Characterization of radiation-induced damage in d(TpApCpG).

abstract：PURPOSE:To examine the heat sensitivity of DNA-dependent protein kinase (DNA-PK) activity in a variety of cultured mouse, hamster and human cell lines. MATERIALS AND METHODS:Eight cell lines, which have been routinely used in our laboratory, were examined. Cells were heated at 44.0 +/- 0.05 degrees C and DNA-PK activi...

journal_title：International journal of radiation biology

authors： Umeda N,Matsumoto Y,Yin HL,Tomita M,Enomoto A,Morita A,Mizukoshi T,Sakai K,Hosoi Y,Suzuki N

更新日期：2003-08-01 00:00:00

abstract：PURPOSE:To develop and validate non-fluorescent chromosome painting for bright-field microscopy using the peroxidase/diaminobenzidine (DAB) reaction. MATERIALS AND METHODS:Peripheral blood lymphocytes were taken from patients with uterine cancer who had received heavy-ion radiation therapy. Chromosome slides were trea...

journal_title：International journal of radiation biology

authors： Kanda R,Suzuki M,Minamihisamatsu M,Furukawa A,Odaka T,Hayata I

更新日期：1998-05-01 00:00:00

abstract：PURPOSE:To analyze the role of mus-genes repair in system activation P-elements in Drosophila melanogaster induced by a chronic exposure to low doses. MATERIALS AND METHODS:The materials were dysgenic individuals of Drosophila melanogaster with mutations in repair genes (mus101, mus205, mus304, mus308, mus309) and sim...

journal_title：International journal of radiation biology

authors： Yushkova E,Zainullin V

更新日期：2016-09-01 00:00:00

abstract：PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...

journal_title：International journal of radiation biology

authors： Chaudhry MA,Kreger B,Omaruddin RA

更新日期：2010-07-01 00:00:00

abstract：:In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In mos...

journal_title：International journal of radiation biology

authors： Böcker W,Bauch T,Müller WU,Streffer C

更新日期：1997-10-01 00:00:00

abstract：PURPOSE:To determine the relative toxicity of alpha- and beta-radiations under conditions of controlled temporal and spatial dose distribution. METHODS:Fused aluminosilicate particles were radiolabelled with either (45)Ca (a beta-emitter) or (242)Cm (an alpha-emitter). These were injected into CBA/Ca mice to give life...

journal_title：International journal of radiation biology

authors： Priest ND,Hoel DG,Brooks PN

更新日期：2010-04-01 00:00:00

abstract：PURPOSE:To evaluate the radioprotective activity of C3, a regioisomer of water-soluble carboxyfullerene and a potent free radical scavenger, on both normal and tumour cells. MATERIALS AND METHODS:The murine committed bone-marrow stem cells for both granulocytes and monocytes (GM-CFC) were used to represent normal cell...

journal_title：International journal of radiation biology

authors： Lin HS,Lin TS,Lai RS,D'Rosario T,Luh TY

更新日期：2001-02-01 00:00:00

abstract：:The kinetic scheme of the reaction of desferrioxamine (DFO) with O2-. was studied using pulse and gamma-radiolysis. The rate constant k(O2-. + DFO) is equal to 1.3 +/- 0.1 x 10(6) dm3 mol-1s-1 at pH 7.4. Studying the competition between DFO and ferricytochrome-c for O2-. generated by gamma-radiolysis, we observed that...

journal_title：International journal of radiation biology

authors： Sabourault D,Ribiere C,Nordmann R,Houee-Levin C,Ferradini C

更新日期：1989-12-01 00:00:00

abstract：PURPOSE:To determine whether cigarette smoke condensate (CSC) without metabolic activation induces direct DNA double strand breaks (DSB) in the G1 phase of various radiosensitive mutants of CHO cells and whether these breaks display collateral hypersensitivity to CSC with respect to cell killing. MATERIALS & METHODS:W...

journal_title：International journal of radiation biology

authors： Kato T,Nagasawa H,Warner C,Okayasu R,Bedford JS

更新日期：2007-09-01 00:00:00

abstract：PURPOSE:To investigate the influence of chromatin organization and scavenging capacity in relation to irradiation temperature on the induction of double-strand breaks (DSB) in structures derived from human diploid fibroblasts. MATERIALS AND METHODS:Agarose plugs with different chromatin structures (intact cells+/-wort...

