Abstract:
:The radiation survival response of asynchronously-dividing populations of the cell line V79-WNRE indicates substructure in the low dose region that can be better characterized by separately fitting the data within the low and high dose regions to the linear-quadratic (LQ) equation. Flow cytometry and cell sorting techniques have been used to determine the response of both asynchronous populations and synchronous populations obtained by mitotic selection with or without an additional drug block. The statistically significant substructure which is present in the radiation response of asynchronous cells is absent in G1/S cells synchronized by mitotic selection followed by hydroxyurea (or aphidicolin) accumulation at the G1/S boundary. Such G1/S populations have a radiation response that is well characterized by a single LQ expression over 3 logs of cell inactivation. In contrast, the radiation response of cells synchronized by mitotic selection alone and irradiated in early G1 shows substantial substructure when fitted to the LQ equation, reflecting some radioresistance at high dose. While this response is well described by a single hit plus multitarget equation, it is possible that the small proportion (< 5%) of interphase cells in such mitotically-selected populations may be responsible for the resistance observed at higher doses.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Skwarchuk MW,Wouters BG,Skarsgard LDdoi
10.1080/09553009314551821subject
Has Abstractpub_date
1993-11-01 00:00:00pages
601-12issue
5eissn
0955-3002issn
1362-3095pii
3DUJX4KGBBYHG66Rjournal_volume
64pub_type
杂志文章abstract:PURPOSE:To summarize current knowledge regarding mechanisms of radiation-induced skin injury and medical countermeasures available to reduce its severity. Advances in radiation delivery using megavoltage and intensity modulated radiation therapy have permitted delivery of higher doses of radiation to well-defined tumor...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2013.765055
更新日期:2013-05-01 00:00:00
abstract::Lethal mutations are induced in mammalian cells mainly, perhaps wholly, within the dose range encompassing the 'shoulder,' and may well be attributable to error-prone Q-repair. ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551961
更新日期:1988-10-01 00:00:00
abstract:PURPOSE:Adverse effects on human health caused by electromagnetic fields (EMF) associated with the use of mobile phones, particularly among young people, are increasing all the time. The potential deleterious effects of EMF exposure resulting from mobile phones being used in close proximity to the brain require particu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206223
更新日期:2016-10-01 00:00:00
abstract:PURPOSE:Hypoxic cells in tumours are resistant to 5-fluorouracil (5-FU). This in vivo study investigated the ability of hypoxia to regulate the gene expression of thymidylate synthase (TS), the target enzyme of 5-FU. MATERIALS AND METHODS:C3H mammary carcinomas, grown in the feet of female CDF1 mice, were used for all...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139944
更新日期:1999-07-01 00:00:00
abstract::Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0.1...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550791
更新日期:1995-06-01 00:00:00
abstract:PURPOSE:Multicellular tumour spheroids are being used with increasing frequency in various aspects of tumour biology, including studies dealing with radiation biology. This review attempts to outline recent studies using these three-dimensional systems in radiation biology with particular reference made to papers testi...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530099139845
更新日期:1999-07-01 00:00:00
abstract::The aim was to review and summarize the results of 40 years of study concerning the response to ionizing radiation of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in sensitivity to various DNA-damaging agents, among them X- and gamma-rays. The reviewed data indicate the key importance of DNA damage rep...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000500143534
更新日期:2005-05-01 00:00:00
abstract:PURPOSE:Exposition of best practice in management and experimental use of human stem cell lines in radiobiological research. This paper outlines the key challenges to be addressed by radiobiologists wishing to use human pluripotent stem cell (hPSC) lines in their research including human embryonic stem cell (hESC) line...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.714518
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:To investigate possible variations in topoisomerase (topo) I and II activities and levels after X-ray treatment in the radiation repair proficient AA8 Chinese hamster cell line for comparison with the radiation sensitive mutant EM9. MATERIALS AND METHODS:AA8 and EM9 cells were irradiated with 5 Gy of X-rays an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139809
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...
