Abstract:
PURPOSE:Exposition of best practice in management and experimental use of human stem cell lines in radiobiological research. This paper outlines the key challenges to be addressed by radiobiologists wishing to use human pluripotent stem cell (hPSC) lines in their research including human embryonic stem cell (hESC) lines and human induced pluirpotency stem (hiPSC) lines. It emphasises the importance of guidance already established for cell culture in general and outlines some further considerations specific to the culture of human pluripotent stem cell lines which may impact on the interpretation of data from radiobiological studies using these cells. Fundamental standards include obtaining cells from bona fide suppliers with suitable quality controls, screening cell lines to ensure absence of mycoplasma and authentication of cell lines by DNA profiling. For hESC and hiPSC lines, it is particularly important to recognise the significance of phenotypic and genetic stability and this paper will address approaches to reduce their impact. Quality assured banking of these two types of stem cell lines will facilitate reliable supply of quality controlled cells that can provide standardisation between laboratories and in the same laboratory over time. CONCLUSIONS:hPSC lines could play an important role in future radiobiological research providing certain fundamental principles of good stem cell culture practice are adopted at the outset of such work.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Stacey Gdoi
10.3109/09553002.2012.714518subject
Has Abstractpub_date
2012-10-01 00:00:00pages
703-8issue
10eissn
0955-3002issn
1362-3095journal_volume
88pub_type
杂志文章abstract:PURPOSE:To investigate the radiosensitisation of gold nanoparticles (GNP) with an average diameter of 5 nm coated with the gadolinium chelating agent dithiolated diethylenetriaminepentaacetic gadolinium (Au@DTDTPA:Gd) in vitro and in mice bearing tumours (MC7-L1). MATERIALS AND METHODS:In vivo, the gadolinium chelate ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003746067
更新日期:2010-08-01 00:00:00
abstract::Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551051
更新日期:1995-08-01 00:00:00
abstract:PURPOSE:The recent rapid increase of hadron therapy applications requires the development of high performance, reliable in vivo models for preclinical research on the biological effects of high linear energy transfer (LET) particle radiation. AIM:The aim of this paper was to test the relative biological effectiveness ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1511928
更新日期:2018-11-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:The detection of long-term persistent chromosome aberrations in circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL AND METHODS:Peripheral blood samples from highly exposed persons were collected 7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and Chernobyl...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139395
更新日期:1999-10-01 00:00:00
abstract:BACKGROUND:High dose ionizing radiation exposure is associated with myelo-depression leading to pancytopenia and the expected clinical manifestations of acute radiation syndrome (ARS). Herein, we evaluated the efficacy of sargramostim (Leukine®, yeast-derived rhu GM-CSF), with regimens delivered at 48, 72, 96, or 120 h...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1673499
更新日期:2020-09-22 00:00:00
abstract:PURPOSE:The deleterious processes triggered by heavy ions on DNA were studied through the determination of the yield of a series of oxidized bases. Emphasis was placed on the estimation of the respective contribution of direct ionization and indirect effects, mostly by comparison with low linear energy transfer (LET) g...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600573788
更新日期:2006-02-01 00:00:00
abstract:PURPOSE:To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. MATERIALS AND METHODS:Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20 h ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139700
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802499212
更新日期:2008-11-01 00:00:00
abstract:PURPOSE:The tandemly repeated hexamers (TTAGGG)n present at the telomeric ends protect the human genome from a variety of environmental exposures including ionizing radiation. In order to find out the effect of chronic low dose radiation exposure, we have determined telomere length among newborns from high level natura...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.699135
更新日期:2012-09-01 00:00:00
abstract:PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140474
更新日期:1999-03-01 00:00:00
abstract:PURPOSE:To determine the involvement of c-Jun NH(2)-terminal kinase-1 (JNK1) and possibly of HSP27 in heat-induced apoptosis of human monoblastic leukaemia U937 cells. MATERIALS AND METHODS:Dominant negative JNK1 (APF), in which the phosphorylation sites Thr-Pro-Tyr were changed to Ala-Pro-Phe, was overexpressed in U9...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110062512
更新日期:2001-08-01 00:00:00
abstract::The repair activity of verbascoside (VER), isolated from Pedicularis spicata, towards the oxidizing hydroxyl radical adduct of dGMP and its reaction mechanism were studied using pulse radiolysis. Upon pulse radiolysis of nitrous oxide saturated aqueous solution of 2'-deoxyguanosine-5'-monophosphate (dGMP) and VER, it ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145779
更新日期:1996-04-01 00:00:00
abstract:PURPOSE:The impact of the damage distribution to cellular survival and chromosomal aberrations following high Linear Energy Transfer (LET) radiation was investigated. MATERIALS AND METHODS:High LET iron-ions (500 MeV/n, LET 200 keV/μm) were delivered to G1-phase synchronized Chinese Hamster Ovary (CHO) cells located a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.767990
更新日期:2013-06-01 00:00:00
abstract::The form of the hazard function for radiation-induced pneumonitis death in mice was investigated. 'Hazard' refers to the instantaneous failure rate at a specified time, conditional upon non-failure to that time. Thus, the hazard function describes the time profile for the risk of pneumonitis death among still-survivin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552551
更新日期:1992-11-01 00:00:00
abstract:PURPOSE:The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1101500
更新日期:2016-01-01 00:00:00
abstract::The relationship between ionizing radiation-induced cell killing and DNA damage measured by the micronucleus and apoptosis assays was determined in three established cell lines (L929, HL-60, and Chang). Irradiation experiments revealed a dose-dependent increase of micronucleated cells until a certain dose was reached....
