Abstract：
:The relationship between cellular radiosensitivity and normal-tissue response to radiotherapy in individual cancer patients has attracted increasing attention over the last few years. Recent work has suggested that a correlation exists between fibroblast sensitivity and normal-tissue reactions. We have examined the radiosensitivity of fibroblasts grown from skin biopsies of four normal individuals and three patients identified as having suffered unexpectedly severe reactions to clinical radiotherapy, called here 'over-reactor' (OR) patients. Clonogenic survival was measured after high (HDR) and low dose-rate (LDR) irradiation. By comparing the two, and LDR Recovery Factor was derived. Potentially-lethal damage repair was examined in 4 cell strains. After HDR the OR strains were indistinguishable from the normals. At LDR the range of sensitivity was expanded. The OR strains fell at the sensitive end of the range and were characterized by a lack of LDR recovery, which clearly distinguished them from the normal strains. Experimental errors were estimated by considering all the data sets together rather than viewing each experiment individually. Duplicate strains from several patients were tested, and the differences between them were found to be within the estimated experimental errors, suggesting that these differences were not biologically significant. The data are consistent with the hypothesis that normal-tissue response is linked to individual cellular radiosensitivity. Our data confirm the importance of using LDR irradiation in clinical investigations of cellular sensitivity.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Burnet NG,Wurm R,Peacock JHdoi
10.1080/095530096145021subject
Has Abstractpub_date
1996-09-01 00:00:00pages
289-300issue
3eissn
0955-3002issn
1362-3095journal_volume
70pub_type
杂志文章abstract：:Purpose: To summarize existing knowledge and to understand individual response to radiation exposure, the MELODI Association together with CONCERT European Joint Programme has organized a workshop in March 2018 on radiation sensitivity and susceptibility.Methods: The workshop reviewed the current evidence on this matt...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2019.1665209
更新日期：2020-03-01 00:00:00
abstract：PURPOSE:The tumour suppressor protein p53 is considered to have an impact on the radiosensitivity of tumour cells. However, this concept does not easily translate to the tumour sensitivity in the clinics. The aim of the present study was to determine whether a functional or dysfunctional p53 is associated with a sensit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001642902
更新日期：2004-01-01 00:00:00
abstract：PURPOSE:To investigate whether mobile phone radiation might affect snail nociception, employing radiofrequency (RF) electromagnetic fields (EMF) which, to our knowledge, have hitherto not been studied in a snail model. Exposure to extremely low frequency (ELF) magnetic fields has however been shown to significantly aff...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.644257
更新日期：2012-03-01 00:00:00
abstract：PURPOSE:This study was undertaken to understand the roles of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of the process following exposure of E. coli to gamma-radiation in vivo. MATERIALS AND METHODS:A plasmid DNA construct, pMTa4, was transformed into isogenic repair proficient (wild)...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000601121140
更新日期：2007-02-01 00:00:00
abstract：:The role of protein kinase C in radiation-induced death of thymocytes was studied. For this purpose murine thymocytes were irradiated and incubated for 6 h at 37 degrees C and afterwards the fraction of fragmented DNA was measured. Results indicate that radiation-induced DNA fragmentation can be prevented by adding th...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551471
更新日期：1992-05-01 00:00:00
abstract：PURPOSE:This review will examine the role of phosphatases in cancer cell signalling and also outline emerging findings regarding the influence of phosphatases on tumor cell survival after ionising radiation. CONCLUSION:The exposure of tumour cells to clinically relevant doses of ionising radiation causes DNA damage an...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.3109/09553000903232827
更新日期：2009-11-01 00:00:00
abstract：PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110075220
更新日期：2001-12-01 00:00:00
abstract：:The literature suggests that the function of radiation-induced G2 arrest is to allow repair of potentially lethal damage before cell-entry into, and damage expression in, mitosis. The nature of the damage repaired is not known, but chromosome aberrations have been considered. To examine this possibility in G2 cells, t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551831
更新日期：1990-09-01 00:00:00
abstract：PURPOSE:We introduce and evaluate a high throughput biodosimetry test system (REDI-Dx) suitable for testing of thousands of potential radiation victims following a mass scale nuclear event caused by detonation of a nuclear device or a nuclear accident, as part of an overall strategy for effective medical management of ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2018.1532615
更新日期：2020-01-01 00:00:00
abstract：:237Np nitrate was injected intravenously into 4-week-old (young) and 10-12-week-old (adult) female albino Sprague-Dawley rats. The amounts given were 52 (young), 5.2 (adult) and 26 kBq kg-1 body weight (adult). The microscopic distribution in the femur and the lumbar vertebrae was studied. Initially, neptunium was dis...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550511
更新日期：1993-03-01 00:00:00
abstract：:We have measured clonal survival following gamma-irradiation of unstimulated (G0) T-lymphocytes from 35 donors, of 11 T-lymphocyte cell lines, of six lymphoblastoid cell lines, and of nine primary fibroblast strains for which we have G0 T-lymphocyte material from the same donor. Amongst the G0 lymphocytes we have resu...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814552331
更新日期：1988-12-01 00:00:00
abstract：:Cell cycle dependence for the induction of 6-thioguanine-resistant mutation was investigated using synchronized mouse L5178Y cells after exposure to 4 Gy to 60Co gamma-rays or 1 Gy of 252Cf radiation (fission neutrons). Maximal mutation frequency was observed immediately after release from colcemid block (G2/M phase) ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550631
