Abstract:
PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-15 cell-mediated activation of JB4 CTL in terms of interferon (IFN)-gamma secretion and cytotoxic potential was determined by enzyme-linked immunosorbent assay and chromium release assay, the perforin content of JB4 cells by flow cytometry. In vivo, tumours were irradiated with 14 Gy. Subsequently, JB4 CTL were injected intra- and peritumourally. Volume doubling times were calculated as a marker of tumour growth delay. RESULTS:UT-SCC-15 tumour cells were well recognized by JB4 CTL in vitro, as indicated by profound IFN-gamma secretion and tumour cell lysis. This response was completely abrogated in the presence of an anti-HLA-A2 antibody. In vivo, adoptive transfer of JB4 CTL after irradiation did not delay tumour growth in comparison to irradiation alone. As a possible underlying mechanism, a loss of perforin content and cytolytic function of the CTL in the absence of interleukin (IL)- 2 or IL-15 was found in vitro. CONCLUSION:HLA-A2-alloreactive JB4 cells efficiently recognize and destroy UT-SCC-15 tumour cells in vitro. However, the intratumoural application of JB4 cells after irradiation does not enhance the in vivo effect of radiotherapy alone, which might be caused by the reduced cytotoxic potential of JB4 cells in the absence of IL-2 or IL-15. Thus, co-administration of these cytokines might improve the efficacy of combined irradiation and CTL treatment.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Krause M,Schmitz M,Noessner E,Skrablin PS,Wehner R,Rieber EP,Baumann Mdoi
10.1080/09553000701570196subject
Has Abstractpub_date
2007-11-01 00:00:00pages
827-36issue
11-12eissn
0955-3002issn
1362-3095pii
781645177journal_volume
83pub_type
杂志文章abstract::Induction of mutations to 6-thioguanine resistance (TGr) by gamma-rays at three different dose-rates and molecular changes in the HPRT gene were studied in human lymphoblastoid WIL2-NS cells. Mutant induction showed a curvilinear dose-response for acute irradiation (30 Gy/h). The total mutant frequency was lower after...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145201
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abstract:PURPOSE:The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity...
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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abstract:PURPOSE:To study the natural radionuclides in the freshwater fish samples around the uranium mining areas of Bagjata and Banduhurang, East Singhbhum, Jharkhand, India. MATERIALS AND METHODS:The naturally occurring radioisotopes of uranium, U(nat), consisting of (234)U, (235)U and (238)U; (226)Ra, (230)Th and (210)Po w...
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abstract:PURPOSE:To investigate the possible role of cell cycle arrest in the radiosensitization of mouse spermatogonial stem cells due to small conditioning X-ray exposures. MATERIALS AND METHODS:A 24 h fractionation interval between conditioning (1 Gy) and challenging (8 or 9 Gy) exposures was used. Two approaches were follo...
journal_title:International journal of radiation biology
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abstract::The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title:International journal of radiation biology
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更新日期:1997-07-01 00:00:00
abstract:PURPOSE:Testicular seminoma cancer incidence has significantly increased over the last few decades, and although it is successfully treated by radiotherapy, long-term health risks are still unclear. The aim of the study was to show long-term genome damage in patients with seminoma after radiotherapy. MATERIALS AND MET...
journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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更新日期:2004-05-01 00:00:00
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abstract::A/J and C57BL/6J mice, strains which differ in susceptibility to chemical-induced lung tumours, were examined for differential sensitivity to radon-induced lung cancer. Sixty weeks after exposure to 952 WLM radon (3.34 J h1 m-3), no lung tumours were found in the control, sham- (one dust-) exposed, or radon-exposed C5...
journal_title:International journal of radiation biology
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abstract:PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...
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abstract:PURPOSE:To investigate the effect of lupeol, a pentacyclictriterpene, on the radiosensitivity of a human hepatocellular carcinoma (HCC) in vitro and in vivo xenografts. METHODS:SMMC-7721 cells were exposed to γ-radiation with or without lupeol and assayed for proliferation, clonogenic survival, apoptosis and cell cycl...
journal_title:International journal of radiation biology
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更新日期:2015-02-01 00:00:00
abstract:PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...
