The yield of DNA double-strand breaks produced intracellularly by ionizing radiation: a review.


:The mechanisms of radiation damage production are used to examine the following premises: (1) the number of DNA double-strand breaks per unit dose increases with dose; (2) cell type to cell type variations in yield of DNA dsb per dose occur. Two stages of damage production are identified as possible sources of damage yield modulation; numbers of OH. free radicals reacting with the target, and amount of chemical repair occurring on the target radicals. These factors are discussed in the light of the structures within which cellular DNA is packaged and the known rate constants for the reactions involved. It is concluded from our current knowledge that, in the presence of oxygen: (a) the number of DNA dsb is linearly related to dose, and (b) the yields of DNA damage per dose among cell types are constant. There is a caveat to the latter conclusion: the chromatin structure may be different in radiosensitive cell lines. In the absence of such a difference, variations in radiosensitivity with dose or with cell type are assigned to differences in repair speed and/or accuracy.


Int J Radiat Biol


Ward JF




Has Abstract


1990-06-01 00:00:00














  • Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry.

    abstract:PURPOSE:The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of thi...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Grégoire E,Roy L,Buard V,Delbos M,Durand V,Martin-Bodiot C,Voisin P,Sorokine-Durm I,Vaurijoux A,Voisin P,Baldeyron C,Barquinero JF

    更新日期:2018-03-01 00:00:00

  • Detection and sequencing of ionizing radiation-induced DNA rearrangements using the inverse polymerase chain reaction.

    abstract:PURPOSE:To develop a procedure, using the inverse polymerase chain reaction, to detect and sequence ionizing radiation-induced DNA rearrangements without prior phenotypic selection of mutant cells. METHOD:Normal human fibroblast cells (IMR-90) were given 30Gy of gamma-irradiation and then incubated at 37 degrees C for...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Forrester HB,Radford IR

    更新日期:1998-07-01 00:00:00

  • Effects of 50 Hz extremely low frequency sinusoidal magnetic fields on the apoptosis of the hearts of preincubated chicken embryos at different levels of developments.

    abstract:PURPOSE:The aim of this research was to demonstrate the effects of 50 Hz sinusoidal extremely low frequency of magnetic fields (ELF-MF) on preincubated chicken embryo hearts at histopathological, biochemical, and ultrastructural levels. MATERIALS AND METHODS:Ninety healthy fertilized eggs were divided into six groups ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Shams Lahijani M,Tehrani DM,Fereydouni N

    更新日期:2013-04-01 00:00:00

  • Genetic changes in progeny of bystander human fibroblasts after microbeam irradiation with X-rays, protons or carbon ions: the relevance to cancer risk.

    abstract:PURPOSE:Radiation-induced bystander effects have important implications in radiotherapy. Their persistence in normal cells may contribute to risk of health hazards, including cancer. This study investigates the role of radiation quality and gap junction intercellular communication (GJIC) in the propagation of harmful e...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Autsavapromporn N,Plante I,Liu C,Konishi T,Usami N,Funayama T,Azzam EI,Murakami T,Suzuki M

    更新日期:2015-01-01 00:00:00

  • Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans - joint RENEB and EURADOS inter-laboratory comparisons.

    abstract:PURPOSE:RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ainsbury E,Badie C,Barnard S,Manning G,Moquet J,Abend M,Antunes AC,Barrios L,Bassinet C,Beinke C,Bortolin E,Bossin L,Bricknell C,Brzoska K,Buraczewska I,Castaño CH,Čemusová Z,Christiansson M,Cordero SM,Cosler G,Mo

    更新日期:2017-01-01 00:00:00

  • Chronic exposure to bryostatin-1 increases the radiosensitivity of U937 leukaemia cells ectopically expressing Bcl-2 through a non-apoptotic mechanism.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cartee L,Davis C,Lin PS,Grant S

    更新日期:2000-10-01 00:00:00

  • Reaction of guanyl radicals in plasmid DNA with biological reductants: chemical repair of DNA damage produced by the direct effect of ionizing radiation.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Milligan JR,Aguilera JA,Mares EJ,Paglinawan RA,Ward JF

    更新日期:2001-11-01 00:00:00

  • Ultra-low dose beta-irradiation induces constriction of rabbit carotid arteries via the endothelium.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Zhirnov VV,Iakovenko IN

    更新日期:2005-11-01 00:00:00

  • Late effects of radiation on the human immune system: an overview of immune response among the atomic-bomb survivors.