journal_title:International journal of radiation biology
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doi:10.3109/09553002.2014.926040
更新日期:2014-12-01 00:00:00
abstract::Only a small percentage of the DNA damage set by ionizing radiation in the living cell manifests itself as lethal. It is now increasingly accepted that clustered lesions may constitute the kind of damage that the repair enzymes cannot adequately deal with. The question is raised as to whether damage amplification reac...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414551501
更新日期:1994-11-01 00:00:00
abstract:PURPOSE:The LET position of the RBE maximum and its dependence on the cellular repair capacity was determined for carbon ions. Hamster cell lines of differing repair capacity were irradiated with monoenergetic carbon ions. RBE values for cell inactivation at different survival levels were determined and the differences...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139232
更新日期:1999-11-01 00:00:00
abstract::Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414551661
更新日期:1994-11-01 00:00:00
abstract::Sample preparation procedures for the pulsed-field gel electrophoresis (PFGE) assay usually involve a lysis step at temperatures as high as 50 degrees C. During this warm-lysis procedure, multiply damaged sites containing heat-labile sites (HLS) can be converted into double-strand breaks (DSB). Once formed, these DSB ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500156577
更新日期:2005-06-01 00:00:00
abstract::Dose responses for the effects of four calcium antagonists on SCCVII/St back and leg tumour sensitivity to 20 Gy X-rays, and on tumour perfusion indicated by relative uptake of 86rubidium, were determined. The effects of these agents on 86rubidium uptake in some normal tissues were also investigated. Flunarizine sensi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551511
更新日期:1989-09-01 00:00:00
abstract:PURPOSE:The aim of this work is to study the relative damaging effects of DNA-incorporated radionuclides by analyzing the behavior of the liquid-scintillation counting efficiency in volumes of nanometer size. METHODS:A liquid scintillation counter can detect changes in the micelle size when different percentages of an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802460156
更新日期:2008-12-01 00:00:00
abstract:PURPOSE:A minority of patients exhibits severe late normal tissue toxicity after radiotherapy (RT), possibly related to their inherent individual radiation sensitivity. This study aimed to evaluate four different candidate in vitro cellular radiosensitivity assays for prediction of late normal tissue reactions, in a re...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1230238
更新日期:2016-12-01 00:00:00
abstract:PURPOSE:To investigate whether pre- and post-drug magnetic field (MF) exposure of 50 Hz, 0.2 mT has any significant effect on pentylenetetrazol (PTZ)-induced seizures in mice. MATERIAL AND METHODS:MF was generated by a pair of Helmholtz coils. Seizures were induced by PTZ injection intraperitoneally (i.p.) at a dose o...
journal_title:International journal of radiation biology
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doi:10.1080/09553000701206676
更新日期:2007-04-01 00:00:00
abstract::Purpose: A study is presented of the irradiation of cancerous cervical cell line HeLa loaded with a platinum salt, betamethasone and deoxyglucose. The presence of the platinum increases the free-radical concentration and augments the cell death rate, whereas betamethasone or deoxyglucose induces radiosensitization by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1721594
更新日期:2020-05-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550731
更新日期:1991-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
abstract::Most estimates of the radiation dose lethal to 50 per cent of a human population are based on historical data taken from well-known experiences reflecting inadequately known physical and biological conditions, or from medical procedures where individual patients received advantages of modern clinical care. It has been...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550481
更新日期:1989-03-01 00:00:00
abstract::We used a cell sorter assay to evaluate the efficacy of the hypoxic cell sensitizer etanidazole over 3-4 logs of cell inactivation, with particular attention to the clinically relevant low-dose survival region. Analysis of the radiation responses in a panel of six human tumour cell lines under conditions of hypoxia an...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144617
更新日期:1996-12-01 00:00:00
abstract::Because of the role that alveolar macrophages (PAM) play in the pulmonary clearance of inhaled particles via mechanical transport and dissolution, understanding the uptake and dissolution of particles by these cells might provide insight into the mechanisms of particle dissolution in lungs of various species and hence...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551861
更新日期:1992-07-01 00:00:00
abstract::Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145634
更新日期:1996-05-01 00:00:00
abstract:PURPOSE:To investigate the radiosensitisation of gold nanoparticles (GNP) with an average diameter of 5 nm coated with the gadolinium chelating agent dithiolated diethylenetriaminepentaacetic gadolinium (Au@DTDTPA:Gd) in vitro and in mice bearing tumours (MC7-L1). MATERIALS AND METHODS:In vivo, the gadolinium chelate ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003746067
更新日期:2010-08-01 00:00:00
abstract::To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced c...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550661
更新日期:1995-05-01 00:00:00
abstract:PURPOSE:To investigate the consequence of continuous low dose-rate exposure to gamma-rays on ornithine decarboxylase (ODC EC 4.1.1.17) activity in organs of rat. MATERIALS AND METHODS:Young male Wistar rats were irradiated at 1.1, 2.1 and 12.9 cGy/day in the dose ranges of 9-165, 17-315 and 100-2000 cGy, respectively,...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140645
更新日期:1999-02-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.809170
更新日期:2013-11-01 00:00:00