Abstract:
PURPOSE:To investigate the effect of gamma radiation-induced alterations in antioxidant defence of radioresistant Sf9 insect cells. MATERIALS AND METHODS:Sf9 cells were irradiated at doses ranging from 0.5-200 Gy. Lipid peroxidation and protein carbonylation levels were observed at 4 h post-exposure along with reduced glutathione/oxidized glutathione (GSH/GSSG) profile as well as specific activities of redox active enzymes superoxide dismutase (SOD), catalase, ascorbate peroxidase (APOx), and glutathione reductase (GR). Human brain malignant glioma (BMG-1) cells were used for comparing radiation response of mammalian cells. RESULTS:Sf9 cells displayed significantly less radiation-induced reactive oxygen/nitrogen species (ROS/RNS) generation, protein carbonylation and growth inhibition as compared to mammalian cells. Sf9 cells have higher basal APOx (∼4-fold), catalase (∼1.7-fold), SOD (∼1.3-fold) activity and GSH level (∼2.2-fold) compared to mammalian cells. A radiation dose-dependent increase in SOD, Catalase and APOx activity was found in Sf9 cells at least up to 100 Gy dose, while maximum activity in mammalian cells was achieved by 10 Gy. CONCLUSION:The present study suggests that Lepidopteran insect cells carry a stronger antioxidant system that protects against radiation-induced macromolecular damage, growth inhibition and cell death.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Suman S,Khan Z,Zarin M,Chandna S,Seth RKdoi
10.3109/09553002.2015.1054958subject
Has Abstractpub_date
2015-01-01 00:00:00pages
732-41issue
9eissn
0955-3002issn
1362-3095journal_volume
91pub_type
杂志文章abstract::The past recommendations of the ICRP about dose limits to the skin are reviewed. Recently, an ICRP Task Group has been revisiting the old arguments and setting them against new data. With the exception of the function of cells in the skin associated with immunocompetence, non-stochastic effects have been well characte...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014550961
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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更新日期:2017-11-01 00:00:00