Abstract:
:We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 degrees C during lysis with sodium-N-laurylsarcosine (NLS) resulting in almost linear dose-response curves. We also examined chromatin decondensation as a contributing factor in the linearization observed in the elution dose-response curve under the above conditions. When nuclei with condensed chromatin were prepared from irradiated cells, applied on the filter and lysed with NLS at room temperature, a shoulder-type elution dose-response curve was obtained only slightly higher than that of cells lysed under the same conditions. However, when nuclei prepared from irradiated cells were applied on the filter after relaxation of chromatin by incubation in low ionic strength buffer and lysed with NLS at room temperature, an almost linear dose-response curve was obtained similar to that of cells lysed with NLS at 60 degrees C. Lysis with NLS at 60 degrees C of nuclei with relaxed chromatin did not further modify the DNA elution dose-response curve. Based on these results we propose that the linearization of the DNA elution dose-response curve observed after chromatin decondensation reflects a reduction in the degree of chromatin compactness in the nuclear complexes that leads to a relatively uniform distribution of the DNA on the filter and reduces trapping of elutable material in the compact nuclear structures otherwise present. Since high radiation doses dissolve compact nuclear structures, trapping of elutable material is expected to be highest at low doses of radiation, leading to the observed reduction in the fraction of DNA eluted per Gy at low versus high radiation doses and thus to the observed shoulder. Furthermore, we propose that the linearization of the DNA elution dose-response curves observed in cells lysed in NLS at 60 degrees C may also be due to a decondensation of the nuclear complexes on the filter as a result of the combined action of detergent and high temperature. The notion of a correlation between DNA elution dose response and cell radiosensitivity was examined in two human (SQ20B, SCC61) and two Chinese hamster (V-79, irs-2) cell lines with widely different radiosensitivities.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Okayasu R,Iliakis Gdoi
10.1080/09553009214551211subject
Has Abstractpub_date
1992-04-01 00:00:00pages
455-63issue
4eissn
0955-3002issn
1362-3095pii
L0GU2X35Y6BFK4LLjournal_volume
61pub_type
杂志文章abstract:PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1328750
更新日期:2017-10-01 00:00:00
abstract::Purpose: A study is presented of the irradiation of cancerous cervical cell line HeLa loaded with a platinum salt, betamethasone and deoxyglucose. The presence of the platinum increases the free-radical concentration and augments the cell death rate, whereas betamethasone or deoxyglucose induces radiosensitization by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1721594
更新日期:2020-05-01 00:00:00
abstract:PURPOSE:To propose an improved version of RADACK, a stochastic simulation of radiolytic attack on DNA, that takes into account the reactivity of each amino acid of a specifically bound protein with hydroxyl radicals. To apply it to the natural lactose operator-repressor complex taking advantage of recently reported str...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000137788
更新日期:2003-06-01 00:00:00
abstract::Photoirradiation of aqueous solutions of DNA in the presence of Al- or Zn-tetrasulphonated phthalocyanines (AIPcS4 and ZnPcS4) causes formation of strand breaks and liberation of nucleobases. The effect of added D2O, which enhances singlet oxygen (1O2) lifetime, radical scavengers including alcohols and the spin-trap ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::Existing models of chromosome aberrations produced by ionizing-radiation predict equal numbers of dicentrics and translocations if the dose is so low that complex aberrations can be ignored. We show that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at highe...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145977
更新日期:1996-02-01 00:00:00
abstract:PURPOSE:To determine whether cigarette smoke condensate (CSC) without metabolic activation induces direct DNA double strand breaks (DSB) in the G1 phase of various radiosensitive mutants of CHO cells and whether these breaks display collateral hypersensitivity to CSC with respect to cell killing. MATERIALS & METHODS:W...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701481790
更新日期:2007-09-01 00:00:00
abstract:PURPOSE:To determine the radiosensitivity difference of human Cluster of Differentiation (CD)4(+)CD25(+) regulatory T cells (Treg) and effector T cells to low dose gamma ray and elucidate the underlying mechanisms in vitro. MATERIALS AND METHODS:Blood samples were collected from five health subjects and five patients ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518208
更新日期:2011-01-01 00:00:00
abstract::The effect of gamma-radiation on aqueous solutions of saturated phospholipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine (lysoPC), and bovine brain sphingomyelin (SM) has been investigated. It is shown that th...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143888
更新日期:1997-05-01 00:00:00
abstract:PURPOSE:The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of thi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1427903
更新日期:2018-03-01 00:00:00
abstract:PURPOSE:A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epitheli...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530098141023
更新日期:1998-12-01 00:00:00
abstract::Primary chick embryo myoblasts can be a useful tool for studying the developmental events which accompany myoblast differentiation, particularly myoblast membrane fusion. To determine whether the electrical properties and/or fusion in these systems are affected by 50 Hz magnetic fields, chick embryo myoblast cultures ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract:PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110034649
