Abstract:
PURPOSE:Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. MATERIALS AND METHODS:Macrocolony formation was assessed in primary human fibroblasts VH10 and HDFn with or without overlay using 0.5% agarose in growth medium at 24 h post-seeding. Malignant human cell lines (A549, U87) and transformed non-malignant fibroblasts (AA8 hamster, MRC5 human) were used for comparison. RESULTS:Agarose overlay caused significant improvement marked by early appearance (one week) of distinct colonies with high cell density and multifold higher plating efficiency than conventional macrocolony assay in VH10 and HDFn human fibroblasts. Compared to conventional assay or feeder cell supplementation, agarose overlay resulted in broader cell morphology due to improved adherence, and yielded more compact colonies. Gamma-radiation dose-response survival curves could be successfully generated for both fibroblast cell lines using this method, which yielded no such effects in the transformed/malignant cell lines tested. CONCLUSION:This easy and inexpensive 'agarose overlay technique' significantly and selectively improves the fibroblast plating efficiency, thus considerably reducing time and effort to greatly benefit the survival studies on primary fibroblasts.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Chandna S,Dagur RS,Mathur A,Natarajan AT,Harms-Ringdahl M,Haghdoost Sdoi
10.3109/09553002.2014.894650subject
Has Abstractpub_date
2014-05-01 00:00:00pages
401-6issue
5eissn
0955-3002issn
1362-3095journal_volume
90pub_type
杂志文章abstract::To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced c...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550661
更新日期:1995-05-01 00:00:00
abstract::The effect of a siderophore analogue 3,4,3-LIHOPO has been investigated in rat after intramuscular injection of 238Pu, 239Pu and 241Am simulating puncture wounds. Various treatment regimens were used to remove the radioactivity from its injection site and to reduce its retention in body tissues. The local deposits cou...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145382
更新日期:1996-07-01 00:00:00
abstract::In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In mos...
journal_title:International journal of radiation biology
pub_type:
doi:10.1080/095530097143220
更新日期:1997-10-01 00:00:00
abstract:PURPOSE:Adverse effects on human health caused by electromagnetic fields (EMF) associated with the use of mobile phones, particularly among young people, are increasing all the time. The potential deleterious effects of EMF exposure resulting from mobile phones being used in close proximity to the brain require particu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206223
更新日期:2016-10-01 00:00:00
abstract:PURPOSE:In case of a large-scale radiation accident when hundreds of people may be exposed, it is important to distinguish the severely exposed individuals (> or =1 gray), who require early medical treatment, from those less exposed. The aim of our study was to develop a quick population triage method based on automate...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553000903264481
更新日期:2010-01-01 00:00:00
abstract:PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110034649
更新日期:2001-05-01 00:00:00
abstract:PURPOSE:Since the number of cancer patients treated by proton irradiation has increased in the last few years, it seems appropriate to study dose-dependent effects of proton irradiation on mammalian tissues in more detail. MATERIALS AND METHODS:Tissue samples of normal skin of mouse and swine, of a human tumour model ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000412331283515
更新日期:2004-08-01 00:00:00
abstract::Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1571256
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:The relationship between cellular telephone use and excretion of the melatonin metabolite 6-hydroxymelatonin sulfate (6-OHMS) was evaluated in two populations of male electric utility workers (Study 1, n=149; Study 2, n=77). MATERIALS AND METHODS:Participants collected urine samples and recorded cellular telep...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000210166561
更新日期:2002-11-01 00:00:00
abstract::Cell cultures derived from skin biopsies of mouse fetuses X-irradiated at the zygote stage (2 Gy) were studied for the presence of chromosomal aberrations and micronuclei. In comparison with cells from control, unirradiated fetuses significantly higher spontaneous frequencies of aberrations per 100 metaphases and aber...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550091
更新日期:1989-01-01 00:00:00
abstract::Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1589013
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:Exposition of best practice in management and experimental use of human stem cell lines in radiobiological research. This paper outlines the key challenges to be addressed by radiobiologists wishing to use human pluripotent stem cell (hPSC) lines in their research including human embryonic stem cell (hESC) line...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.714518
更新日期:2012-10-01 00:00:00
abstract::A study has been made of the intrinsic radiosensitivity of peripheral blood lymphocytes from normal individuals. Cell survival following in vitro high (1.55 Gy min-1) and low (0.0098 Gy min-1) dose-rate irradiation was obtained for single lymphocyte samples from three individuals in six experiments. Despite wide inter...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551571
