Abstract：
PURPOSE:The deleterious processes triggered by heavy ions on DNA were studied through the determination of the yield of a series of oxidized bases. Emphasis was placed on the estimation of the respective contribution of direct ionization and indirect effects, mostly by comparison with low linear energy transfer (LET) gamma-rays. MATERIAL AND METHODS:DNA samples and human monocytes were exposed either to gamma-rays emitted by a (60)Co source or to (12)C(6+) or (36)Ar(18+) ions. The levels of thymidine and 2'-deoxyguanosine oxidation products were determined by liquid chromatography coupled to tandem mass spectrometry subsequently to DNA digestion into nucleosides. RESULTS:The yields of thymidine lesions were similar to those of 8-oxo-7,8-dihydro-2'-deoxyguanosine within isolated DNA exposed either to gamma-rays or argon ions. Addition of spermine and Tris aimed at minimizing the indirect effect modified this ratio to the same extent with both types of radiation. In cells, the level of radiation-induced base damage was found to be correlated with the radiolytic yield of degrees OH that depends on the LET of the particle. In addition, radiation-induced thymidine and 2'-deoxyguanosine lesions were produced in similar amounts. In contrast, oxidation of 2'-deoxyguanosine was the main process when ionization was triggered in cellular DNA by ultraviolet laser-induced biphotonic processes. CONCLUSIONS:Predominant oxidation of 2'-deoxyguanosine is expected to be the hallmark of direct DNA ionization. The observation that thymidine and 2'-deoxyguanosine are equally damaged rules out a major contribution of the direct ionization in radiation-induced base damage to both isolated and cellular DNA by heavy ions. Dependence of the yield of lesions on the LET provides further support for this conclusion.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Douki T,Ravanat JL,Pouget JP,Testard I,Cadet Jdoi
10.1080/09553000600573788subject
Has Abstractpub_date
2006-02-01 00:00:00pages
119-27issue
2eissn
0955-3002issn
1362-3095pii
K130G4040J626331journal_volume
82pub_type
杂志文章abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552611
更新日期：1990-03-01 00:00:00
abstract：PURPOSE:This review will examine the role of phosphatases in cancer cell signalling and also outline emerging findings regarding the influence of phosphatases on tumor cell survival after ionising radiation. CONCLUSION:The exposure of tumour cells to clinically relevant doses of ionising radiation causes DNA damage an...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553000903232827
更新日期：2009-11-01 00:00:00
abstract：:The dose-rate effect has been the topic of extensive radiobiological studies and has important implications in radiation therapy and in the field of radiation protection. Three examples will be discussed: two in radiation therapy and one in protection. First, continuous low dose-rate interstitial brachytherapy may be ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009114550531
更新日期：1991-03-01 00:00:00
abstract：PURPOSE:Static magnetic field (SMF) exposure was shown experimentally to beneficially affect the healing process. The aim of this randomized, controlled trial was to evaluate the pain-inhibitory effect of local exposure to SMF on temporomandibular disorders, on alveolitis and on aphta in the oral mucosa. MATERIALS AND...
journal_title：International journal of radiation biology
pub_type： 杂志文章,随机对照试验
doi：10.3109/09553002.2012.661916
更新日期：2012-05-01 00:00:00
abstract：PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2017.1328750
更新日期：2017-10-01 00:00:00
abstract：:Radiation-resistant L5178Y-R (LY-R) and radiation-sensitive L5178Y-S (LY-S) murine lymphoma cells were X-irradiated and their free Ca2+ concentration was examined with Fura-2 in Ca2(+)-free salt solution. The release of free Ca2+ from intracellular stores was linear between 10 and 60 min after irradiation (1-5 Gy X-ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551481
更新日期：1990-07-01 00:00:00
abstract：PURPOSE:To construct a quantitative model of the radiation-induced bystander effect based on diffusion-type spreading of bystander signal communication between the hit and non-hit cells. Cell inactivation and induced oncogenic transformation by broad- and microbeam irradiation systems are considered. MATERIALS AND MET...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Recently, it has been demonstrated that two different thermal resistant states found in Chinese hamster cells, one transient, associated with thermotolerance, and the other permanent, associated with the increased expression of the cognate member of the hsp 70 family, are characterized by faster recovery from heat-ind...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314450751
更新日期：1993-05-01 00:00:00
abstract：:Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114552351
更新日期：1991-09-01 00:00:00
abstract：PURPOSE:To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. MATERIALS AND METHODS:Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20 h ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139700
更新日期：1999-08-01 00:00:00
abstract：PURPOSE:To develop and validate non-fluorescent chromosome painting for bright-field microscopy using the peroxidase/diaminobenzidine (DAB) reaction. MATERIALS AND METHODS:Peripheral blood lymphocytes were taken from patients with uterine cancer who had received heavy-ion radiation therapy. Chromosome slides were trea...
