Abstract：
PURPOSE:In case of a large-scale radiation accident when hundreds of people may be exposed, it is important to distinguish the severely exposed individuals (> or =1 gray), who require early medical treatment, from those less exposed. The aim of our study was to develop a quick population triage method based on automated micronucleus (MN) scoring. MATERIALS AND METHODS:Using the MN software module developed by MetaSystems specifically for the Metafer4 platform, about 60 blood samples can be scored in one day. Standard dose response curves were determined for manual and automated MN scoring. RESULTS:The automated MN assay results were closely correlated with MN yields obtained with the manual procedure. A dose of 1 Gy can be estimated with an uncertainty of 0.2 Gy. Corrections for false positives and false negatives by visual inspection of the image gallery did not result in an improved accuracy or reproducibility. To test the automated MN assay in a multicenter setting, an inter-laboratory comparison was performed whereby irradiated blood samples were processed in Ghent University (Belgium) and BfS (Bundesamt fuer Strahlenschutz; Germany). Both laboratories obtained comparable results. CONCLUSIONS:These results confirm the efficacy of the automated MN assay for fast population triage in a multicenter setting, in the case of large radiation accidents.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Willems P,August L,Slabbert J,Romm H,Oestreicher U,Thierens H,Vral Adoi
10.3109/09553000903264481subject
Has Abstractpub_date
2010-01-01 00:00:00pages
2-11issue
1eissn
0955-3002issn
1362-3095journal_volume
86pub_type
杂志文章abstract：PURPOSE:To calculate the depth distribution of radioactive decays in bone by fitting a calculated alpha-particle energy spectrum to a measured spectrum. The dose to bone surface cells is then calculated from the depth distribution. MATERIALS AND METHODS:The spectra are calculated using Monte Carlo methods and a numeri...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098142734
更新日期：1998-01-01 00:00:00
abstract：PURPOSE:This review is a contribution to the memory of Dr William (Bill) Morgan and highlights an area of research and deliberation that he considered extremely important in support of the setting of protective radiation dose limits. Biological research has generally played a minor role in the estimation of adverse hea...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2017.1290848
更新日期：2017-10-01 00:00:00
abstract：:The radiation chemistry of the oligomer d(TpApCpG) X-irradiated in aqueous solution containing glutathione was studied. Four products were isolated by HPLC and characterized by NMR spectroscopy. Two of the major products are isomers of a 5-hydroxy-5,6-dihydrothymine modification of d(TpApCpG). A dihydrothymine modific...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552141
更新日期：1990-11-01 00:00:00
abstract：:The relationship between cellular radiosensitivity and normal-tissue response to radiotherapy in individual cancer patients has attracted increasing attention over the last few years. Recent work has suggested that a correlation exists between fibroblast sensitivity and normal-tissue reactions. We have examined the ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145021
更新日期：1996-09-01 00:00:00
abstract：PURPOSE:To study the natural radionuclides in the freshwater fish samples around the uranium mining areas of Bagjata and Banduhurang, East Singhbhum, Jharkhand, India. MATERIALS AND METHODS:The naturally occurring radioisotopes of uranium, U(nat), consisting of (234)U, (235)U and (238)U; (226)Ra, (230)Th and (210)Po w...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.492490
更新日期：2010-11-01 00:00:00
abstract：PURPOSE:This review will examine the role of phosphatases in cancer cell signalling and also outline emerging findings regarding the influence of phosphatases on tumor cell survival after ionising radiation. CONCLUSION:The exposure of tumour cells to clinically relevant doses of ionising radiation causes DNA damage an...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553000903232827
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:To study the effects of 12C-beam of 295 keV/microm (57.24 MeV) on M5 and Chinese hamster V79 cells by using cytogenetic assays like micronuclei (MN) induction, chromosomal aberrations (CA) and apoptosis. Additionally, the relative survival of these two cell lines was tested by the colony forming ability of the ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000601085964
更新日期：2007-01-01 00:00:00
abstract：PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802499212
更新日期：2008-11-01 00:00:00
abstract：:Photoirradiation of aqueous solutions of DNA in the presence of Al- or Zn-tetrasulphonated phthalocyanines (AIPcS4 and ZnPcS4) causes formation of strand breaks and liberation of nucleobases. The effect of added D2O, which enhances singlet oxygen (1O2) lifetime, radical scavengers including alcohols and the spin-trap ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:We have examined the timing of gamma-irradiation-induced death in relation to cell cycle progression using a panel of mouse lymphoid or myeloid cell lines. Death was found to occur immediately after irradiation ('rapid interphase' death), or after arrest in G2 phase ('delayed interphase' death). In part II of this ser...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550261
更新日期：1994-02-01 00:00:00
abstract：PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701570196
更新日期：2007-11-01 00:00:00
abstract：PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138745
更新日期：2000-03-01 00:00:00
abstract：PURPOSE:To investigate whether pre- and post-drug magnetic field (MF) exposure of 50 Hz, 0.2 mT has any significant effect on pentylenetetrazol (PTZ)-induced seizures in mice. MATERIAL AND METHODS:MF was generated by a pair of Helmholtz coils. Seizures were induced by PTZ injection intraperitoneally (i.p.) at a dose o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701206676
