Abstract:
:Purpose: Projects evaluating the effects of radiation, within the National Institutes of Quantum and Radiological Science and Technology (QST), National Institute of Radiological Sciences (NIRS), have focused on risk analyses for life shortening and cancer prevalence using laboratory animals. Genetic and epigenetic alterations in radiation-induced tumors have been also analyzed with the aim of better understanding mechanisms of radiation carcinogenesis. As well as the economic and practical limitations of repeating such large-scale experiments, ethical considerations make it vital that we store and share the pathological data and samples of the animal experiments for future use. We are now constructing such an archive called the Japan-Storehouse of Animal Radiobiology Experiments (J-SHARE). Methods: J-SHARE records include information such as detailed experimental protocols, necropsy records and photographs of organs at necropsy. For each animal organs and tumor tissues are dissected, and parts are stored as frozen samples at -80 °C. Samples fixed with formalin are also embedded in paraffin blocks for histopathological analyses. Digital copies of stained tissues are being systematically saved using a virtual slide system linked to original records by barcodes. Embedded and frozen tissues are available for molecular analysis. Conclusion: Similar archive systems for radiation biology have also been under construction in the USA and Europe, the Northwestern University Radiation Archive (NURA), and STORE at the BfS, respectively. The J-SHARE will be linked with the sister-archives and made available for collaborative research to institutions and universities all over the world.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Morioka T,Blyth BJ,Imaoka T,Nishimura M,Takeshita H,Shimomura T,Ohtake J,Ishida A,Schofield P,Grosche B,Kulka U,Shimada Y,Yamada Y,Kakinuma Sdoi
10.1080/09553002.2019.1625458subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1372-1377issue
10eissn
0955-3002issn
1362-3095journal_volume
95pub_type
杂志文章,评审abstract:PURPOSE:γ-rays (IR) cause an increase in intracellular calcium [Ca(2+)], alters contractility and triggers apoptosis via the activation of protein kinase C in intestinal guinea pig smooth muscle cells. The present study investigated the role of the mitochondria in these processes and characterized proteins involved in ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.911988
更新日期:2014-10-01 00:00:00
abstract::Despite the extensive utilization of radiotherapy in cancer treatments, clinicians often face challenges in achieving desired outcomes. This is because of the existence of secondary radiation effects like bystander phenomenon that influence radiation nontargeted cells and tissues. The concept of bystander effects of r...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2019.1547440
更新日期:2019-03-01 00:00:00
abstract:PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701317366
更新日期:2007-06-01 00:00:00
abstract:PURPOSE:Radiation-induced bystander effects have important implications in radiotherapy. Their persistence in normal cells may contribute to risk of health hazards, including cancer. This study investigates the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of harmful e...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.950715
更新日期:2015-01-01 00:00:00
abstract::Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently dete...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552621
更新日期:1992-12-01 00:00:00
abstract::The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550501
更新日期:1994-04-01 00:00:00
abstract:PURPOSE:In radiation biology, rats are often irradiated, but the precise dose distributions are often lacking, particularly in areas that receive scatter radiation. We used a non-dedicated set of resources to calculate detailed dose distributions, including doses to peripheral organs well outside of the primary field, ...
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doi:10.3109/09553002.2013.791407
更新日期:2013-09-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050029011
更新日期:2000-06-01 00:00:00
abstract:PURPOSE:To propose an improved version of RADACK, a stochastic simulation of radiolytic attack on DNA, that takes into account the reactivity of each amino acid of a specifically bound protein with hydroxyl radicals. To apply it to the natural lactose operator-repressor complex taking advantage of recently reported str...
journal_title:International journal of radiation biology
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doi:10.1080/0955300031000137788
更新日期:2003-06-01 00:00:00
abstract:PURPOSE:Human diseases associated with acute radiation responses are rare genetic disorders with common clinical and biological features including radiosensitivity, genomic instability, chromosomal aberrations, and frequently immunodeficiency. To determine what molecular assays are predictive of cellular radiosensitivi...