journal_title：International journal of radiation biology

authors： Elmroth K,Nygren J,Stenerlöw B,Hultborn R

更新日期：2003-10-01 00:00:00

abstract：PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...

journal_title：International journal of radiation biology

authors： Liamsuwan T,Uehara S,Emfietzoglou D,Nikjoo H

更新日期：2011-02-01 00:00:00

abstract：PURPOSE:Biomarkers of ionising radiation exposure are useful in a variety of scenarios, such as medical diagnostic imaging, occupational exposures, and spaceflight. This study investigates to what extent microRNA (miRNA) expression signatures in mouse peripheral blood can be used as biomarkers for exposures to radiatio...

journal_title：International journal of radiation biology

authors： Templin T,Amundson SA,Brenner DJ,Smilenov LB

更新日期：2011-07-01 00:00:00

abstract：:During previous studies on the relationship between thermosensitivity and intracellular pH (pHi) regulation in mammalian cells, we observed that the thermoresistant TR-4 cells appeared to be resistant to manipulations of the Na+/H+ antiport, one of the two primary membrane regulators of pHi. We hypothesized that this ...

journal_title：International journal of radiation biology

authors： Liu FF,Lee C,Li JH

更新日期：1996-10-01 00:00:00

abstract：:Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0.1...

journal_title：International journal of radiation biology

authors： Lazutka JR,Dedonyte V

更新日期：1995-06-01 00:00:00

abstract：PURPOSE:Neutron irradiations at the McMaster Tandetron Accelerator were performed to study direct and bystander effects of neutrons in a live organism. METHODS:The neutrons were produced through (7)Li(p,n)(7)Be reaction. Although the gamma contamination of the neutron beam cannot be completely eliminated, it was desig...

journal_title：International journal of radiation biology

authors： Wang C,Smith RW,Duhig J,Prestwich WV,Byun SH,McNeill FE,Seymour CB,Mothersill CE

更新日期：2011-09-01 00:00:00

abstract：:The rate of recovery from radiation damage, as a function of dose per fraction, was investigated in mouse skin. Two different experimental designs were used, both incorporating the neutron top-up technique which enables the X-ray dose per fraction to be kept constant whilst changing the interfraction interval. Either ...

journal_title：International journal of radiation biology

authors： Rojas A,Joiner MC,Johns H

更新日期：1991-02-01 00:00:00

abstract：:Free radical formation in aqueous solutions of ubiquinone (coenzyme Qo) induced by ultrasound was studied by EPR and spin trapping with 3,5-dibromo-4-nitrosobenzene sulphonate. When aqueous solutions of ubiquinone were sonicated with 50-kHz ultrasound in the presence of argon or nitrogen, radicals formed by addition o...

journal_title：International journal of radiation biology

authors： Kondo T,Riesz P

更新日期：1996-01-01 00:00:00

abstract：:We have previously described a cell-free assay that can be employed to study rejoining of radiation-induced DNA double-stranded breaks (dsb) in 'naked' DNA prepared from agarose-embedded cells using an extract of HeLa cells as a source of enzymes. Rejoining of dsb in this assay is absolutely dependent on cell extract ...

journal_title：International journal of radiation biology

authors： Cheong N,Iliakis G

更新日期：1997-04-01 00:00:00

abstract：PURPOSE:The exploration of immune mechanisms of the tumour-inhibitory effect of exposures to low-level fractions of X-rays. MATERIALS AND METHODS:BALB/c mice were exposed to whole-body daily irradiations with 0.01, 0.02, or 0.1 Gy X-rays per day for 5 days/week for two weeks. Then, mice were intravenously injected wit...

journal_title：International journal of radiation biology

authors： Nowosielska EM,Cheda A,Wrembel-Wargocka J,Janiak MK

更新日期：2011-02-01 00:00:00

abstract：PURPOSE:Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. MATERIALS AND METHODS:Paddy field microcosm samples were continuously exposed at a dose rate o...

journal_title：International journal of radiation biology

authors： Ishii N,Fuma S,Tagami K,Honma-Takeda S,Shikano S

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:Global warming directly influencing ozone layer depletion, which eventually is increasing ultraviolet radiation penetration having far-reaching impacts on living biota. This particularly influences the primary producer microalgae which are the basic unit of food webs in the aquatic habitats. Therefore, it is...