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更新日期:2007-06-01 00:00:00
abstract:PURPOSE:To investigate the induction and persistence of chromosome aberrations in mouse bone marrow cells after X-ray exposure and to detect differential involvement of individual chromosomes in translocations. MATERIALS AND METHODS:Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome aberration...
journal_title:International journal of radiation biology
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doi:10.1080/095530099139593
更新日期:1999-09-01 00:00:00
abstract:PURPOSE:The space environment contains two major biologically significant influences; space radiations and microgravity. The 53 kDa tumour suppressor protein (p53) plays a role as a guardian of the genome through the activity of p53-centered signal transduction pathways. The aim of this study was to clarify the biologi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003789596
更新日期:2010-08-01 00:00:00
abstract::In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144860
更新日期:1996-10-01 00:00:00
abstract:PURPOSE:To investigate the mechanisms inducing and maintaining the permanent elimination of low dose hyper-radiosensitivity (HRS) in cells given a dose of 0.3 Gy at low dose-rate (LDR) (0.3 Gy/h). MATERIALS AND METHODS:Two human HRS-positive cell lines (T-47D, T98G) were used. The effects of pretreatments with transfo...
journal_title:International journal of radiation biology
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doi:10.3109/09553002.2014.906767
更新日期:2014-07-01 00:00:00
abstract:PURPOSE:To develop and validate non-fluorescent chromosome painting for bright-field microscopy using the peroxidase/diaminobenzidine (DAB) reaction. MATERIALS AND METHODS:Peripheral blood lymphocytes were taken from patients with uterine cancer who had received heavy-ion radiation therapy. Chromosome slides were trea...
journal_title:International journal of radiation biology
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doi:10.1080/095530098142077
更新日期:1998-05-01 00:00:00
abstract:PURPOSE:To investigate cell cycle effects and relative biological effectiveness (RBE) of α-particles from the clinically relevant radionuclide Astatine-211 ((211)At), using X-rays as reference radiation. Double-strand breaks (DSB), non-DSB clusters containing oxidised purines and clonogenic survival were investigated. ...
journal_title:International journal of radiation biology
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更新日期:2011-04-01 00:00:00
abstract::The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550601
更新日期:1994-05-01 00:00:00
abstract::Solid human tumours contain areas with low oxygen tension (pO2). For bioreductive drugs it is important to define the cytotoxic effect according to drug concentration and to clinically relevant pO2. In this study, the pO2 dependence of the survival of three human cell lines (HRT 18, Na11 +, and MEWO), exposed to tirap...
journal_title:International journal of radiation biology
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更新日期:1995-02-01 00:00:00
abstract:PURPOSE:To investigate the induction of chromosomal aberrations in mouse m5S cells after exposure to power-line frequency magnetic fields (extremely low frequency magnetic fields; ELFMF) at high-flux densities. MATERIAL AND METHOD:m5S cells were either untreated or pretreated during the G1 phase with mitomycin C (MMC,...
journal_title:International journal of radiation biology
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doi:10.1080/09553000050201172
更新日期:2000-12-01 00:00:00
abstract::The in vitro cell survival of a human cervix carcinoma cell line (HX156c) has been assessed using 60Co gamma-rays administered at either high (150 cGy/min) or low (3.2 cGy/min) dose rate. Recovery during low dose-rate irradiation was observed; the dose reduction factor at 10(-2) cell kill for 150 versus 3.2 cGy/min wa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551671
更新日期:1988-08-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138600
更新日期:2000-04-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:2002-03-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141771
更新日期:1998-07-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552201
更新日期:1988-11-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.698366
更新日期:2012-08-01 00:00:00
abstract::The recently released data on cancer incidence in the Japanese atomic bomb survivors are analysed using a variety of relative risk models which take account of errors in estimates of dose to assess the dose-response at low doses. For all solid cancers analysed together there is a significant positive dose-response (at...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145364
更新日期:1996-07-01 00:00:00