin reaction first increases rapidly, but then slows. The timing of the...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000310001600907
更新日期:2003-07-01 00:00:00
abstract:PURPOSE:To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. MATERIALS AND METHOD:Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050201073
更新日期:2000-12-01 00:00:00
abstract:PURPOSE:Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. MATERIALS AND METHODS:Macrocolony formation wa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.894650
更新日期:2014-05-01 00:00:00
abstract:PURPOSE:To evaluate the characteristic features of mechanical responses and the membrane potential changes induced by repetitive pulsed electromagnetic field (PEMF, 50 Hz, 5 mT) in thoracic aorta rings obtained from streptozotocin-induced diabetic and healthy control rats to determine if PEMF could ameliorate problems ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000903009522
更新日期:2009-08-01 00:00:00
abstract:PURPOSE:Following ionizing radiation exposure, intestinal crypt regeneration is possible but it is still not known if regenerating crypts give rise to differentiated functional epithelial cells on villi. The aim of this study was to demonstrate that irradiated progeny of enterocytic precursor cells are capable of proli...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500091923
更新日期:2005-03-01 00:00:00
abstract::Sample preparation procedures for the pulsed-field gel electrophoresis (PFGE) assay usually involve a lysis step at temperatures as high as 50 degrees C. During this warm-lysis procedure, multiply damaged sites containing heat-labile sites (HLS) can be converted into double-strand breaks (DSB). Once formed, these DSB ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500156577
更新日期:2005-06-01 00:00:00
abstract:PURPOSE:To determine the efficacy of a caffeine derivative 1-methylxanthine (1-MTX) in increasing radiosensitivity of cancer cells and elucidate the underlying mechanisms in vitro. MATERIALS AND METHODS:RKO human colorectal cancer cells carrying wild type protein 53 kDa (p53) were incubated with 3 mM 1-MTX for 30 min,...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000902741190
更新日期:2009-02-01 00:00:00
abstract::A radionuclide release assay for measuring the in vitro kinetics of cell death has been developed. CHO cells were labelled for 24 h with 3.0 hBq/ml of [125I] iododeoxyuridine (125IUdR) and the fate of the labelled cells and their progeny was monitored at daily intervals by measuring the rate of 125I release. Prelabell...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550361
更新日期:1991-02-01 00:00:00
abstract::In mice, external X- or gamma-irradiation may induce thymic lymphomas or myeloid leukaemias, while bone-seeking alpha-emitters may induce osteosarcomas and, to a lesser extent, acute myeloid leukaemia. The present paper aims to review briefly some of the experimental data with respect to the molecular mechanisms under...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014550851
更新日期:1990-04-01 00:00:00
abstract:PURPOSE:The space environment contains two major biologically significant influences; space radiations and microgravity. The 53 kDa tumour suppressor protein (p53) plays a role as a guardian of the genome through the activity of p53-centered signal transduction pathways. The aim of this study was to clarify the biologi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003789596
更新日期:2010-08-01 00:00:00
abstract::The radiation survival response of asynchronously-dividing populations of the cell line V79-WNRE indicates substructure in the low dose region that can be better characterized by separately fitting the data within the low and high dose regions to the linear-quadratic (LQ) equation. Flow cytometry and cell sorting tech...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551821
更新日期:1993-11-01 00:00:00
abstract::A comparison has been made of the in vitro light-activated drug cytotoxicities of two different porphyrin compounds, Photofrin II and TPPS4. An early passage human colon adenocarcinoma cell line, WiDr, has been exposed to either drug for 24 h, the excess drug washed from the cells and the cells irradiated with light u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552061
更新日期:1988-10-01 00:00:00
abstract:PURPOSE:To test the importance of serotonin as a signaling molecule involved in the production and response of radiation-induced bystander effects. MATERIALS AND METHODS:HPV-G human keratinocyte cultures were spiked with various concentrations of Granisetron or Ketanserin and subject to either 0 Gy or 0.5 Gy X-irradia...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.714519
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:To evaluate the radiomodulatory influence of a leaf extract of Mentha piperita (Linn) on hepatic antioxidant status and lipid peroxidation in Swiss albino mice. MATERIALS AND METHODS:Animals were given either double distilled water or leaf extract of M. piperita orally (1 g/kg bwt/day) once a day for three con...
journal_title:International journal of radiation biology
pub_type: 杂志文章,收录出版
doi:10.1080/09553000600771523
更新日期:2006-05-01 00:00:00
abstract::Solid human tumours contain areas with low oxygen tension (pO2). For bioreductive drugs it is important to define the cytotoxic effect according to drug concentration and to clinically relevant pO2. In this study, the pO2 dependence of the survival of three human cell lines (HRT 18, Na11 +, and MEWO), exposed to tirap...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550261
更新日期:1995-02-01 00:00:00
abstract::Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scorin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314552131
更新日期:1993-06-01 00:00:00
abstract::The repair of DNA double-strand breaks was measured by pH 7.2 filter elution in cells incubated at 25-45 degrees C either before or after X-irradiation. Exposure to 45 degrees C for 15 minutes immediately prior to X-irradiation significantly increased both the half-time for DNA double-strand break closure and the numb...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214550601
更新日期:1992-01-01 00:00:00
abstract:PURPOSE:The detection of long-term persistent chromosome aberrations in circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL AND METHODS:Peripheral blood samples from highly exposed persons were collected 7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and Chernobyl...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139395
更新日期:1999-10-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta 1) plays a central role in wound healing, so its perturbation by radiation may contribute to the acute and late effects seen in irradiated skin. TGF beta 1 mRNA expression was measured by PCR, in the skin of the CD1 and CBA mouse, exposed to Sr-90 beta from an 11-mm diameter s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551231
更新日期:1995-09-01 00:00:00
abstract:PURPOSE:Terrorist attacks, with their intent to maximize psychological and economic damage as well as inflicting sickness and death on given targeted populations, are an ever-growing worldwide concern in government and public sectors as they become more frequent, violent, and sensational. If given the chance, it is lik...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1332440
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:DNA damage is one of the main consequences of exposure to ionizing irradiation (IR). Recent studies indicate that IR can modulate the expression of immune system-related genes. However, the effects of IR on the expression of genes and pathways of the B7-CD28 superfamily remain poorly defined. The aim of this st...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1857454
更新日期:2020-12-17 00:00:00