Abstract：
PURPOSE:The effects of radiation +/- hypogravity on immunologic function were investigated using the Partial Weight Suspension (PWS) model ( Wagner et al. 2010 ). MATERIALS AND METHODS:Mice were exposed to 0.5, 1, or 2 Gray (Gy) dose of gamma radiation and then placed in the PWS system for 4, 24, 48 hours, or 4 days. Spleens were excised and white blood cells were prepared for flow cytometry analyses. RESULTS:The combination of PWS + radiation (1 and 2 Gy doses only) resulted in decreased cell viability at the 24 h (∼16% decrease), 48 h (∼20% decrease), and 4 day (∼20% decrease) time points, compared to the PWS (no radiation) and no treatment (non-suspended, non-irradiated) groups. The T lymphocyte (thymus-derived) population increased by ∼10% (24 h, 48 h, and 4 day time points), while the B lymphocyte (bursal or bone marrow-derived) population decreased by ∼10% (at all time points examined), when mice were exposed to PWS + radiation (2 Gy dose only), compared to the PWS or no treatment groups. T cell activation was observed in the PWS group and the 0.5 Gy +/- PWS groups at the 4 and 24 h time points, compared to the no treatment group. However, T cell activation was significantly suppressed (∼85%) at the acute time points in the 2 Gy +/- PWS groups, comparable to the no treatment group. CONCLUSIONS:Ionizing radiation in the absence and presence of simulated hypogravity results in acute lymphocyte dysfunction and compromised immune response.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Sanzari JK,Wilson JM,Wagner EB,Kennedy ARdoi
10.3109/09553002.2011.595875subject
Has Abstractpub_date
2011-10-01 00:00:00pages
1033-8issue
10eissn
0955-3002issn
1362-3095journal_volume
87pub_type
杂志文章abstract：PURPOSE:To investigate the potential total and relative free-radical transfer from histones to DNA in X-irradiated chromatin, by analysing the relative contributions of individual radicals from protein and from DNA. MATERIALS AND METHODS:Chromatin was isolated from calf thymus, freeze-dried and either used as such or ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050111541
更新日期：2000-08-01 00:00:00
abstract：:Radiation-resistant L5178Y-R (LY-R) and radiation-sensitive L5178Y-S (LY-S) murine lymphoma cells were X-irradiated and their free Ca2+ concentration was examined with Fura-2 in Ca2(+)-free salt solution. The release of free Ca2+ from intracellular stores was linear between 10 and 60 min after irradiation (1-5 Gy X-ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551481
更新日期：1990-07-01 00:00:00
abstract：:An assay system was developed to detect changes of restriction fragment profiles obtained after pulsed-field gel electrophoresis (PFGE) for DNA from mammalian cells that were irradiated and incubated for repair. DNA was prepared from irradiated log-phase human melanoma cells (MRI 221) after incubation for repair (6 h)...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144518
更新日期：1996-12-01 00:00:00
abstract：PURPOSE:To determine the relationships between the frequencies of radiation-induced chromosomal alterations and the extent of apoptosis in G0 human lymphocytes. MATERIAL AND METHODS:G0 human peripheral blood lymphocytes (HPBL) were X or gamma-irradiated, in the presence or absence of the repair inhibitor cytosine arab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500303690
更新日期：2005-08-01 00:00:00
abstract：:Chinese hamster ovary cells were exposed to various heat treatments followed by X-irradiation, and the induction and repair of DNA strand breaks was studied using the alkaline unwinding technique. Heat treatments alone were found to cause DNA strand breakage only for temperatures greater than or equal to 43 degrees C,...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214550851
更新日期：1992-02-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551051
更新日期：1995-08-01 00:00:00
abstract：PURPOSE:Since previous research found an increase in the rate of aneuploidies in human lymphocytes exposed to radiofrequencies, it seems important to perform further studies. The objective of this study was then to investigate whether the exposure to RF (radiofrequency) radiation similar to that emitted by mobile phone...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.542543
更新日期：2011-04-01 00:00:00
abstract：PURPOSE:The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1101500
更新日期：2016-01-01 00:00:00
abstract：PURPOSE:To carry out a comparative study on potential cytogenetic fingerprints for radiation LET in human metaphase lymphocytes. MATERIALS AND METHOD:Human lymphocytes were irradiated in vitro with 3.0 Gy 60Co gamma-rays, 0.9 Gy 3H beta-rays or 0.2 Gy 2.7 Mev neutrons. Detailed chromosome aberrations were analysed by ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050201073
更新日期：2000-12-01 00:00:00
abstract：PURPOSE:The present study was designed to reveal, whether the 30 min daily full body exposure of mice to an inhomogeneous static magnetic field (SMF) has a statistically significant effect on diabetic neuropathy. MATERIALS AND METHODS:Three different doses (100, 150, or 200 mg/kg) of streptozotocin (STZ) were administ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.518200
更新日期：2011-01-01 00:00:00
abstract：PURPOSE:Human diseases associated with acute radiation responses are rare genetic disorders with common clinical and biological features including radiosensitivity, genomic instability, chromosomal aberrations, and frequently immunodeficiency. To determine what molecular assays are predictive of cellular radiosensitivi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701797039
更新日期：2008-02-01 00:00:00
abstract：:Samples of human rib were collected at autopsy and 20 were selected for marrow fat cell measurement, representing an age range of 16-96 years. The mean diameter of fat cells in red bone marrow of human rib was found to increase from around 48 microns at ages 16-29 years to around 65 microns at ages 82-96 years. There ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551681
更新日期：1995-12-01 00:00:00
abstract：:In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In mos...
