Abstract：
:Mutations in the p53 tumour suppressor gene are found at high frequency in bladder cancer. There is strong evidence that p53 plays an important role in controlling the cell cycle after DNA damage by ionizing radiation. However, the effect of loss of p53 function on radiosensitivity is not yet clear. Radiotherapy combined with chemotherapy is the most common treatment for patients with invasive bladder cancer. Recently three bladder cancer cell lines have been established and this paper investigates the p53 status and clonogenic survival of these cell lines following irradiation. It was found that one line expresses wt p53 (UCRU-BL-13) and two lines contain a codon 72 polymorphism (UCRU-BL-17 and UCRU-BL-28). UCRU-BL-17 cells also contain a point mutation affecting codon 280. The level of p53 expression in the cell lines is clearly different, with UCRU-BL-17 expressing a higher level of p53 compared with UCRU-BL-13; UCRU-BL-28 expressed intermediate levels. The clonogenic survival of these cell lines has been determined. It was found that the line expressing a p53 mutation was more sensitive than those with wild type p53, providing support for a model in which loss of p53 function is associated with increased radiosensitivity, possibly due to reduced p53-dependent DNA repair.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Ribeiro JC,Barnetson AR,Fisher RJ,Mameghan H,Russell PJdoi
10.1080/095530097143491subject
Has Abstractpub_date
1997-07-01 00:00:00pages
11-20issue
1eissn
0955-3002issn
1362-3095journal_volume
72pub_type
杂志文章abstract：PURPOSE:To investigate alternative scenarios for the dose-dependent emission of bystander signals by irradiated cells in medium transfer experiments. METHODS:Energy deposition patterns to hypothetical intracellular targets whose hit by radiation initiates the emission of bystander signals have been simulated by Monte ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.595874
更新日期：2012-01-01 00:00:00
abstract：:Primary chick embryo myoblasts can be a useful tool for studying the developmental events which accompany myoblast differentiation, particularly myoblast membrane fusion. To determine whether the electrical properties and/or fusion in these systems are affected by 50 Hz magnetic fields, chick embryo myoblast cultures ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:We used three common fluorescent probes to measure pericentric inversion frequencies in 2.9 Gy 60Co gamma-irradiated human lymphocytes. For a given chromosome, the first probe is specific to one telomeric region, the second probe is specific to one subcentromeric region and the third probe is specific to the centromer...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144382
更新日期：1997-01-01 00:00:00
abstract：PURPOSE:The relationship between cellular telephone use and excretion of the melatonin metabolite 6-hydroxymelatonin sulfate (6-OHMS) was evaluated in two populations of male electric utility workers (Study 1, n=149; Study 2, n=77). MATERIALS AND METHODS:Participants collected urine samples and recorded cellular telep...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000210166561
更新日期：2002-11-01 00:00:00
abstract：PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.809170
更新日期：2013-11-01 00:00:00
abstract：PURPOSE:To investigate possible variations in topoisomerase (topo) I and II activities and levels after X-ray treatment in the radiation repair proficient AA8 Chinese hamster cell line for comparison with the radiation sensitive mutant EM9. MATERIALS AND METHODS:AA8 and EM9 cells were irradiated with 5 Gy of X-rays an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139809
更新日期：1999-08-01 00:00:00
abstract：PURPOSE:Multicellular tumour spheroids are being used with increasing frequency in various aspects of tumour biology, including studies dealing with radiation biology. This review attempts to outline recent studies using these three-dimensional systems in radiation biology with particular reference made to papers testi...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/095530099139845
更新日期：1999-07-01 00:00:00
abstract：PURPOSE:This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after γ-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. METHODS:MSC were irradiated with 2 and 20 Gy by (60)Co. For ATM inhibition, specific inhibitor KU5593...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.775530
更新日期：2013-07-01 00:00:00
abstract：:The structure of mouse skin collagen fibrils after treatment with 0.5, 1 and 2.5 Gy gamma-irradiation was studied by electron microscopy. Animals were sacrificed 1, 4 and 8 weeks after irradiation. Although there were areas where the normal parallel packing of fibrils was retained in some regions packing was interrupt...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144481
更新日期：1997-01-01 00:00:00
abstract：:The rate of recovery from radiation damage, as a function of dose per fraction, was investigated in mouse skin. Two different experimental designs were used, both incorporating the neutron top-up technique which enables the X-ray dose per fraction to be kept constant whilst changing the interfraction interval. Either ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550461
更新日期：1991-02-01 00:00:00
abstract：:Objective: To quantify DNA damage in patients undergoing non-contrast and contrast-enhanced 18F-FDG PET/CT whole body positron emission tomography/computed tomography (WB PET/CT) investigations using comet assay technique and micronucleus assay, and to study the effect of other baseline parameters of patients on DNA d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1577569
更新日期：2019-06-01 00:00:00
abstract：:The aim was to review and summarize the results of 40 years of study concerning the response to ionizing radiation of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in sensitivity to various DNA-damaging agents, among them X- and gamma-rays. The reviewed data indicate the key importance of DNA damage rep...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000500143534
更新日期：2005-05-01 00:00:00
abstract：:CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551361
更新日期：1993-08-01 00:00:00
abstract：PURPOSE:Human embryonic stem cells (hESC) hold a great potential for regenerative medicine because, in principle, they can differentiate into any cell type found in the human body. In addition, studying the effect of ionizing radiation (IR) on hESC may provide valuable information about the response of human cells to I...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.700435
