The formation of strand breaks in DNA after high-LET irradiation: a comparison of data from in vitro and cellular systems.

abstract：:Only a small percentage of the DNA damage set by ionizing radiation in the living cell manifests itself as lethal. It is now increasingly accepted that clustered lesions may constitute the kind of damage that the repair enzymes cannot adequately deal with. The question is raised as to whether damage amplification reac...

journal_title：International journal of radiation biology

authors： Von Sonntag C

更新日期：1994-11-01 00:00:00

abstract：:Mouse C3H 10T1/2 cells are most sensitive to radiation-induced neoplastic transformation in the G2/M-phase of the cell cycle. When synchronized 10T1/2 cells were exposed to phorbol 12-myristate 13-acetate (TPA) after irradiation, transformation of cells not in the transformation-sensitive window was enhanced, but tran...

journal_title：International journal of radiation biology

authors： Wells RL,Cao J,Xing Y,He L,Elkind MM

更新日期：1993-12-01 00:00:00

abstract：PURPOSE:The low dose (<1 Gy) survival curve of mammalian cells is characterized by a region of hypersensitivity (HRS) followed by increased resistance (IRR). Above 1 Gy, the survival response can be described with a smooth downward-bending curve. Indirect evidence has indicated that the IRR response might reflect an in...

journal_title：International journal of radiation biology

authors： Marples B,Joiner MC

更新日期：2000-03-01 00:00:00

abstract：PURPOSE:The aim of this work is to study the relative damaging effects of DNA-incorporated radionuclides by analyzing the behavior of the liquid-scintillation counting efficiency in volumes of nanometer size. METHODS:A liquid scintillation counter can detect changes in the micelle size when different percentages of an...

journal_title：International journal of radiation biology

authors： Grau Carles A

更新日期：2008-12-01 00:00:00

abstract：PURPOSE:When Iodine-125 decays within chromatin, several in vivo experiments have shown that the radiobiological effects are caused mainly by indirect mechanisms and that more than one DNA double strand break (DSB) is produced per decay. We present calculations to evaluate the contribution of direct and indirect effect...

journal_title：International journal of radiation biology

authors： Terrissol M,Edel S,Pomplun E

更新日期：2004-11-01 00:00:00

abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...

journal_title：International journal of radiation biology

authors： Tretter L,Rónai E,Szabados G,Hermann R,Andó A,Horváth I

更新日期：1990-03-01 00:00:00

abstract：:Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...

journal_title：International journal of radiation biology

authors： Cai WB,Roberts SA,Bowley E,Hendry JH,Potten CS

更新日期：1997-02-01 00:00:00

abstract：:The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of in...

journal_title：International journal of radiation biology

authors： Chetioui A,Despiney I,Guiraud L,Adoui L,Sabatier L,Dutrillaux B

更新日期：1994-05-01 00:00:00

abstract：:Cell killing and the production of DNA double-strand breaks (dsbs) were measured in parallel for V79 cells and a human tumour cell line (Caski) following exposure to radiations of differing linear energy transfer (LET) including accelerated heavy ions. Dsb induction was assessed by neutral filter elution (pH 7.4) and ...

journal_title：International journal of radiation biology

authors： Weber KJ,Flentje M

更新日期：1993-08-01 00:00:00

abstract：PURPOSE:Angiotensin-converting enzyme (ACE) inhibitors are effective in the prophylaxis of radiation-induced renal and lung injury. Studies were designed to determine whether blocking the angiotensin II (AII) receptor, rather than blocking AII synthesis with ACE inhibitors, would also be effective. MATERIALS AND METHO...

journal_title：International journal of radiation biology

authors： Moulder JE,Fish BL,Cohen EP

更新日期：1998-04-01 00:00:00

abstract：PURPOSE:To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells. MATERIALS AND METHODS:Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasin...

journal_title：International journal of radiation biology

authors： Dhillon VS,Thomas P,Fenech M

更新日期：2004-07-01 00:00:00

abstract：:Cells use substrates such as glucose and glutamine to provide energy for repair of radiation damage. Glutaminolysis and glycolysis were inhibited by aminooxyacetic acid (AOAA) and 2-deoxyglucose (2DG), respectively, to inhibit metabolism of these substrates in order to determine the effect on radiation response of CHO...

journal_title：International journal of radiation biology

authors： Hunter AJ,Blekkenhorst GH

更新日期：1998-03-01 00:00:00

abstract：:BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...

journal_title：International journal of radiation biology

authors： van den Aardweg GJ,Hopewell JW,Adams GE,Barnes DW,Sansom JM,Stratford IJ,Nethersell AB

更新日期：1991-04-01 00:00:00

abstract：:We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 d...

journal_title：International journal of radiation biology

authors： Okayasu R,Iliakis G

更新日期：1992-04-01 00:00:00

abstract：PURPOSE:The behavioral responses of planaria to the exposures of a range of concentrations of morphine (μM to attoM) or the μ-opiate antagonist naloxone or to either of these compounds and a burst-firing magnetic field (5 μT) were studied. MATERIAL AND METHODS:The locomotor velocity (LMV) of planaria was measured afte...

journal_title：International journal of radiation biology

authors： Murugan NJ,Persinger MA

更新日期：2014-10-01 00:00:00

abstract：PURPOSE:To evaluate the impact of oral administration of purslane (Portulaca oleracea) extract or fish oil and their co-treatments in the modulation of radiation-induced damage. MATERIAL AND METHODS:Purslane (P) (400 mg/kg body weight) or fish oil (Fo) (60 mg/kg body weight) was administrated to male albino rats via g...

