Abstract:
:Solid human tumours contain areas with low oxygen tension (pO2). For bioreductive drugs it is important to define the cytotoxic effect according to drug concentration and to clinically relevant pO2. In this study, the pO2 dependence of the survival of three human cell lines (HRT 18, Na11 +, and MEWO), exposed to tirapazamine (SR-4233) alone or combined with ionizing radiation, was studied in vitro. Gas changes were made to obtain five different oxygen concentrations: air (20.9% O2), 10, 2, 0.2 and 0.02% O2 (hypoxia). Tirapazamine below a concentration of 100 microM was not cytotoxic in air or at 10% O2. At 100 microM tirapazamine was toxic in 2% O2, and at 50 microM in 0.2% O2. For pO2 < 0.2% O2, there was a marked increase in cell killing when 10 microM tirapazamine was combined with 2 Gy, compared with either 10 microM or 2 Gy given alone (p < 0.03). The cytotoxic effect of tirapazamine on human tumour cells in vitro is highly dependent on clinically relevant pO2's. The activation of tirapazamine at a low concentration and at a pO2 found mainly in tumours could yield a very beneficial therapeutic ratio.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Lartigau E,Guichard Mdoi
10.1080/09553009514550261subject
Has Abstractpub_date
1995-02-01 00:00:00pages
211-6issue
2eissn
0955-3002issn
1362-3095pii
0H9LN46QR2N8Q309journal_volume
67pub_type
杂志文章abstract:PURPOSE:To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. MATERIALS AND METHODS:Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20 h ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139700
更新日期:1999-08-01 00:00:00
abstract::Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097144265
更新日期:1997-02-01 00:00:00
abstract:PURPOSE:Human embryonic stem cells (hESC) hold a great potential for regenerative medicine because, in principle, they can differentiate into any cell type found in the human body. In addition, studying the effect of ionizing radiation (IR) on hESC may provide valuable information about the response of human cells to I...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.700435
更新日期:2012-12-01 00:00:00
abstract::Transforming growth factor-beta (TGF beta 1) plays a central role in wound healing, so its perturbation by radiation may contribute to the acute and late effects seen in irradiated skin. TGF beta 1 mRNA expression was measured by PCR, in the skin of the CD1 and CBA mouse, exposed to Sr-90 beta from an 11-mm diameter s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551231
更新日期:1995-09-01 00:00:00
abstract:PURPOSE:Ionizing radiation has been recognized to increase the risk of cardiovascular diseases (CVD). However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. MATERIAL AND METHODS:Previously, human umbilical vein endothe...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.905724
更新日期:2014-07-01 00:00:00
abstract:PURPOSE:To study the oxidative stress-induced changes by sublethal doses of cadmium chloride (CdCl2) and gamma irradiation, two redox-inducing agents, on metallothionein (MT) gene and protein expression in Plantago ovata Forsk (P. ovata). MATERIALS AND METHODS:Chlorophyll content was estimated to study the stress resp...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.782109
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:To find detectable cytogenetic biomarkers that can offer information about the radiation quality of in vivo exposure retrospectively. MATERIALS AND METHODS:Chromosome-type aberrations of peripheral lymphocytes of uterine cancer patients that received internal gamma- and external X-ray therapy or carbon beam th...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000600863064
更新日期:2006-07-01 00:00:00
abstract::Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145634
更新日期:1996-05-01 00:00:00
abstract:PURPOSE:To investigate the p53 ionizing radiation-induced response, G1/S cell-cycle block and cytogenetic damage in Nijmegen breakage syndrome (NBS) cells characterized by different haplotypes. MATERIAL AND METHODS:Lymphoblastoid cell lines derived from three normals, five NBS and two ataxia telangiectasia (AT) indivi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140221
更新日期:1999-05-01 00:00:00
abstract::Recent evidence indicates that isoeffect doses for spinal cord and kidney may be overestimated for fraction sizes as small as 1 or 2 Gy when calculated from a linear-quadratic (LQ) model fitted to data obtained for fraction sizes larger than 2 Gy. Reasons for this are unknown, but possible interpretations include exha...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551461
更新日期:1988-07-01 00:00:00
abstract:PURPOSE:To study the absorptive function of rat colon following whole-body exposure to neutron irradiation, either to the same total dose with varying proportion of neutrons or to the same neutron proportion with an increasing irradiation dose. MATERIALS AND METHODS:Different proportions of neutron irradiation were pr...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300021000019221
更新日期:2002-12-01 00:00:00
abstract::Purpose: Ionizing radiation is a risk factor to the whole organism, including the heart. Cardiac damage is considered to be a late effect of radiation exposure. While the acute cardiotoxicity of high doses is well characterized, the knowledge about nature and magnitude of the cardiac risk following lower doses exposur...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2020.1704299
更新日期:2020-03-01 00:00:00
abstract::Because of the role that alveolar macrophages (PAM) play in the pulmonary clearance of inhaled particles via mechanical transport and dissolution, understanding the uptake and dissolution of particles by these cells might provide insight into the mechanisms of particle dissolution in lungs of various species and hence...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551861
