Abstract：
:Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assay in the large bowel. Various times post-irradiation were studied using different approaches and threshold criteria for surviving crypts. Small, but statistically significant, differences were observed along the length of the small intestine, but no differences were observed between different regions of the large intestine. There was a marked difference (p < 0.001) in the response in the large compared with the small intestine (D0 = 291 +/- 14 compared with 151 +/- 4 cGy respectively with corresponding extrapolation numbers of 18 +/- 4 and 377 +/- 65). The use of tritiated thymidine or vincristine helped in the identification of true survivors in the large bowel, but these approaches are not necessary in the small bowel. The longer the time after irradiation that the samples were fixed the easier it was to identify survivors in the colon. However, the longer the time the more animals died. The optimum compromise in these studies was to use partial-body irradiation (abdomen only) and to sample on the fifth day after irradiation. With these criteria the mid-colon survival curve had a D0 = 274 +/- 28 cGy and an extrapolation number of 22 +/- 10. The results can be considered in relation to published data on the levels of p53 and bcl-2 expression and apoptosis in murine small and large bowel.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Cai WB,Roberts SA,Bowley E,Hendry JH,Potten CSdoi
10.1080/095530097144265subject
Has Abstractpub_date
1997-02-01 00:00:00pages
145-55issue
2eissn
0955-3002issn
1362-3095journal_volume
71pub_type
杂志文章abstract：:Bacterial infection of simple wounds was studied directly and quantitatively in adult mice given 6.5 Gy 60Co. Three days later, when neutropenia was evident, the skin and the medial gluteus muscle of anaesthetized mice were incised. A suspension of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae or Stre...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551671
更新日期：1990-08-01 00:00:00
abstract：:The response to radiation of seven osteosarcoma cell lines was analysed by in vitro colony-forming assay and compared with that of eight human fibroblast strains. The values of D0, the surviving fraction after 2 Gy (S2Gy), and the mean inactivation dose (D) of osteosarcoma cells in log-phase culture were significantly...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214552351
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550361
更新日期：1991-02-01 00:00:00
abstract：:We studied the extent of split-dose recovery in seven non-transformed human fibroblast cell lines of different intrinsic radiosensitivity (HF19, 1BR3, 149BR, 84BR, GM739, 180BR and AT2EM). Experiments were performed on both growing and plateau-phase cells. The seven cell lines displayed a wide range of intrinsic radio...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096146075
更新日期：1996-02-01 00:00:00
abstract：PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...
journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139593
更新日期：1999-09-01 00:00:00
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journal_title：International journal of radiation biology
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更新日期：2008-02-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140221
更新日期：1999-05-01 00:00:00
abstract：PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110075220
更新日期：2001-12-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098142617
更新日期：1998-02-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.754556
更新日期：2013-04-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145779
更新日期：1996-04-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
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journal_title：International journal of radiation biology
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更新日期：2006-09-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
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更新日期：1997-05-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
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journal_title：International journal of radiation biology
pub_type： 杂志文章
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journal_title：International journal of radiation biology
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更新日期：1998-07-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
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更新日期：2011-10-01 00:00:00
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journal_title：International journal of radiation biology
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更新日期：2003-03-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551721
更新日期：1993-11-01 00:00:00
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journal_title：International journal of radiation biology
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更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2011-02-01 00:00:00
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journal_title：International journal of radiation biology
pub_type： 杂志文章
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journal_title：International journal of radiation biology
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更新日期：2020-11-12 00:00:00
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journal_title：International journal of radiation biology
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doi：10.3109/09553002.2014.911421
更新日期：2014-10-01 00:00:00
