Abstract:
:We studied the extent of split-dose recovery in seven non-transformed human fibroblast cell lines of different intrinsic radiosensitivity (HF19, 1BR3, 149BR, 84BR, GM739, 180BR and AT2EM). Experiments were performed on both growing and plateau-phase cells. The seven cell lines displayed a wide range of intrinsic radiosensitivity. The D of plateau phase cells ranged from 0.56 (AT2EM) to 3.02 Gy (HF19). The recovery ratios (RR) of the three non-ataxic hypersensitive cell lines (84BR, GM739, and 180BR) were significantly higher than those predicted from the single-dose survival curves of both growing and plateau-phase cells. In addition, in these three hypersensitive cell lines the challenge dose survival curve generated after different priming doses showed a reduction in the intrinsic radiosensitivity; the high RRs observed were due both to beta and a reduction in alpha. This suggests that a protective mechanism may be triggered by the first irradiation leading to induced radioresistance. For growing cells, the relationship between ln RR and 2D2 was well fitted by linear regression. With plateau phase cells, RR appeared to be dose dependent in a more complex fashion. Thus, no single value of beta RR was representative of the split-dose recovery. With the ataxic cell line AT2EM, the split-dose studies detected a limited capacity to recover in spite of the beta value of the single dose survival curve being nil.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Alsbeih MG,Fertil B,Arlett CF,Malaise EPdoi
10.1080/095530096146075subject
Has Abstractpub_date
1996-02-01 00:00:00pages
225-39issue
2eissn
0955-3002issn
1362-3095journal_volume
69pub_type
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