Abstract:
PURPOSE:To investigate the induction and persistence of chromosome aberrations in mouse bone marrow cells after X-ray exposure and to detect differential involvement of individual chromosomes in translocations. MATERIALS AND METHODS:Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome aberrations in bone marrow cells were analysed at 1, 7, 21 and 100 days following irradiation by means of fluorescence in situ hybridization (FISH) with mouse chromosome-specific DNA libraries (#1,13; #2,8; #6,15 and X,Y). In total, 38% of mouse genome was painted and examined. RESULTS:Pooled data indicate that the frequencies of dicentrics and fragments decreased with time and reached to the control level at day 21 after exposure. Following exposure to 1 Gy of X-rays, the frequencies of translocations were not significantly lower between days 7 and 100 than observed at day 1. However, the frequencies of translocations for the 3 Gy group were significantly (about 40%) lower at day 7, then remained constant up to day 100. After exposure to 3Gy of X-rays, the frequencies of non-reciprocal translocations decreased with time, whereas reciprocal translocations between days 7 and 100 were not significantly less frequent than at day 1. A comparison of observed and expected numbers of translocations involving individual chromosomes showed that at day 1 after irradiation, distribution of X-ray-induced translocations among the painted chromosomes was proportional to their DNA content. However, at day 100 after exposure, the observed translocations involving chromosome 2 were more frequent than expected, those involving chromosomes 8 and 15 were less frequent than expected, while chromosomes 1, 6, X and Y were involved as frequently as expected. CONCLUSION:Among induced translocations, non-reciprocal translocations are relatively unstable, especially after exposure to high-dose X-rays. While the initial distribution of X-ray-induced translocations is proportional among the painted chromosomes, the persistence of these translocations is heterogeneous.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Xiao Y,Darroudi F,Grigorova M,Natarajan ATdoi
10.1080/095530099139593subject
Has Abstractpub_date
1999-09-01 00:00:00pages
1119-28issue
9eissn
0955-3002issn
1362-3095journal_volume
75pub_type
杂志文章abstract:PURPOSE:A model that identifies radiation-induced genetic instability as the earliest cellular event in the multi-step sequence leading to radiation-induced cancer was previously proposed. In this paper ongoing experiments are discussed which are designed to test this model and its predictions in mouse mammary epitheli...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530098141023
更新日期:1998-12-01 00:00:00
abstract::We have measured clonal survival following gamma-irradiation of unstimulated (G0) T-lymphocytes from 35 donors, of 11 T-lymphocyte cell lines, of six lymphoblastoid cell lines, and of nine primary fibroblast strains for which we have G0 T-lymphocyte material from the same donor. Amongst the G0 lymphocytes we have resu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552331
更新日期:1988-12-01 00:00:00
abstract:PURPOSE:To investigate whether mobile phone radiation might affect snail nociception, employing radiofrequency (RF) electromagnetic fields (EMF) which, to our knowledge, have hitherto not been studied in a snail model. Exposure to extremely low frequency (ELF) magnetic fields has however been shown to significantly aff...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.644257
更新日期:2012-03-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300032000093146
更新日期:2003-05-01 00:00:00
abstract:PURPOSE:The nature of the transferrable factor which goes from irradiated objects to bystander objects remains undefined. Most agree that a chemical entity is the likely 'factor' although some authors have produced in vitro evidence for the involvement of a physical component or a very potent volatile capable of travel...
journal_title:International journal of radiation biology
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更新日期:2012-08-01 00:00:00
abstract:PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.809170
更新日期:2013-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:2006-09-01 00:00:00
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更新日期:1992-06-01 00:00:00
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更新日期:2013-04-01 00:00:00
abstract::Radiologic accidents or terrorist acts involving radioactive material, as well as radiation exposure in medical or industrial procedures are potential sources of risk for human health. All these risks share a common element, exposure to ionizing radiation. The extent of ionizing radiation injury will depend on a numbe...
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pub_type: 杂志文章,评审
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更新日期:2019-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2021-01-19 00:00:00
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journal_title:International journal of radiation biology
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更新日期:1995-10-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:2011-01-01 00:00:00
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更新日期:2016-01-01 00:00:00
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更新日期:2010-02-01 00:00:00
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更新日期:2010-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-04-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:2009-08-01 00:00:00
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更新日期:1997-05-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:2010-01-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:1995-01-01 00:00:00
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更新日期:2015-02-01 00:00:00