更新日期：2012-12-01 00:00:00
abstract：:The effect of a siderophore analogue 3,4,3-LIHOPO has been investigated in rat after intramuscular injection of 238Pu, 239Pu and 241Am simulating puncture wounds. Various treatment regimens were used to remove the radioactivity from its injection site and to reduce its retention in body tissues. The local deposits cou...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145382
更新日期：1996-07-01 00:00:00
abstract：PURPOSE:Since the exact development of posterior subcapsular cataracts (PSCs) is poorly understood, we review various risk factors and propose a two-stage etiology for PSCs. METHODS:The biological mechanisms associated with age-related cataracts (primarily nuclear cataracts, cortical cataracts and PSCs) were reviewed ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1812759
更新日期：2020-11-01 00:00:00
abstract：PURPOSE:Genotoxic effects have been considered the gold standard to determine if an environmental factor is a carcinogen, but the currently available data for extremely low frequency time-varying magnetic fields (ELF-MF) remain controversial. As an environmental stimulus, the effect of ELF-MF on cellular DNA may be sub...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.887866
更新日期：2014-04-01 00:00:00
abstract：PURPOSE:In order to understand the molecular mechanism of nucleobase damage caused by ultrasoft X-ray irradiation, guanine radicals have been studied using an X-band EPR (electron paramagnetic resonance) spectrometer installed in a synchrotron soft X-ray beamline. MATERIALS AND METHODS:Guanine pellets were irradiated ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000400017689
更新日期：2004-11-01 00:00:00
abstract：PURPOSE:To study the influence of TGF-beta1 and TGF-beta-neutralizing antibodies on the clonogenic activity and terminal differentiation of rat lung fibroblasts following radiation exposure. MATERIAL AND METHODS:Early passage rat lung fibroblasts were used in this study. Colony formation assays were applied to determi...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138501
更新日期：2000-04-01 00:00:00
abstract：:The role of protein kinase C in radiation-induced death of thymocytes was studied. For this purpose murine thymocytes were irradiated and incubated for 6 h at 37 degrees C and afterwards the fraction of fragmented DNA was measured. Results indicate that radiation-induced DNA fragmentation can be prevented by adding th...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551471
更新日期：1992-05-01 00:00:00
abstract：PURPOSE:To reduce retention and toxicity of the alpha particle emitter polonium-210 in rats by newly developed chelating agents. MATERIALS AND METHODS:Repeated subcutaneous chelation was conducted after intravenous injection of 210Po nitrate. For reduction of 210Po retention the treatment with vicinal dithiols meso-an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050151691
更新日期：2000-10-01 00:00:00
abstract：:The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551951
更新日期：1990-10-01 00:00:00
abstract：:BW12C, which was developed as an agent for the treatment of sickle cell anaemia, increases the binding of oxygen to haemoglobin and hence reduces the availability of oxygen to tissues. Due to these changes in oxygen availability BW12C could act as a protector against radiation-induced injury to normal tissues. In this...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114550921
更新日期：1991-04-01 00:00:00
abstract：PURPOSE:To evaluate the cumulative gamma-radiation personal exposure by analysing lymphocyte chromosome translocations using FISH painting and to compare FISH-derived biodoses with those derived from retrospective physical dose reconstruction in residents receiving chronic low dose-rate gamma-irradiation while living i...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110053873
更新日期：2001-07-01 00:00:00
abstract：:The in vitro cell survival of a human cervix carcinoma cell line (HX156c) has been assessed using 60Co gamma-rays administered at either high (150 cGy/min) or low (3.2 cGy/min) dose rate. Recovery during low dose-rate irradiation was observed; the dose reduction factor at 10(-2) cell kill for 150 versus 3.2 cGy/min wa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814551671
更新日期：1988-08-01 00:00:00
abstract：PURPOSE:This review will examine the role of phosphatases in cancer cell signalling and also outline emerging findings regarding the influence of phosphatases on tumor cell survival after ionising radiation. CONCLUSION:The exposure of tumour cells to clinically relevant doses of ionising radiation causes DNA damage an...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553000903232827
更新日期：2009-11-01 00:00:00
abstract：:Induction by gamma-radiation, UV radiation or hydroxyurea of RecA gene product synthesis in Escherichia coli, monitored as beta-D-galactosidase in recA-lacZ fusion strains, was shown to be inhibited if 2-mercaptoethylamine (MEA) was added before treatment with the inducing agents. If cysteine (Cys) at low concentratio...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096146228
更新日期：1996-01-01 00:00:00
abstract：PURPOSE:The identification of fibroblast-like cells of the marrow stroma by means of alkaline phosphatase (ALP) cytochemistry reveals delicate ALP-positive structures interspersed among haematopoietic cells and arranged in a loosely meshed network. These cells are often referred to as 'reticular' cells and the network ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/0955300031000085740
更新日期：2003-04-01 00:00:00
abstract：PURPOSE:The deleterious processes triggered by heavy ions on DNA were studied through the determination of the yield of a series of oxidized bases. Emphasis was placed on the estimation of the respective contribution of direct ionization and indirect effects, mostly by comparison with low linear energy transfer (LET) g...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000600573788
更新日期：2006-02-01 00:00:00
abstract：PURPOSE:To calculate survival of human haemopoietic stem cells irradiated by alpha particles from 149Tb (initial energy 3.97 MeV) and 211At (average initial energy 6.87 MeV). METHODS:Following as closely as possible radiobiological data, Monte Carlo methods were used to calculate passages of alpha particles (originati...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141771
更新日期：1998-07-01 00:00:00