更新日期:1993-10-01 00:00:00
abstract::Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414551661
更新日期:1994-11-01 00:00:00
abstract:PURPOSE:To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data. MATERIALS AND METHODS:Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic exam...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141870
更新日期:1998-06-01 00:00:00
abstract:PURPOSE:5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530001453113
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000902836404
更新日期:2009-05-01 00:00:00
abstract:PURPOSE:Animal models that accurately reflect human responses to radiation injury are needed for advanced mechanistic investigation and development of effective therapeutics. The rabbit is an established animal model accepted by the FDA for studies of cardiovascular disease, lipid metabolism, the development of anticoa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1820606
更新日期:2020-10-26 00:00:00
abstract:PURPOSE:To test the importance of serotonin as a signaling molecule involved in the production and response of radiation-induced bystander effects. MATERIALS AND METHODS:HPV-G human keratinocyte cultures were spiked with various concentrations of Granisetron or Ketanserin and subject to either 0 Gy or 0.5 Gy X-irradia...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.714519
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:To illustrate the progress in normal tissue radiation biology over the last five decades and its impact on radiotherapy. MATERIALS AND METHODS:Major milestones over the last 50 years and their consequences for radiation oncology are described: The identification of clonogenic cell survival and the (target) ste...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000902985136
更新日期:2009-07-01 00:00:00
abstract::The behaviour of polyamines (putrescine, spermidine and spermine) in the small intestine was studied in rats exposed to a single whole-body dose of 3 Gy administered at four different times of the day. Polyamine content decreased a few hours after irradiation but quickly returned to normal values. Putrescine levels re...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551191
更新日期:1989-07-01 00:00:00
abstract::It is well established from clinical, epidemiological and laboratory studies that specific human germ line mutation can predispose to spontaneously arising cancer. Some of the responsible genes have been characterized at the molecular level and evidence is rapidly accumulating on mechanistic aspects of the problem. A ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414551761
更新日期:1994-11-01 00:00:00
abstract::The induction of chromosome aberrations by X-rays was investigated in synchronized quiescent and proliferating CHO-K1 cells. Kinetics of chromosome lesion repair was studied using the method of fractionated irradiation. In both cell types the time-course of repair during fractionation intervals followed first-order ki...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014552321
更新日期:1990-12-01 00:00:00
abstract::A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2018.1454616
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:To investigate the induction and persistence of chromosome aberrations in mouse bone marrow cells after X-ray exposure and to detect differential involvement of individual chromosomes in translocations. MATERIALS AND METHODS:Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome aberration...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139593
更新日期:1999-09-01 00:00:00
abstract:PURPOSE:Conditionally Replicative Adenovirus (CRAd) has been previously demonstrated to augment the activity of radiation, resulting in synergy of cell kill. However, previous models combining radiation with CRAd have not focused on the methods of radiation delivery. MATERIALS AND METHODS:We model the combination of a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553000903419338
更新日期:2010-03-01 00:00:00
abstract:PURPOSE:Estimating the absorbed dose to tumour relative to normal tissues has often been used in the assessment of the therapeutic efficacy of radiolabelled antibodies for radioimmunotherapy. Typically, the calculations assume a uniform dose deposition and response throughout the tumour. However, the heterogeneity of t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110117818
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. MATERIALS AND METHODS:Paddy field microcosm samples were continuously exposed at a dose rate o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.549534
更新日期:2011-07-01 00:00:00
abstract::This paper presents a summary of our understanding to date of the formation of DNA strand breaks induced by highly energetic particle beams (high-LET radiation). We have compared our own recent data on the formation of strand breaks induced in DNA in an aqueous solution with our previous data and those of others avail...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014551431
更新日期:1990-07-01 00:00:00
abstract::Genetic analysis was performed on 72 Italian A-T families ascertained through 91 probands. The frequency of the A-T gene was estimated through Dahlberg's formula, which uses the frequencies of first cousin marriages among the patients' parents and in the general population were obtained from the collection of Catholic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00