Abstract:
:We have measured clonal survival following gamma-irradiation of unstimulated (G0) T-lymphocytes from 35 donors, of 11 T-lymphocyte cell lines, of six lymphoblastoid cell lines, and of nine primary fibroblast strains for which we have G0 T-lymphocyte material from the same donor. Amongst the G0 lymphocytes we have results from nine normal donors, from eight cord bloods, from seven ataxia-telangiectasia (A-T) patients and from nine A-T heterozygotes. Although there is some variation between samples, G0 T-lymphocytes from normal donors appear to be slightly more radioresistant than T-lymphocyte lines, with a more shouldered survival curve. From our limited sample, lymphoblastoid cell lines appear to be slightly more radiosensitive than T-lymphocytes. The overall radiosensitivity of primary fibroblasts appears to be broadly similar to that of G0 T-lymphocytes. In nine instances, five A-Ts and four A-T heterozygotes, both G0 T-lymphocytes and primary fibroblasts from the same donor were tested. In five cases there was closely similar radiosensitivity in the two cell types, but in four cases there was some discrepancy. Further work, especially with normal donors, will be required in order to establish how reliably radiosensitivity in other cell types can be predicted from that of G0 T-lymphocytes. In all cell types the hypersensitivity of A-T cells was confirmed. Furthermore, the marginally greater sensitivity of A-T heterozygotes, when compared as a group with normals, was confirmed with G0 T-lymphocytes. Our results also suggest a slightly increased radiosensitivity in G0 T-lymphocytes from some, but not all, cord blood samples.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Cole J,Arlett CF,Green MH,Harcourt SA,Priestley A,Henderson L,Cole H,James SE,Richmond Fdoi
10.1080/09553008814552331subject
Has Abstractpub_date
1988-12-01 00:00:00pages
929-43issue
6eissn
0955-3002issn
1362-3095pii
WR80JEU7BG5MG6XGjournal_volume
54pub_type
杂志文章abstract:PURPOSE:Multicellular tumour spheroids are being used with increasing frequency in various aspects of tumour biology, including studies dealing with radiation biology. This review attempts to outline recent studies using these three-dimensional systems in radiation biology with particular reference made to papers testi...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
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更新日期:2020-12-03 00:00:00
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journal_title:International journal of radiation biology
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更新日期:2008-08-01 00:00:00
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pub_type:
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更新日期:1998-05-01 00:00:00
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更新日期:2008-12-01 00:00:00
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更新日期:1990-12-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:2006-02-01 00:00:00
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更新日期:2021-01-19 00:00:00
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journal_title:International journal of radiation biology
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更新日期:1994-11-01 00:00:00
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更新日期:2001-10-01 00:00:00
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更新日期:1996-09-01 00:00:00
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