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doi:10.1080/09553000701797039
更新日期:2008-02-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001596959
更新日期:2003-08-01 00:00:00
abstract::Genetic analysis was performed on 72 Italian A-T families ascertained through 91 probands. The frequency of the A-T gene was estimated through Dahlberg's formula, which uses the frequencies of first cousin marriages among the patients' parents and in the general population were obtained from the collection of Catholic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::The radiation chemistry of the oligomer d(TpApCpG) X-irradiated in aqueous solution containing glutathione was studied. Four products were isolated by HPLC and characterized by NMR spectroscopy. Two of the major products are isomers of a 5-hydroxy-5,6-dihydrothymine modification of d(TpApCpG). A dihydrothymine modific...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014552141
更新日期:1990-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110069119
更新日期:2001-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802241754
更新日期:2008-08-01 00:00:00
abstract:PURPOSE:The tandemly repeated hexamers (TTAGGG)n present at the telomeric ends protect the human genome from a variety of environmental exposures including ionizing radiation. In order to find out the effect of chronic low dose radiation exposure, we have determined telomere length among newborns from high level natura...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.699135
更新日期:2012-09-01 00:00:00
abstract::The rate of recovery from radiation damage, as a function of dose per fraction, was investigated in mouse skin. Two different experimental designs were used, both incorporating the neutron top-up technique which enables the X-ray dose per fraction to be kept constant whilst changing the interfraction interval. Either ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550461
更新日期:1991-02-01 00:00:00
abstract:PURPOSE:The hemorrhagic syndrome is a major cause of morbidity and mortality associated with the acute radiation syndrome (ARS). We previously characterized the dose-response relationship for total body irradiation (TBI)-induced ARS in the New Zealand White (NZW) rabbit. Thrombocytopenia, hemorrhage, and anemia were st...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1820601
更新日期:2020-10-16 00:00:00
abstract:PURPOSE:Human embryonic stem cells (hESC) hold a great potential for regenerative medicine because, in principle, they can differentiate into any cell type found in the human body. In addition, studying the effect of ionizing radiation (IR) on hESC may provide valuable information about the response of human cells to I...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.700435
更新日期:2012-12-01 00:00:00
abstract:PURPOSE:Cells derived from individuals in which the ataxia telangiectasia (ATM) gene is mutated are hypersensitive to ionizing radiation. Whether differences in ATM protein levels exist among human malignant glioma cell lines and whether such differences are correlated with cellular radiosensitivity were determined. M...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141591
更新日期:1998-08-01 00:00:00
abstract:PURPOSE:To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells. MATERIALS AND METHODS:Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000410001723866
更新日期:2004-07-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414551441
更新日期:1994-11-01 00:00:00
abstract::The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551951
更新日期:1990-10-01 00:00:00
abstract:PURPOSE:To evaluate the radioprotective activity of C3, a regioisomer of water-soluble carboxyfullerene and a potent free radical scavenger, on both normal and tumour cells. MATERIALS AND METHODS:The murine committed bone-marrow stem cells for both granulocytes and monocytes (GM-CFC) were used to represent normal cell...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530001750068966
更新日期:2001-02-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract:PURPOSE:We already reported that levels of interferon (IFN)-γ have been shown to be markedly reduced in mice seven weeks after irradiation, resulting in a T helper (Th) 1/Th2 imbalance. To investigate whether the single or fractionated γ-irradiation induced an immune imbalance, we analysed the Th1-related immune respon...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.542540
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:The nature of the transferrable factor which goes from irradiated objects to bystander objects remains undefined. Most agree that a chemical entity is the likely 'factor' although some authors have produced in vitro evidence for the involvement of a physical component or a very potent volatile capable of travel...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.698366
更新日期:2012-08-01 00:00:00
abstract:PURPOSE:The low dose (<1 Gy) survival curve of mammalian cells is characterized by a region of hypersensitivity (HRS) followed by increased resistance (IRR). Above 1 Gy, the survival response can be described with a smooth downward-bending curve. Indirect evidence has indicated that the IRR response might reflect an in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138646
更新日期:2000-03-01 00:00:00
abstract::Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0.1...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550791
更新日期:1995-06-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139944
更新日期:1999-07-01 00:00:00