Abstract：
:The radiosensitization of two human tumour in vitro cell lines, HT-1080 and LoVo, has been compared with that of the Chinese hamster cell line V79-379A. Although the two human tumour cell lines were more radiosensitive than the V79 cell line sensitizer, enhancement ratios for misonidazole, pimonidazole and azomycin were similar (relative to extracellular concentration) for all three cell lines. Average intracellular concentrations of radiosensitizer were measured by high-performance liquid chromatography. In all three cell lines the uptake of misonidazole and azomycin was extremely rapid whereas that of pimonidazole was initially much slower before reaching a plateau. The ratios of intracellular concentration of radiosensitizer to extracellular concentration (Ci to Ce) for misonidazole were 0.8 (HT-1080) and 0.7 (LoVo and V79); for azomycin 0.9 (HT-1080 and LoVo) and 0.8 (V79). In contrast Ci/Ce for pimonidazole varied with cell line, the values being 1.8 (LoVo), 2.6 (HT-1080) and 3.3 (V79). Intracellular amounts of non-protein sulphydryl (NPSH) varied between cell lines by about a factor of three. However, when the average cell volume was taken into consideration the concentrations of NPSH were very similar, being 4.2 (HT-1080), 5.6 (LoVo) and 5.7 (V79) mmol dm-3. NPSH levels expressed as nmol per mg protein were also similar.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Watts ME,Dennis MF,Roberts IJdoi
10.1080/09553009014552461subject
Has Abstractpub_date
1990-02-01 00:00:00pages
361-72issue
2eissn
0955-3002issn
1362-3095pii
6QQ35QWT0RQLV44Djournal_volume
57pub_type
杂志文章abstract：PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2017.1328750
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:5-Formyluracil (5-foU) is a major derivative of thymine produced in DNA by ionizing radiation and various chemical oxidants. It has been previously shown that 5-foU in template DNA directs misincorporation of nucleotides by DNA polymerases during in vitro DNA synthesis. The present experiments were designed to ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530001453113
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:To evaluate how irradiation affects thyroid autoimmunity in mouse models of Hashimoto's thyroiditis and Graves' hyperthyroidism. MATERIALS AND METHODS:Non-obese diabetic (NOD)-H2(h4) mice spontaneously develop anti-thyroglobulin (Tg) antibodies and thyroiditis when supplied with sodium iodine (NaI) in the drin...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802345910
更新日期：2008-09-01 00:00:00
abstract：:The effect of local stimulation on mitotic activity and radiation tolerance was studied in mouse tongue mucosa. Silver nitrate solution (0.5-20%) was used for local conditioning. The most effective protocol comprised three daily treatments (days 0-2), yielding a delayed increase in 24 h mitotic counts by about 30% on ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214551061
更新日期：1992-03-01 00:00:00
abstract：:Genetic analysis was performed on 72 Italian A-T families ascertained through 91 probands. The frequency of the A-T gene was estimated through Dahlberg's formula, which uses the frequencies of first cousin marriages among the patients' parents and in the general population were obtained from the collection of Catholic...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：PURPOSE:Theoretical modelling techniques are often used to simulate the action of ionizing radiations on cells at the nanometre level. Using monoenergetic vacuum-UV (VUV) radiation to irradiate DNA either dry or humidified, the action spectra for the induction of DNA damage by low energy photons and the role of water a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050028913
更新日期：2000-06-01 00:00:00
abstract：PURPOSE:Following ionizing radiation exposure, intestinal crypt regeneration is possible but it is still not known if regenerating crypts give rise to differentiated functional epithelial cells on villi. The aim of this study was to demonstrate that irradiated progeny of enterocytic precursor cells are capable of proli...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500091923
更新日期：2005-03-01 00:00:00
abstract：:Murine bone marrow contains osteogenic precursor cells that undergo differentiation during in vitro cultivation. In vitro these cells are potential target cells for alpha-irradiation-induced bone tumour formation. Under defined tissue culture conditions these differentiating cells were directly exposed to alpha-partic...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551111
更新日期：1994-08-01 00:00:00
abstract：PURPOSE:To develop a procedure, using the inverse polymerase chain reaction, to detect and sequence ionizing radiation-induced DNA rearrangements without prior phenotypic selection of mutant cells. METHOD:Normal human fibroblast cells (IMR-90) were given 30Gy of gamma-irradiation and then incubated at 37 degrees C for...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141672
更新日期：1998-07-01 00:00:00
abstract：PURPOSE:Chironomus ramosus is one of the recently reported radiotolerant insects. Salivary gland cells of fourth instar larvae respond to ionizing radiations with increases in the levels of antioxidant enzymes and chaperone proteins. Here we made an attempt to study the state of nuclear DNA after exposure of larvae to ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1062572
更新日期：2015-01-01 00:00:00
abstract：:Wet heat shock (60 degrees C, 90 s) and caffeine (3.8 X 10(-4) M) afford significant radioprotection against post-irradiation O2-dependent damage which develops in seeds of approximately 3.5% moisture content. The damage was assessed in terms of seedling injury on the eighth day of growth. An increase in seedling inju...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114551101
更新日期：1991-05-01 00:00:00
abstract：PURPOSE:To study the formation and subsequent reactions of the 5-methyl-2'-deoxycytidine cation radical (5-Me-2'-dC•(+)) in nucleosides and DNA-oligomers and compare to one-electron oxidized thymidine. MATERIALS AND METHODS:Employing electron spin resonance (ESR), cation radical formation and its reactions were invest...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.884293