journal_title：International journal of radiation biology

authors： Abd El-Azime AS,Hussein EM,Ashry OM

更新日期：2014-12-01 00:00:00

abstract：:The relative efficiencies of two types of densely ionizing particles were compared for the induction of mutations at two distinct genetic loci in human cells. Mutations to 6-thioguanine resistance (hgprt locus) or to trifluorothymidine resistance (tk) locus were scored in TK6 human lymphoblastoid cells exposed to grad...

journal_title：International journal of radiation biology

authors： Kronenberg A,Little JB

更新日期：1989-06-01 00:00:00

abstract：PURPOSE:The purpose of this study was to assess the long-term effects of gamma-radiation, including low-dose radiation, on growth parameters of onion (Allium cepa) seedling roots 6-10 days after irradiation. MATERIALS AND METHODS:Onion seedlings were exposed to a 137Cs gamma source at doses ranging from 0.1 to 10 gray...

journal_title：International journal of radiation biology

authors： Bolsunovsky A,Trofimova E,Dementyev D,Petrichenkov M

更新日期：2020-11-16 00:00:00

abstract：:One of the major goals of cancer research is to identify and understand the causes of cellular proliferation. The role of cell death, or lack thereof, in carcinogenesis, tumour growth, metastatic spread and response to treatment has been largely overlooked even though the morphology of apoptosis (programmed cell death...

journal_title：International journal of radiation biology

authors： Blank KR,Rudoltz MS,Kao GD,Muschel RJ,McKenna WG

更新日期：1997-05-01 00:00:00

abstract：PURPOSE:The purpose of this work was to determine whether the cellular components of Hairless-rat skin are affected by a chronic local exposure to non-ionizing radiations of Global Mobile Phone System: GSM-900 or -1800 radiations at specific absorption rate (SAR) 2.5 and 5 W/kg. MATERIALS AND METHODS:A selected part o...

journal_title：International journal of radiation biology

authors： Sanchez S,Masuda H,Billaudel B,Haro E,Anane R,Lévêque P,Ruffie G,Lagroye I,Veyret B

更新日期：2006-09-01 00:00:00

abstract：PURPOSE:As the most recent epidemiological studies provide no definite conclusions about the effects of 50/60 Hz magnetic fields (MFs) on the incidence of leukaemia in humans, animal models in a well-controlled environment are useful for evaluating the possibility of an association between MFs and leukaemia. The presen...

journal_title：International journal of radiation biology

authors： Devevey L,Patinot C,Debray M,Thierry D,Brugere H,Lambrozo J,Guillosson JJ,Nafziger J

更新日期：2000-06-01 00:00:00

abstract：PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...

journal_title：International journal of radiation biology

authors： Krause M,Schmitz M,Noessner E,Skrablin PS,Wehner R,Rieber EP,Baumann M

更新日期：2007-11-01 00:00:00

abstract：:The effect of heat (43-45 degrees C) on the induction and the repair of DNA double-strand breaks (dsb) was studied in CHO cells after 60 Gy of X-rays using constant-field gel electrophoresis. Heat given prior to irradiation was found not to alter the number of dsb when measured immediately after irradiation. In non-he...

journal_title：International journal of radiation biology

authors： Dahm-Daphi J,Brammer I,Dikomey E

更新日期：1997-08-01 00:00:00

abstract：PURPOSE:We aimed for a quantitative evaluation that justifies guidelines for evacuation which take into consideration both the human and economic costs. To the best of our knowledge, such an evaluation has not been performed yet. The present guidelines published by the International Atomic Energy Agency (IAEA) are prob...

journal_title：International journal of radiation biology

authors： Yanovskiy M,Levi ON,Shaki YY,Socol Y

更新日期：2020-11-01 00:00:00

abstract：:We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dode...

journal_title：International journal of radiation biology

authors： Müller WU,Bauch T,Streffer C,Niedereichholz F,Böcker W

更新日期：1994-03-01 00:00:00

abstract：:Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...

journal_title：International journal of radiation biology

authors： Nese C,Yuan Z,Schuchmann MN,Von Sonntag C

更新日期：1992-11-01 00:00:00

abstract：:Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...

journal_title：International journal of radiation biology

authors： Jung T,Streffer C

更新日期：1991-09-01 00:00:00

abstract：:During previous studies on the relationship between thermosensitivity and intracellular pH (pHi) regulation in mammalian cells, we observed that the thermoresistant TR-4 cells appeared to be resistant to manipulations of the Na+/H+ antiport, one of the two primary membrane regulators of pHi. We hypothesized that this ...

journal_title：International journal of radiation biology

authors： Liu FF,Lee C,Li JH

更新日期：1996-10-01 00:00:00

abstract：PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...

journal_title：International journal of radiation biology

authors： Liamsuwan T,Uehara S,Emfietzoglou D,Nikjoo H

更新日期：2011-02-01 00:00:00

abstract：:An assay system was developed to detect changes of restriction fragment profiles obtained after pulsed-field gel electrophoresis (PFGE) for DNA from mammalian cells that were irradiated and incubated for repair. DNA was prepared from irradiated log-phase human melanoma cells (MRI 221) after incubation for repair (6 h)...

journal_title：International journal of radiation biology

authors： Latz D,Dewey WC,Flentje M,Lohr F,Wenz F,Weber KJ

更新日期：1996-12-01 00:00:00