Biochemical effects of heat shock and caffeine on post-irradiation oxic and anoxic damage in barley seeds of low and high water content.


:Wet heat shock (60 degrees C, 90 s) and caffeine (3.8 X 10(-4) M) afford significant radioprotection against post-irradiation O2-dependent damage which develops in seeds of approximately 3.5% moisture content. The damage was assessed in terms of seedling injury on the eighth day of growth. An increase in seedling injury is clearly seen, associated with a parallel increase in the peroxidase activity. There is a concomitant decrease in the content of total peroxides. Both these post-irradiation treatments potentiate the O2-independent component of seedling injury, irrespective of the seed moisture content. Analysis of the peroxidase activity in the seedlings using non-denaturing polyacrylamide gel electrophoresis reveals that two additional bands appear with the post-irradiation oxic damage. Radioprotection against this damage by caffeine, heat shock and O2-free post-irradiation hydration is accompanied by the disappearance of these two additional bands. However there is no appearance of the two additional bands in the peroxidase family even though the enzyme activity is substantially increased due to the action of caffeine and/or heat shock on the post-irradiation O2-independent pathway of damage. The probable mechanisms of radioprotection are discussed in this paper.


Int J Radiat Biol


Singh SP,Kesavan PC




Has Abstract


1991-05-01 00:00:00














  • Effects of in vitro alpha-particle irradiation on osteogenic bone marrow cultures.

    abstract::Murine bone marrow contains osteogenic precursor cells that undergo differentiation during in vitro cultivation. In vitro these cells are potential target cells for alpha-irradiation-induced bone tumour formation. Under defined tissue culture conditions these differentiating cells were directly exposed to alpha-partic...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Schoeters GE,Vander Plaetse F,van den Heuvel RL

    更新日期:1994-08-01 00:00:00

  • International coordination of research: EPRI International Dose Effect Alliance.

    abstract::Purpose: The key to increasing our understanding of low dose effects of ionizing radiation is cooperation and communication between research organizations around the world. The purpose of this article is to describe the Electric Power Research Institute (EPRI) program for such cooperation. Materials and methods: EPRI ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cool DA

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  • Increased frequency of sister chromatid exchanges in lymphocytes of Chernobyl clean-up workers.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Lazutka JR,Dedonyte V

    更新日期:1995-06-01 00:00:00

  • The relevance of tumour and surrounding normal tissue vascular density in clinical hyperthermia of locally advanced breast carcinoma.

    abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Lyng H,Monge OR,Bøhler PJ,Rofstad EK

    更新日期:1991-07-01 00:00:00

  • Different radiosensitivity of CD4(+)CD25(+) regulatory T cells and effector T cells to low dose gamma irradiation in vitro.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Cao M,Cabrera R,Xu Y,Liu C,Nelson D

    更新日期:2011-01-01 00:00:00

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    authors: Oppitz U,Schulte S,Stopper H,Baier K,Müller M,Wulf J,Schakowski R,Flentje M

    更新日期:2002-07-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Aypar U,Morgan WF,Baulch JE

    更新日期:2011-02-01 00:00:00

  • Accumulation of spermidine/spermine N1-acetyltransferase and alternatively spliced mRNAs as a delayed response of HeLa S3 cells following X-ray irradiation.

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    pub_type: 杂志文章


    authors: Mita K,Fukuchi K,Hamana K,Ichimura S,Nenoi M

    更新日期:2004-05-01 00:00:00

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    authors: Morris MD,Jones TD

    更新日期:1989-03-01 00:00:00

  • Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field.

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    authors: Dinčić M,Krstić DZ,Čolović MB,Nešović Ostojić J,Kovačević S,De Luka SR,Djordjević DM,Ćirković S,Brkić P,Todorović J

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  • Repair of DNA strand breaks at hyperthermic temperatures in Chinese hamster ovary cells.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Warters RL,Axtell J

    更新日期:1992-01-01 00:00:00

  • Chironomus ramosus larvae exhibit DNA damage control in response to gamma radiation.

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    更新日期:2015-01-01 00:00:00

  • BRCA1 and BRCA2 heterozygosity and repair of X-ray-induced DNA damage.

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    更新日期:2002-04-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Müller WU,Streffer C,Niedereichholz F

    更新日期:1992-08-01 00:00:00

  • Increased mutability and decreased repairability of a three-lesion clustered DNA-damaged site comprised of an AP site and bi-stranded 8-oxoG lesions.

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    journal_title:International journal of radiation biology

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    更新日期:1998-01-01 00:00:00

  • RBE for carbon track-segment irradiation in cell lines of differing repair capacity.

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    journal_title:International journal of radiation biology

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    authors: Weyrather WK,Ritter S,Scholz M,Kraft G

    更新日期:1999-11-01 00:00:00

  • Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes.

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    journal_title:International journal of radiation biology

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    authors: Schmid E,Zitzelsberger H,Braselmann H,Gray JW,Bauchinger M

    更新日期:1992-12-01 00:00:00

  • Influence of low doses of gamma-irradiation on mouse skin collagen fibrils.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Tzaphlidou M,Kounadi E,Leontiou I,Matthopoulos DP,Glaros D

    更新日期:1997-01-01 00:00:00

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    journal_title:International journal of radiation biology

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    authors: Richardson RB

    更新日期:2008-10-01 00:00:00

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    authors: Terrissol M,Edel S,Pomplun E

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Akiyama M

    更新日期:1995-11-01 00:00:00

  • The long-term effects of γ-radiation on the growth of Allium cepa plants.

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    journal_title:International journal of radiation biology

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    authors: Bolsunovsky A,Trofimova E,Dementyev D,Petrichenkov M

    更新日期:2020-11-16 00:00:00

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    authors: Griffin CS,Neshasateh-Riz A,Mairs RJ,Wright EG,Wheldon TE

    更新日期:2000-07-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Galvin JB,Bice DE,Guilmette RA,Muggenburg BA,Haley PJ

    更新日期:1989-02-01 00:00:00

  • Radiation-induced DNA double-strand break frequencies in human squamous cell carcinoma cell lines of different radiation sensitivities.

    abstract::DNA neutral (pH 9.6) filter elution was used to measure radiation-induced DNA double-strand break (dsb) frequencies in eight human squamous cell carcinoma cell lines with radiosensitivities (D0) ranging from 1.07 to 2.66 Gy and D values ranging from 1.46 to 4.08 Gy. The elution profiles of unirradiated samples from mo...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Schwartz JL,Mustafi R,Beckett MA,Czyzewski EA,Farhangi E,Grdina DJ,Rotmensch J,Weichselbaum RR

    更新日期:1991-06-01 00:00:00

  • Preferential tumour accumulation of gold nanoparticles, visualised by Magnetic Resonance Imaging: radiosensitisation studies in vivo and in vitro.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Hébert EM,Debouttière PJ,Lepage M,Sanche L,Hunting DJ

    更新日期:2010-08-01 00:00:00

  • Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry.

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Grégoire E,Roy L,Buard V,Delbos M,Durand V,Martin-Bodiot C,Voisin P,Sorokine-Durm I,Vaurijoux A,Voisin P,Baldeyron C,Barquinero JF

    更新日期:2018-03-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Kantak SS,Diglio CA,Onoda JM

    更新日期:1993-09-01 00:00:00

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    journal_title:International journal of radiation biology

    pub_type: 杂志文章


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    更新日期:2003-04-01 00:00:00