journal_title：International journal of radiation biology

authors： El-Sheekh MM,Alwaleed EA,Ibrahim A,Saber H

更新日期：2020-12-03 00:00:00

abstract：:The aim was to review and summarize the results of 40 years of study concerning the response to ionizing radiation of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in sensitivity to various DNA-damaging agents, among them X- and gamma-rays. The reviewed data indicate the key importance of DNA damage rep...

journal_title：International journal of radiation biology

authors： Szumiel I

更新日期：2005-05-01 00:00:00

abstract：:This study was designed to compare radiosensitization by the oral platinum compound JM216 with cisplatin. RIF1 mouse tumour cells were treated at various doses and at various exposure times with JM216 and irradiated 15 min before the end of drug exposure. The fraction of cells surviving treatment was assessed by colon...

journal_title：International journal of radiation biology

authors： van de Vaart PJ,Klaren HM,Hofland I,Begg AC

更新日期：1997-12-01 00:00:00

abstract：PURPOSE:Design of cancer radiotherapy protocol to reduce radiation dose and increase treatment efficacy in Lewis lung cancer (LLC) model. METHODS:C57BL/6J mice subcutaneously implanted with LLC were treated by conventional radiotherapy (2Gy x 6) combined with LDWBI (low dose whole-body irradiation; the second, third, ...

journal_title：International journal of radiation biology

authors： Wu N,Jin SZ,Pan XN,Liu SZ

更新日期：2008-03-01 00:00:00

abstract：:The bacterial enzyme PvuII, which generates blunt-ended DNA double-strand breaks, and T4 DNA ligase, which seals adjacent DNA fragments in coupling to ATP cleavage, were introduced in mouse C3H10T1/2 fibroblasts using osmolytic shock of pinocytic vesicles. Cells were then assayed for their clonogenic ability. In agree...

journal_title：International journal of radiation biology

authors： Durante M,Grossi GF,Napolitano M,Gialanella G

更新日期：1991-04-01 00:00:00

abstract：:A new approach is reported that combines synchrotron radiolysis and mass spectrometry to probe the structure of proteins. Hydroxyl radicals produced upon the radiolysis of protein solutions using synchrotron light modify amino acid side-chains on millisecond timescales. This results in the formation of stable oxidatio...

journal_title：International journal of radiation biology

authors： Kiselar JG,Maleknia SD,Sullivan M,Downard KM,Chance MR

更新日期：2002-02-01 00:00:00

abstract：PURPOSE:To develop a reproducible single-cell gel electrophoresis assay for DNA damage and repair in buccal mucosa and sublingual exfoliated cells. MATERIALS AND METHODS:Buccal mucosa and sublingual cells and lymphocytes from six individuals (three males, three females, aged 34-45 years) were challenged with increasin...

journal_title：International journal of radiation biology

authors： Dhillon VS,Thomas P,Fenech M

更新日期：2004-07-01 00:00:00

abstract：PURPOSE:The actinides are chemical poisons and radiological hazards. One challenge to better appraise their toxicity and develop countermeasures in case of exposure of living organisms is to better assess pathways of contamination. Because of the high chemical affinity of those actinide elements for phosphate groups an...

journal_title：International journal of radiation biology

authors： Mostapha S,Fontaine-Vive F,Berthon L,Boubals N,Zorz N,Solari PL,Charbonnel MC,Den Auwer C

更新日期：2014-11-01 00:00:00

abstract：PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...

journal_title：International journal of radiation biology

authors： Sims AH,Finnon P,Miller CJ,Bouffler SD,Howell A,Scott D,Clarke RB

更新日期：2007-06-01 00:00:00

abstract：PURPOSE:Adverse effects on human health caused by electromagnetic fields (EMF) associated with the use of mobile phones, particularly among young people, are increasing all the time. The potential deleterious effects of EMF exposure resulting from mobile phones being used in close proximity to the brain require particu...

journal_title：International journal of radiation biology

authors： Erdem Koç G,Kaplan S,Altun G,Gümüş H,Gülsüm Deniz Ö,Aydin I,Emin Onger M,Altunkaynak Z

更新日期：2016-10-01 00:00:00