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550371
更新日期:1995-03-01 00:00:00
abstract:PURPOSE:To determine the relationships between the frequencies of radiation-induced chromosomal alterations and the extent of apoptosis in G0 human lymphocytes. MATERIAL AND METHODS:G0 human peripheral blood lymphocytes (HPBL) were X or gamma-irradiated, in the presence or absence of the repair inhibitor cytosine arab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500303690
更新日期:2005-08-01 00:00:00
abstract:PURPOSE:Cartilage degradation usually results as a consequence of inflammatory processes in the joints. To study this phenomenon experimentally, adjuvant-induced arthritis (AIA) was used as a model of chronic inflammation under the influence of irradiation. The potential chondroprotective effect of 13% aqueous extract ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.530337
更新日期:2011-03-01 00:00:00
abstract:PURPOSE:To describe the design and dosimetric characterization of a simple and economical small animal irradiator. MATERIALS AND METHODS:A high dose rate (HDR) (192)Ir brachytherapy source from a commercially available afterloader was used with a 1.3 cm thick tungsten collimator to provide sharp beam penumbra suitable...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.922719
更新日期:2014-10-01 00:00:00
abstract::Purpose: Projects evaluating the effects of radiation, within the National Institutes of Quantum and Radiological Science and Technology (QST), National Institute of Radiological Sciences (NIRS), have focused on risk analyses for life shortening and cancer prevalence using laboratory animals. Genetic and epigenetic al...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1625458
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:The present study was designed to reveal, whether the 30 min daily full body exposure of mice to an inhomogeneous static magnetic field (SMF) has a statistically significant effect on diabetic neuropathy. MATERIALS AND METHODS:Three different doses (100, 150, or 200 mg/kg) of streptozotocin (STZ) were administ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518200
更新日期:2011-01-01 00:00:00
abstract:PURPOSE:To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia. MATERIALS AND METHODS:Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110043711
更新日期:2001-06-01 00:00:00
abstract::Primary chick embryo myoblasts can be a useful tool for studying the developmental events which accompany myoblast differentiation, particularly myoblast membrane fusion. To determine whether the electrical properties and/or fusion in these systems are affected by 50 Hz magnetic fields, chick embryo myoblast cultures ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Skin fibroblasts from ataxia telangiectasia (AT) patients, obligate AT heterozygotes (ATH) and normal individuals were studied for colony-forming ability and repair of DNA double-strand breaks (dsb) after gamma-irradiation. AT cells were three to four times more radiosensitive than normal cells at high and low dose-ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552601
更新日期:1991-11-01 00:00:00
abstract:PURPOSE:To develop and validate non-fluorescent chromosome painting for bright-field microscopy using the peroxidase/diaminobenzidine (DAB) reaction. MATERIALS AND METHODS:Peripheral blood lymphocytes were taken from patients with uterine cancer who had received heavy-ion radiation therapy. Chromosome slides were trea...
journal_title:International journal of radiation biology
pub_type:
doi:10.1080/095530098142077
更新日期:1998-05-01 00:00:00
abstract:PURPOSE:To investigate within the framework of a multilaboratory study the suitability of FISH chromosome painting to measure so-called stable translocations in peripheral lymphocytes of Mayak nuclear-industrial workers (from the Southern Urals) and their use for retrospective biodosimetry. MATERIALS AND METHODS:Chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,多中心研究
doi:10.1080/09553000010018693
更新日期:2001-03-01 00:00:00
abstract:PURPOSE:To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. MATERIALS AND METHOD:Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050201073
更新日期:2000-12-01 00:00:00
abstract::Radiation-induced myocardial degeneration in the rat is preceded by changes in capillary structure and function, which may be a major factor in the pathogenesis of radiation-induced heart disease. In order to investigate the mechanism of capillary damage we studied endothelial cell proliferation in untreated control r...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551131
更新日期:1990-05-01 00:00:00
abstract:PURPOSE:To investigate the ability of electromagnetic (EM) field pre-exposures to induce protection in chick embryos against subsequent ultraviolet (UV) light exposure. MATERIALS AND METHOD:Chick embryos in the 4th day of gestation were exposed for 20 minutes (short term) or 96 hours (long term) to 60 Hz, 8 microT mag...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139142
更新日期:1999-12-01 00:00:00