更新日期:2001-05-01 00:00:00
abstract:PURPOSE:Radiation-induced bystander effects have important implications in radiotherapy. Their persistence in normal cells may contribute to risk of health hazards, including cancer. This study investigates the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of harmful e...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.950715
更新日期:2015-01-01 00:00:00
abstract::Survival of HT29 cells was measured after irradiation with single doses of X-rays (0.05-5 Gy) and neutrons (0.025-1.5 Gy), using a Dynamic Microscopic Imaging Processing Scanner (DMIPS) with which individual cells can be accurately located in tissue culture flasks, their positions recorded, and after an appropriate in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314450831
更新日期:1993-05-01 00:00:00
abstract::Sample preparation procedures for the pulsed-field gel electrophoresis (PFGE) assay usually involve a lysis step at temperatures as high as 50 degrees C. During this warm-lysis procedure, multiply damaged sites containing heat-labile sites (HLS) can be converted into double-strand breaks (DSB). Once formed, these DSB ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500156577
更新日期:2005-06-01 00:00:00
abstract:PURPOSE:The effect of boronophenylalanine (BPA)-mediated boron neutron capture therapy (BNCT) on human oral squamous cell carcinoma (SCC) xenografts in nude mice was examined. MATERIALS AND METHODS:Tumor-bearing mice were given BPA at a dose of 250 mg/kg body weight. The tumor (10)B concentration 2 h after an injectio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600570453
更新日期:2006-01-01 00:00:00
abstract::One-cell mouse embryos were irradiated with X-rays or neutrons. Analysis of the first, second, and third postradiation mitoses revealed that the yields of structural aberrations increased linearly after exposure to both radiation qualities. For X-rays the aberration frequency decreased from the first to the third mito...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551771
更新日期:1988-09-01 00:00:00
abstract::We used three common fluorescent probes to measure pericentric inversion frequencies in 2.9 Gy 60Co gamma-irradiated human lymphocytes. For a given chromosome, the first probe is specific to one telomeric region, the second probe is specific to one subcentromeric region and the third probe is specific to the centromer...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097144382
更新日期:1997-01-01 00:00:00
abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551821
更新日期:1991-07-01 00:00:00
abstract:PURPOSE:Zearalenone is a potent estrogenic metabolite produced by some Fusarium and Gibberella species. Therefore, the aim of this study was to determine the accumulation of Technetium-[99m]-labeled zearalenone in mice organs as well as to study the protective effect of Lactobacillus plantarum. MATERIALS AND METHODS:T...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1524990
更新日期:2018-12-01 00:00:00
abstract::In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144860
更新日期:1996-10-01 00:00:00
abstract:PURPOSE:To investigate whether radiation survival of cells irradiated aerobically in the oxygen-sensitive restriction point in late G1 is dependent on where in the cell cycle the cells first were rendered hypoxic. MATERIALS AND METHODS:Human cervix carcinoma, NHIK 3025 cells, were synchronized and rendered hypoxic whi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141366
更新日期:1998-10-01 00:00:00
abstract::Purpose To investigate the effect of increasing the overall treatment time as well as delivering the compensating doses on the Balb/c breast adenocarcinoma (4T1) tumor. Materials and methods A total of 72 mice were divided into two aliquots (classes A and B) based on the initial size of their induced tumor. Each class...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2016.1117677
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:To analyze the direct and transgenerational effects of exposure to low-dose 1 GHz (mobile phone/wireless telecommunication range) and 10 GHz (radar/satellite communication range) radiofrequency electromagnetic fields (RF-EMF) on the motility of ciliates Spirostomum ambiguum. MATERIALS AND METHODS:S. ambiguum w...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.868612
更新日期:2014-03-01 00:00:00
abstract:PURPOSE:Exposure to ionizing radiation induces plant defenses by regulating the expression of response genes. The systemic acquired resistance deficient 1 (SARD1) is a key gene in plant defense response. In this study, the function of Oryza sativa SARD1 (OsSARD1) was investigated after exposure of seeds/plants to ioniz...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1297901
更新日期:2017-07-01 00:00:00
abstract::The past recommendations of the ICRP about dose limits to the skin are reviewed. Recently, an ICRP Task Group has been revisiting the old arguments and setting them against new data. With the exception of the function of cells in the skin associated with immunocompetence, non-stochastic effects have been well characte...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014550961
更新日期:1990-04-01 00:00:00
abstract::Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550411
更新日期:1993-03-01 00:00:00
abstract:PURPOSE:To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. MATERIALS AND METHODS:Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.556175
更新日期:2011-05-01 00:00:00
abstract::A new approach is reported that combines synchrotron radiolysis and mass spectrometry to probe the structure of proteins. Hydroxyl radicals produced upon the radiolysis of protein solutions using synchrotron light modify amino acid side-chains on millisecond timescales. This results in the formation of stable oxidatio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110094805
更新日期:2002-02-01 00:00:00
abstract:PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701317366
更新日期:2007-06-01 00:00:00