更新日期：2014-06-01 00:00:00
abstract：:Purpose: To evaluate the antioxidant and radio-protective effects of Nigella sativa oil (NSO) and thymoquinone (TQ) on radiation-induced oxidative stress in brain tissue.Materials and methods: Fifty-four Sprague-Dawley rats were divided into six groups to test the radio-protective effectiveness of Nigella sativa oil a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1683636
更新日期：2020-02-01 00:00:00
abstract：:Existing models of chromosome aberrations produced by ionizing-radiation predict equal numbers of dicentrics and translocations if the dose is so low that complex aberrations can be ignored. We show that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at highe...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145977
更新日期：1996-02-01 00:00:00
abstract：:Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551111
更新日期：1990-05-01 00:00:00
abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552611
更新日期：1990-03-01 00:00:00
abstract：:Photoirradiation of aqueous solutions of DNA in the presence of Al- or Zn-tetrasulphonated phthalocyanines (AIPcS4 and ZnPcS4) causes formation of strand breaks and liberation of nucleobases. The effect of added D2O, which enhances singlet oxygen (1O2) lifetime, radical scavengers including alcohols and the spin-trap ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:I calculate the probability that a single high-energy ionizing particle or photon makes two widely spaced double-strand breaks in the same DNA molecule. Deletions (or inversions) between two breaks formed by the same incident particle are linear in radiation dose and occur even at extremely low dose-rates; deletions b...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144699
更新日期：1996-11-01 00:00:00
abstract：:Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scorin...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314552131
更新日期：1993-06-01 00:00:00
abstract：:We characterized in vitro the effects of gamma-radiation (12.5-100 cGy) on pulmonary microvascular endothelial cell (PMEC) morphology and F-actin organization. Cellular retraction was documented by phase-contrast microscopy and the organization of actin microfilaments was determined by immunofluorescence. Characteriza...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551471
更新日期：1993-09-01 00:00:00
abstract：PURPOSE:Terrorist attacks, with their intent to maximize psychological and economic damage as well as inflicting sickness and death on given targeted populations, are an ever-growing worldwide concern in government and public sectors as they become more frequent, violent, and sensational. If given the chance, it is lik...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2017.1332440
更新日期：2017-09-01 00:00:00
abstract：:Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, a multiscale high-throughput multi-omics analysis was applied with a protocol for chronic exposure to the rat kidney. Methods: Male and female rats were contaminated for nine months through their drinking water with a nont...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1577567
更新日期：2019-06-01 00:00:00
abstract：PURPOSE:Since the number of cancer patients treated by proton irradiation has increased in the last few years, it seems appropriate to study dose-dependent effects of proton irradiation on mammalian tissues in more detail. MATERIALS AND METHODS:Tissue samples of normal skin of mouse and swine, of a human tumour model ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000412331283515
更新日期：2004-08-01 00:00:00
abstract：PURPOSE:To ask whether highly metastatic sublines show more marked low-dose hyper-radiosensitivity (HRS) response than poorly metastatic ones. MATERIALS AND METHODS:The progressive (PRO) subline showing tumourigenicity and metastatic potential and the regressive (REG) subline showing neither tumourigenicity nor metast...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802195331
更新日期：2008-07-01 00:00:00
abstract：:This paper presents a summary of our understanding to date of the formation of DNA strand breaks induced by highly energetic particle beams (high-LET radiation). We have compared our own recent data on the formation of strand breaks induced in DNA in an aqueous solution with our previous data and those of others avail...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014551431
更新日期：1990-07-01 00:00:00
abstract：PURPOSE:To test whether radiolysis-induced fragmentation in frozen aqueous protein solution is dependent on solvent access to the surface of the protein or to the molecular mass of the polypeptide chain. MATERIALS AND METHODS:60Co gamma-irradiation of three proteins at -78 degrees C: lysozyme, citrate synthase and alp...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138349
更新日期：2000-05-01 00:00:00
abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009414551661
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:The sequelae of premature loss of ovarian function can undoubtedly have undesirable effects for a woman although radiotherapy is one of the most relevant treatment modalities for various types of malignancies. The aim of this study was to determine the effect of different doses of radiation on ovarian folliculo...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1864497
更新日期：2021-01-06 00:00:00
abstract：BACKGROUND:Element-specific biokinetic models are used to reconstruct doses to systemic tissues from internal emitters. Typically, a systemic model for a radionuclide explicitly depicts only its dominant repositories. Remaining tissues and fluids are aggregated into a pool called Other tissue in which the radionuclide ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2018.1554923
更新日期：2018-12-04 00:00:00
abstract：PURPOSE:Primary fibroblasts are not suitable for in vitro macrocolony assay due to their inability to form distinct colonies. Here we present a modification of agarose overlay that yielded extensive improvement in their colony formation and assessment of radiosensitivity. MATERIALS AND METHODS:Macrocolony formation wa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.894650
更新日期：2014-05-01 00:00:00