journal_title：International journal of radiation biology
pub_type：
doi：10.1080/095530098142077
更新日期：1998-05-01 00:00:00
abstract：PURPOSE:To investigate the effect of gamma radiation-induced alterations in antioxidant defence of radioresistant Sf9 insect cells. MATERIALS AND METHODS:Sf9 cells were irradiated at doses ranging from 0.5-200 Gy. Lipid peroxidation and protein carbonylation levels were observed at 4 h post-exposure along with reduced...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1054958
更新日期：2015-01-01 00:00:00
abstract：PURPOSE:To assess the possibility that extremely low frequency (ELF) magnetic fields obstruct the damage repair process, the gene conversion frequency and cell cycle kinetics in a DNA repair-proficient and nucleotide excision repair (NER)-deficient strain of diploid yeast Saccharomyces cerevisiae. MATERIALS AND METHOD...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001621437
更新日期：2003-11-01 00:00:00
abstract：PURPOSE:To illustrate how probability-generating functions (PGFs) can be employed to derive a simple probabilistic model for clonogenic survival after exposure to ionizing irradiation. METHODS:Both repairable and irreparable radiation damage to DNA were assumed to occur by independent (Poisson) processes, at intensiti...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138600
更新日期：2000-04-01 00:00:00
abstract：:The aim was to identify the decorporation and anti-oxidation efficacy of prompt and delayed consecutive administration of the catecholicpolyaminopolycarboxylate ligands 9501 and 7601 for radiothorium in vivo. The chelating agents 9501 or 7601 were administered intramuscularly to ICR mice 3 min or 3 days after intraper...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500141124
更新日期：2005-04-01 00:00:00
abstract：:Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550411
更新日期：1993-03-01 00:00:00
abstract：PURPOSE:To examine the relationship between cell killing and residual chromatin breaks after irradiation with qualitatively different types of radiation in six human cell lines. MATERIALS AND METHODS:Six human tumour cell lines and normal human cells were irradiated with 200 kV X-rays and two carbon-ion beams accelera...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134429
更新日期：2000-09-01 00:00:00
abstract：PURPOSE:To calculate the depth distribution of radioactive decays in bone by fitting a calculated alpha-particle energy spectrum to a measured spectrum. The dose to bone surface cells is then calculated from the depth distribution. MATERIALS AND METHODS:The spectra are calculated using Monte Carlo methods and a numeri...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098142734
更新日期：1998-01-01 00:00:00
abstract：:Intact Chinese hamster ovary cells were exposed to the restriction endonucleases (REs) AluI or BamHI. In metaphase spreads from these cells, 300 breakpoints per RE were localized in G-banded chromosome type aberrations (dicentrics, translocations, rings, terminal and interstitial deletions). The majority of breakpoint...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551401
更新日期：1995-10-01 00:00:00
abstract：:This paper presents a summary of our understanding to date of the formation of DNA strand breaks induced by highly energetic particle beams (high-LET radiation). We have compared our own recent data on the formation of strand breaks induced in DNA in an aqueous solution with our previous data and those of others avail...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014551431
更新日期：1990-07-01 00:00:00
abstract：:A novel 2-nitroimidazole with a theophylline side-chain, 7-(4'-(2-nitroimidazol-1-yl)-butyl)-theophylline, (NITP) was as efficient a hypoxic-cell radiosensitizer as misonidazole. However, if cells were irradiated with NITP under hypoxic conditions and then exposed to the drug under aerobic conditions, a much larger ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551671
更新日期：1992-06-01 00:00:00
abstract：:Because of the role that alveolar macrophages (PAM) play in the pulmonary clearance of inhaled particles via mechanical transport and dissolution, understanding the uptake and dissolution of particles by these cells might provide insight into the mechanisms of particle dissolution in lungs of various species and hence...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551861
更新日期：1992-07-01 00:00:00
abstract：PURPOSE:The purpose of this study was to assess the long-term effects of gamma-radiation, including low-dose radiation, on growth parameters of onion (Allium cepa) seedling roots 6-10 days after irradiation. MATERIALS AND METHODS:Onion seedlings were exposed to a 137Cs gamma source at doses ranging from 0.1 to 10 gray...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1844337
更新日期：2020-11-16 00:00:00
abstract：:Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1589013
更新日期：2019-10-01 00:00:00
abstract：PURPOSE:To evaluate the effects of 50 Hz magnetic fields (MF) on the development of cancer induced by ionizing radiation. MATERIALS AND METHODS:A total of 150 female CBA/S mice were randomized into three equal groups at the age of 3-5 weeks. One of the groups served as a 'cage-control group'. The two other groups were...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000010024696
更新日期：2001-04-01 00:00:00
abstract：:Purpose: Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1625462
更新日期：2019-09-01 00:00:00
abstract：PURPOSE:Epidemiological studies have demonstrated a possible correlation between exposure to extremely low-frequency magnetic fields (ELF-MF) and cancer. However, this correlation has yet to be definitively confirmed by epidemiological studies. The principal objective of this study was to assess the effects of 60 Hz ma...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.652724
更新日期：2012-04-01 00:00:00
abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000801998875
更新日期：2008-05-01 00:00:00
abstract：PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.518204
更新日期：2011-02-01 00:00:00
abstract：:The rate of recovery from radiation damage, as a function of dose per fraction, was investigated in mouse skin. Two different experimental designs were used, both incorporating the neutron top-up technique which enables the X-ray dose per fraction to be kept constant whilst changing the interfraction interval. Either ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550461
更新日期：1991-02-01 00:00:00