更新日期：1993-04-01 00:00:00
abstract：:Samples of human rib were collected at autopsy and 20 were selected for marrow fat cell measurement, representing an age range of 16-96 years. The mean diameter of fat cells in red bone marrow of human rib was found to increase from around 48 microns at ages 16-29 years to around 65 microns at ages 82-96 years. There ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551681
更新日期：1995-12-01 00:00:00
abstract：:The existence of a correlation between experimental probabilities of cell inactivation by charged particles and calculated probabilities for K-vacancy production in heavy atoms (C, N, O, P) of the DNA of cell nuclei is established. Both phenomena display a similar dependence upon the linear energy transfer (LET) of in...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414550601
更新日期：1994-05-01 00:00:00
abstract：:The aim was to identify the decorporation and anti-oxidation efficacy of prompt and delayed consecutive administration of the catecholicpolyaminopolycarboxylate ligands 9501 and 7601 for radiothorium in vivo. The chelating agents 9501 or 7601 were administered intramuscularly to ICR mice 3 min or 3 days after intraper...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500141124
更新日期：2005-04-01 00:00:00
abstract：:The resonant recognition model (RRM) is a model of protein-protein and protein-DNA interaction based on a significant correlation between spectra of numerical presentation of the amino acid or nucleotide sequences and their biological activity. Having compared absorption characteristics of photosensitive proteins with...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914551331
更新日期：1989-08-01 00:00:00
abstract：:Calibration of the non-denaturing (pH 9.6) filter elution technique by 125I decays has been used to examine the relationship between X-ray-induced DNA double-strand breaks (dsb) and cellular survival of CHO cells and human fibroblasts. DNA dsb induction varies markedly between the two cell lines, reflecting the differ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550411
更新日期：1993-03-01 00:00:00
abstract：:Free radical formation in aqueous solutions of ubiquinone (coenzyme Qo) induced by ultrasound was studied by EPR and spin trapping with 3,5-dibromo-4-nitrosobenzene sulphonate. When aqueous solutions of ubiquinone were sonicated with 50-kHz ultrasound in the presence of argon or nitrogen, radicals formed by addition o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096146246
更新日期：1996-01-01 00:00:00
abstract：PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.809170
更新日期：2013-11-01 00:00:00
abstract：PURPOSE:The actinides are chemical poisons and radiological hazards. One challenge to better appraise their toxicity and develop countermeasures in case of exposure of living organisms is to better assess pathways of contamination. Because of the high chemical affinity of those actinide elements for phosphate groups an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.886795
更新日期：2014-11-01 00:00:00
abstract：:Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately t...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553008914551971
更新日期：1989-11-01 00:00:00
abstract：PURPOSE:To test whether radiolysis-induced fragmentation in frozen aqueous protein solution is dependent on solvent access to the surface of the protein or to the molecular mass of the polypeptide chain. MATERIALS AND METHODS:60Co gamma-irradiation of three proteins at -78 degrees C: lysozyme, citrate synthase and alp...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138349
更新日期：2000-05-01 00:00:00
abstract：:A novel 2-nitroimidazole with a theophylline side-chain, 7-(4'-(2-nitroimidazol-1-yl)-butyl)-theophylline, (NITP) was as efficient a hypoxic-cell radiosensitizer as misonidazole. However, if cells were irradiated with NITP under hypoxic conditions and then exposed to the drug under aerobic conditions, a much larger ra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551671
更新日期：1992-06-01 00:00:00
abstract：PURPOSE:The extreme radiotoxicity of Auger electrons and their exquisite capacity to irradiate specific molecular sites has prompted scientists to extensively investigate their radiobiological effects. Their efforts have been punctuated by quadrennial international symposia that have focused on biophysical aspects of A...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000802395527
更新日期：2008-12-01 00:00:00
abstract：:The effect of gamma-radiation on aqueous solutions of saturated phospholipids, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (DPPG), 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine (lysoPC), and bovine brain sphingomyelin (SM) has been investigated. It is shown that th...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143888
更新日期：1997-05-01 00:00:00
abstract：:Pulsed-field electrophoresis is being used extensively in the gene mapping studies and in the analysis of DNA strand breakage by ionizing radiation. We have evaluated the relationship between the fraction of S phase DNA in a cell population and its ability to modify the migration of DNA in pulsed-field gel electrophor...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145427
更新日期：1996-06-01 00:00:00
abstract：PURPOSE:The LET position of the RBE maximum and its dependence on the cellular repair capacity was determined for carbon ions. Hamster cell lines of differing repair capacity were irradiated with monoenergetic carbon ions. RBE values for cell inactivation at different survival levels were determined and the differences...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139232
更新日期：1999-11-01 00:00:00
abstract：:We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551211
更新日期：1992-04-01 00:00:00
abstract：:Cell death induced by various radiation doses in Molt-4 and L5178Y cells was studied. Molt-4 cells have a low threshold for induction of interphase death while L5178Y cells have a high threshold. Interphase death in Molt-4 cells at doses > 100 Gy and in L5178Y cells at doses > 300 Gy showed typical necrosis. In contra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551101
更新日期：1993-07-01 00:00:00
abstract：:Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551111
更新日期：1990-05-01 00:00:00