更新日期:1992-07-01 00:00:00
abstract::In a series of studies to investigate activation energies and thermal enhancements of various chemotherapeutic agents the effect of 5-fluoruracil (5FU), an antimetabolite, on murine tumour cells was studied at elevated temperatures. Animal tumours were early generation isotransplants of a spontaneous fibrosarcoma, FSa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550211
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. MATERIALS AND METHODS:Macrocolony formation wa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.894650
更新日期:2014-05-01 00:00:00
abstract::High doses of rHIL-1 protected C3H/HeN mice against lethal radiation damage. The optimum time of radioprotection was found when this cytokine was administered at 4 h and at 20 h before irradiation. The dose reduction factors when the IL-1 was administered at those times were about 1.30. The radioprotection at the opti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551621
更新日期:1989-10-01 00:00:00
abstract::Intraoperative irradiation (IORT) is used to enhance local tumour control by using large, single doses while removing critical structures from the treatment field. Peripheral nerve remains a dose-limiting normal tissue that often cannot be removed from the field. To assess ultrastructural changes in canine sciatic ner...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550941
更新日期:1995-07-01 00:00:00
abstract:PURPOSE:To test the importance of serotonin as a signaling molecule involved in the production and response of radiation-induced bystander effects. MATERIALS AND METHODS:HPV-G human keratinocyte cultures were spiked with various concentrations of Granisetron or Ketanserin and subject to either 0 Gy or 0.5 Gy X-irradia...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.714519
更新日期:2012-10-01 00:00:00
abstract::Induction by gamma-radiation, UV radiation or hydroxyurea of RecA gene product synthesis in Escherichia coli, monitored as beta-D-galactosidase in recA-lacZ fusion strains, was shown to be inhibited if 2-mercaptoethylamine (MEA) was added before treatment with the inducing agents. If cysteine (Cys) at low concentratio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146228
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:To present details of the recent version of the 'Local Effect Model' (LEM), that has been developed and implemented in treatment planning for the ion beam therapy pilot project performed at GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt, Germany. MATERIALS AND METHODS:The new version of the model i...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.611213
更新日期:2012-01-01 00:00:00
abstract::Resolving the molecular mechanisms of radiation oncogenesis represents an important but daunting challenge in radiation research. This brief review outlines the principal oncogenic mechanisms that need to be considered in the context of radiation effects on the genome, how these might relate to specific gene and chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414550081
更新日期:1994-01-01 00:00:00
abstract:PURPOSE:To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. MATERIALS AND METHODS:Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.556175
更新日期:2011-05-01 00:00:00
abstract::The relationship between cellular radiosensitivity and normal-tissue response to radiotherapy in individual cancer patients has attracted increasing attention over the last few years. Recent work has suggested that a correlation exists between fibroblast sensitivity and normal-tissue reactions. We have examined the ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145021
更新日期:1996-09-01 00:00:00
abstract::Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552451
更新日期:1992-11-01 00:00:00
abstract:PURPOSE:To examine the heat sensitivity of DNA-dependent protein kinase (DNA-PK) activity in a variety of cultured mouse, hamster and human cell lines. MATERIALS AND METHODS:Eight cell lines, which have been routinely used in our laboratory, were examined. Cells were heated at 44.0 +/- 0.05 degrees C and DNA-PK activi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001596959
更新日期:2003-08-01 00:00:00
abstract::The relative contributions of the OH-mediated and direct radiation effect on the induction of DNA double-strand breaks (dsbs) were evaluated in two haploid yeast cell lines (GSH+ and gsh-) irradiated under oxic or hypoxic conditions in the absence or presence of 6 M glycerol as an OH radical scavenger. Gsh- cells are ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551721
更新日期:1993-11-01 00:00:00
abstract::Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1571256
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To evaluate how irradiation affects thyroid autoimmunity in mouse models of Hashimoto's thyroiditis and Graves' hyperthyroidism. MATERIALS AND METHODS:Non-obese diabetic (NOD)-H2(h4) mice spontaneously develop anti-thyroglobulin (Tg) antibodies and thyroiditis when supplied with sodium iodine (NaI) in the drin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802345910
更新日期:2008-09-01 00:00:00
abstract:PURPOSE:This review is a contribution to the memory of Dr William (Bill) Morgan and highlights an area of research and deliberation that he considered extremely important in support of the setting of protective radiation dose limits. Biological research has generally played a minor role in the estimation of adverse hea...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1290848
更新日期:2017-10-01 00:00:00
abstract:PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802499212
更新日期:2008-11-01 00:00:00