    abstract::The studies of the late effects of atomic-bomb (A-bomb) radiation on the immune system were started about 20 years after the bombings in 1945. The most remarkable late effects of radiation are the functional and quantitative abnormalities of T and B cells in survivors exposed to high doses (> or = 1.0 Gy). Abnormaliti...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Akiyama M

    更新日期:1995-11-01 00:00:00

  • Hypersensitivity of a human tumour cell line to very low radiation doses.

    abstract::Survival of HT29 cells was measured after irradiation with single doses of X-rays (0.05-5 Gy) and neutrons (0.025-1.5 Gy), using a Dynamic Microscopic Imaging Processing Scanner (DMIPS) with which individual cells can be accurately located in tissue culture flasks, their positions recorded, and after an appropriate in...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Lambin P,Marples B,Fertil B,Malaise EP,Joiner MC

    更新日期:1993-05-01 00:00:00

  • Interaction between gene repair and mobile elements-induced activity systems after low-dose irradiation.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Yushkova E,Zainullin V

    更新日期:2016-09-01 00:00:00

  • The effects of gamma rays on the regeneration of hair follicles are carried over to later hair cycles.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Sugaya K,Ishihara Y,Inoue S,Hirobe T

    更新日期:2015-01-01 00:00:00

  • Experimental anti-tumor therapy in 3-D: spheroids--old hat or new challenge?

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Friedrich J,Ebner R,Kunz-Schughart LA

    更新日期:2007-11-01 00:00:00

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    更新日期:2014-06-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 临床试验,杂志文章


    authors: Hao Y,Keane T

    更新日期:1994-06-01 00:00:00

  • The shape of DNA elution dose-response curves under non-denaturing conditions: the contribution of the degree of chromatin condensation.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Okayasu R,Iliakis G

    更新日期:1992-04-01 00:00:00

  • DNA strand-break yields after post-irradiation incubation with base excision repair endonucleases implicate hydroxyl radical pairs in double-strand break formation.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Milligan JR,Aguilera JA,Nguyen TT,Paglinawan RA,Ward JF

    更新日期:2000-11-01 00:00:00

  • Comet Assay analysis of DNA strand breaks after exposure to the DNA-incorporated Auger Electron Emitter Iodine-125.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Unverricht-Yeboah M,Holtmann K,Kriehuber R

    更新日期:2020-12-03 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Paul CR,Budzinski EE,Maccubbin A,Wallace JC,Box HC

    更新日期:1990-11-01 00:00:00

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    pub_type: 杂志文章


    authors: van den Aardweg GJ,Hopewell JW,Adams GE,Barnes DW,Sansom JM,Stratford IJ,Nethersell AB

    更新日期:1991-04-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Laszlo A,Davidson T,Hu A,Landry J,Bedford J

    更新日期:1993-05-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Prabhakar KR,Veerapur VP,Parihar KV,Priyadarsini KI,Rao BS,Unnikrishnan MK

    更新日期:2006-08-01 00:00:00

  • Desferrithiocin analogue uranium decorporation agents.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Bergeron RJ,Wiegand J,Singh S

    更新日期:2009-04-01 00:00:00

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    abstract::Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Wallace SS

    更新日期:1994-11-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Mateos S,Gordon AT,Steel GG,McMillan TJ

    更新日期:1996-06-01 00:00:00

  • Chromosome painting of radiation-induced micronuclei.

    abstract::In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Slavotinek A,Sauer-Nehls S,Braselmann H,Taylor GM,Nüsse M

    更新日期:1996-10-01 00:00:00

  • Similarities in the repair kinetics of sublethal and potentially lethal X-ray damage in log phase Chinese hamster V79 cells.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Reddy NM,Lange CS

    更新日期:1989-09-01 00:00:00

  • Radioprotection of C3H mice by recombinant human interleukin-1 alpha.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Wu SG,Tuboi A,Miyamoto T

    更新日期:1989-10-01 00:00:00

  • Does incomplete repair explain the apparent failure of the basic LQ model to predict spinal cord and kidney responses to low doses per fraction?

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Thames HD,Ang KK,Stewart FA,van der Schueren E

    更新日期:1988-07-01 00:00:00

  • Topoisomerase activities and levels in irradiated Chinese hamster AA8 cells and in its radiosensitive mutant EM9.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Pastor N,Piñero J,Ortiz T,Mateos JC,De Miguel M,Cortés F

    更新日期:1999-08-01 00:00:00