更新日期：2007-04-01 00:00:00
abstract：:We have previously described a cell-free assay that can be employed to study rejoining of radiation-induced DNA double-stranded breaks (dsb) in 'naked' DNA prepared from agarose-embedded cells using an extract of HeLa cells as a source of enzymes. Rejoining of dsb in this assay is absolutely dependent on cell extract ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143987
更新日期：1997-04-01 00:00:00
abstract：:Mouse C3H 10T1/2 cells are most sensitive to radiation-induced neoplastic transformation in the G2/M-phase of the cell cycle. When synchronized 10T1/2 cells were exposed to phorbol 12-myristate 13-acetate (TPA) after irradiation, transformation of cells not in the transformation-sensitive window was enhanced, but tran...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551971
更新日期：1993-12-01 00:00:00
abstract：PURPOSE:The long-term effect of low and moderate doses of ionizing radiation on the lens is still a matter of debate and needs to be evaluated in more detail. MATERIAL AND METHODS:We conducted a detailed histological analysis of eyes from B6C3F1 mice cohorts after acute gamma irradiation (60Co source; 0.063 Gy/min) at...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1876951
更新日期：2021-01-19 00:00:00
abstract：:Tikvah Alper's interest in science was broad, from scrapie to mammalian cells and cancer. Much of her own work focused on cell lethality, like that of many other radiobiologists, but this was natural because of the simplicity of the endpoint cell survival and its relevance to cancer therapy. Tikvah had broader interes...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143662
更新日期：1997-06-01 00:00:00
abstract：:The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550501
更新日期：1994-04-01 00:00:00
abstract：:DNA neutral (pH 9.6) filter elution was used to measure radiation-induced DNA double-strand break (dsb) frequencies in eight human squamous cell carcinoma cell lines with radiosensitivities (D0) ranging from 1.07 to 2.66 Gy and D values ranging from 1.46 to 4.08 Gy. The elution profiles of unirradiated samples from mo...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114551211
更新日期：1991-06-01 00:00:00
abstract：:In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In mos...
journal_title：International journal of radiation biology
pub_type：
doi：10.1080/095530097143220
更新日期：1997-10-01 00:00:00
abstract：PURPOSE:Ionizing radiation has been recognized to increase the risk of cardiovascular diseases (CVD). However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. MATERIAL AND METHODS:Previously, human umbilical vein endothe...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.905724
更新日期：2014-07-01 00:00:00
abstract：PURPOSE:To present details of the recent version of the 'Local Effect Model' (LEM), that has been developed and implemented in treatment planning for the ion beam therapy pilot project performed at GSI Helmholtzzentrum für Schwerionenforschung in Darmstadt, Germany. MATERIALS AND METHODS:The new version of the model i...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2011.611213
更新日期：2012-01-01 00:00:00
abstract：PURPOSE:Ionizing radiation causes various types of DNA damage e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted toward the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simul...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1851059
更新日期：2020-12-03 00:00:00
abstract：:Purpose: Cancer is one of the most common causes of mortality and morbidity worldwide. Vaccines have been emerged as an attractive approach for their capacity of eliciting long-term immune response targeting cancer cells. Attenuated avirulent Toxoplasma gondii stimulate immunity and activate antitumor cells thereby el...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1739772
更新日期：2020-06-01 00:00:00
abstract：PURPOSE:To test the importance of serotonin as a signaling molecule involved in the production and response of radiation-induced bystander effects. MATERIALS AND METHODS:HPV-G human keratinocyte cultures were spiked with various concentrations of Granisetron or Ketanserin and subject to either 0 Gy or 0.5 Gy X-irradia...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.714519
更新日期：2012-10-01 00:00:00
abstract：:Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550651
更新日期：1989-04-01 00:00:00
abstract：:Recent evidence indicates that isoeffect doses for spinal cord and kidney may be overestimated for fraction sizes as small as 1 or 2 Gy when calculated from a linear-quadratic (LQ) model fitted to data obtained for fraction sizes larger than 2 Gy. Reasons for this are unknown, but possible interpretations include exha...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814551461
更新日期：1988-07-01 00:00:00
abstract：:Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2016.1137105
更新日期：2016-01-01 00:00:00
abstract：PURPOSE:To seek the delayed appearance of chromosomal abnormalities in human fibroblasts exposed to the Auger electron emitter 125I. MATERIALS AND METHODS:Normal untransformed human fibroblasts, HF19, were exposed to a concentration of [I125]IUdR, which allowed the survival of 37% of clonogens. Chromosomal analysis us...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050050972
更新日期：2000-07-01 00:00:00
abstract：:The form of the hazard function for radiation-induced pneumonitis death in mice was investigated. 'Hazard' refers to the instantaneous failure rate at a specified time, conditional upon non-failure to that time. Thus, the hazard function describes the time profile for the risk of pneumonitis death among still-survivin...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214552551
更新日期：1992-11-01 00:00:00
