Abstract:
:DNA neutral (pH 9.6) filter elution was used to measure radiation-induced DNA double-strand break (dsb) frequencies in eight human squamous cell carcinoma cell lines with radiosensitivities (D0) ranging from 1.07 to 2.66 Gy and D values ranging from 1.46 to 4.08 Gy. The elution profiles of unirradiated samples from more radiosensitive cell lines were all steeper in slope than the profiles from resistant cells. The shapes of the dsb induction curves were curvilinear and there was some variability from cell line to cell line in the dose-response for the induction of DNA dsb after exposures to 5-100 Gy 60Co gamma-rays. There was no relation between the shapes of the survival curves and the shapes of the dose-responses for the induction of DNA dsb. At low doses (5-25 Gy), three out of four of the more sensitive cell lines (D less than 2.5 Gy) had larger initial break frequencies than the more resistant lines (D greater than 3.0 Gy). Although the low-dose (5-25 Gy) elution results were variable, they do suggest that DNA neutral elution will detect differences between sensitive and resistant tumour cells in initial DNA dsb frequencies.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Schwartz JL,Mustafi R,Beckett MA,Czyzewski EA,Farhangi E,Grdina DJ,Rotmensch J,Weichselbaum RRdoi
10.1080/09553009114551211subject
Has Abstractpub_date
1991-06-01 00:00:00pages
1341-52issue
6eissn
0955-3002issn
1362-3095pii
FQYTRH6U5D0376Q8journal_volume
59pub_type
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journal_title:International journal of radiation biology
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