journal_title：International journal of radiation biology
pub_type：
doi：10.1080/095530097143220
更新日期：1997-10-01 00:00:00
abstract：PURPOSE:DNA damage is one of the main consequences of exposure to ionizing irradiation (IR). Recent studies indicate that IR can modulate the expression of immune system-related genes. However, the effects of IR on the expression of genes and pathways of the B7-CD28 superfamily remain poorly defined. The aim of this st...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1857454
更新日期：2020-12-17 00:00:00
abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552611
更新日期：1990-03-01 00:00:00
abstract：:The mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological do...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551321
更新日期：1994-10-01 00:00:00
abstract：PURPOSE:The deleterious processes triggered by heavy ions on DNA were studied through the determination of the yield of a series of oxidized bases. Emphasis was placed on the estimation of the respective contribution of direct ionization and indirect effects, mostly by comparison with low linear energy transfer (LET) g...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000600573788
更新日期：2006-02-01 00:00:00
abstract：PURPOSE:Ukrain, an alkaloid thiophosphoric acid derivative of Chelidonium majus L., has demonstrated a promising impact on chemotherapy in a variety of malignancies. The effects of the drug on cell survival, alteration of the cell cycle and induction of apoptosis were examined without and in combination with ionizing r...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110089991
更新日期：2002-01-01 00:00:00
abstract：:We used a cell sorter assay to evaluate the efficacy of the hypoxic cell sensitizer etanidazole over 3-4 logs of cell inactivation, with particular attention to the clinically relevant low-dose survival region. Analysis of the radiation responses in a panel of six human tumour cell lines under conditions of hypoxia an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144617
更新日期：1996-12-01 00:00:00
abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009414551661
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:The low dose (<1 Gy) survival curve of mammalian cells is characterized by a region of hypersensitivity (HRS) followed by increased resistance (IRR). Above 1 Gy, the survival response can be described with a smooth downward-bending curve. Indirect evidence has indicated that the IRR response might reflect an in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138646
更新日期：2000-03-01 00:00:00
abstract：PURPOSE:To examine how folate status influences chromosomal damage following X-ray irradiation. MATERIAL AND METHODS:In an animal study, mice were fed either a low, basal, or high folic acid diet (0, 2, or 40 mg/kg diet, respectively) for 4 weeks, and then given total body irradiation (TBI) at 0.5 Gy. In a human study...
journal_title：International journal of radiation biology
pub_type： 杂志文章,随机对照试验
doi：10.1080/09553000600721817
更新日期：2006-04-01 00:00:00
abstract：:BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550921
更新日期：1991-04-01 00:00:00
abstract：PURPOSE:To investigate the induction and persistence of chromosome aberrations in mouse bone marrow cells after X-ray exposure and to detect differential involvement of individual chromosomes in translocations. MATERIALS AND METHODS:Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome aberration...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139593
更新日期：1999-09-01 00:00:00
abstract：PURPOSE:To investigate the mechanism of methylene blue-induced radiosensitization in Escherichia coli cells. MATERIALS AND METHODS:Bacteriophage lambda15 was irradiated with X-rays in the presence or absence of methylene blue (MB) and infected into bacteria with different repair capabilities preincubated with or witho...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050134528
更新日期：2000-09-01 00:00:00
abstract：PURPOSE:The effect of boronophenylalanine (BPA)-mediated boron neutron capture therapy (BNCT) on human oral squamous cell carcinoma (SCC) xenografts in nude mice was examined. MATERIALS AND METHODS:Tumor-bearing mice were given BPA at a dose of 250 mg/kg body weight. The tumor (10)B concentration 2 h after an injectio...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000600570453
更新日期：2006-01-01 00:00:00
abstract：:CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551361
更新日期：1993-08-01 00:00:00
abstract：:We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dode...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550371
更新日期：1994-03-01 00:00:00
abstract：PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.901579
更新日期：2014-06-01 00:00:00
abstract：:We have examined the timing of gamma-irradiation-induced death in relation to cell cycle progression using a panel of mouse lymphoid or myeloid cell lines. Death was found to occur immediately after irradiation ('rapid interphase' death), or after arrest in G2 phase ('delayed interphase' death). In part II of this ser...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550261
更新日期：1994-02-01 00:00:00
