Abstract：
PURPOSE:Neutron irradiations at the McMaster Tandetron Accelerator were performed to study direct and bystander effects of neutrons in a live organism. METHODS:The neutrons were produced through (7)Li(p,n)(7)Be reaction. Although the gamma contamination of the neutron beam cannot be completely eliminated, it was designed to be as low as possible and remain below a threshold already established for bystander effects. Microdosimetric methods using a tissue-equivalent proportional counter have been used to measure the neutron and gamma doses for the cell irradiation. Previous data for a cell line exposed in vitro suggested that neutrons did not produce bystander effects at doses below 300 mGy. The current experiments sought to confirm this using a live whole organism (zebrafish) where tissue samples harvested 2 h after exposure were examined for direct evidence of apoptosis and tested for secretion of bystander factors using an established bioassay. Fish were either exposed directly to the beam or were allowed to swim with or in water previously occupied by irradiated fish. RESULTS:Using the zebrafish model it was found that there was significant direct cell death seen both by apoptosis scores and clonogenic assay when the neutron dose was approximately 100 mGy. An equivalent dose of gamma rays produced a more toxic effect. It was further found that neutrons did not induce a bystander effect in fish receiving signals from irradiated fish. CONCLUSION:The results confirm in vitro experiments which suggest neutrons do not induce bystander signaling. In fact they may suppress gamma induced signaling suggesting a possible intriguing new and as yet unclear mechanism.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Wang C,Smith RW,Duhig J,Prestwich WV,Byun SH,McNeill FE,Seymour CB,Mothersill CEdoi
10.3109/09553002.2011.584939subject
Has Abstractpub_date
2011-09-01 00:00:00pages
964-73issue
9eissn
0955-3002issn
1362-3095journal_volume
87pub_type
杂志文章abstract：PURPOSE:To determine whether murine lymphoid cell lines can discriminate between high- and low-LET (linear energy transfer) radiation-induced DNA lesions. MATERIALS AND METHODS:Sensitivity to killing by DNA-incorporated 3H and 125I decays, accumulated during storage in the gas phase of a liquid nitrogen tank, was dete...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110120337
更新日期：2002-06-01 00:00:00
abstract：PURPOSE:To investigate the induction and persistence of chromosome aberrations in mouse bone marrow cells after X-ray exposure and to detect differential involvement of individual chromosomes in translocations. MATERIALS AND METHODS:Male and female Swiss mice were exposed to 1 and 3 Gy of X-rays. Chromosome aberration...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139593
更新日期：1999-09-01 00:00:00
abstract：PURPOSE:To investigate the role of tumour versus non-tumour factors in the specific response to serotonin (5-HT) of tumour-feeding arterioles (TFA). MATERIALS AND METHODS:Using mouse models of intra-vital microscopy, the response to topical administration of 5-HT was studied in arterioles feeding tumours: fibrosarcoma...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141519
更新日期：1998-09-01 00:00:00
abstract：:The studies of the late effects of atomic-bomb (A-bomb) radiation on the immune system were started about 20 years after the bombings in 1945. The most remarkable late effects of radiation are the functional and quantitative abnormalities of T and B cells in survivors exposed to high doses (> or = 1.0 Gy). Abnormaliti...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009514551491
更新日期：1995-11-01 00:00:00
abstract：PURPOSE:The nature of the transferrable factor which goes from irradiated objects to bystander objects remains undefined. Most agree that a chemical entity is the likely 'factor' although some authors have produced in vitro evidence for the involvement of a physical component or a very potent volatile capable of travel...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.698366
更新日期：2012-08-01 00:00:00
abstract：PURPOSE:To investigate whether an adaptive response can be induced in mice which were pre-exposed to 900 MHz radiofrequency fields. MATERIALS AND METHODS:Adult male Kunming mice were exposed to 900 MHz radiofrequency fields (RF) at power intensities of 12, 120 and 1200 μW/cm(2) for 1 h/day for 14 days and then subject...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.550981
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:In order to understand the molecular mechanism of nucleobase damage caused by ultrasoft X-ray irradiation, guanine radicals have been studied using an X-band EPR (electron paramagnetic resonance) spectrometer installed in a synchrotron soft X-ray beamline. MATERIALS AND METHODS:Guanine pellets were irradiated ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000400017689
更新日期：2004-11-01 00:00:00
abstract：:Purpose: To summarize existing knowledge and to understand individual response to radiation exposure, the MELODI Association together with CONCERT European Joint Programme has organized a workshop in March 2018 on radiation sensitivity and susceptibility.Methods: The workshop reviewed the current evidence on this matt...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2019.1665209
更新日期：2020-03-01 00:00:00
abstract：:We characterized in vitro the effects of gamma-radiation (12.5-100 cGy) on pulmonary microvascular endothelial cell (PMEC) morphology and F-actin organization. Cellular retraction was documented by phase-contrast microscopy and the organization of actin microfilaments was determined by immunofluorescence. Characteriza...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551471
更新日期：1993-09-01 00:00:00
abstract：:Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1589013
更新日期：2019-10-01 00:00:00
abstract：:Ionizing radiation induces both quantitative and qualitative changes in the lymphoid cells of both man and experimental animals, including inhibition of antibody responses. However, the cellular basis of this immunological lesion is not clear. In the present study, groups of mice were exposed to 2.0 Gy gamma-rays or s...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550531
更新日期：1995-04-01 00:00:00
abstract：:Studies of early physical interactions of ionizing radiation in biological medium have evolved from water cylinders or spheres to structured volumes representing nucleosomal DNA, based on spatial co-ordinates for each individual atom. Regarding the physico-chemical and chemical stages, the models of DNA have evolved f...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551441
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530000138844
更新日期：2000-02-01 00:00:00
abstract：PURPOSE:To investigate the mechanisms inducing and maintaining the permanent elimination of low dose hyper-radiosensitivity (HRS) in cells given a dose of 0.3 Gy at low dose-rate (LDR) (0.3 Gy/h). MATERIALS AND METHODS:Two human HRS-positive cell lines (T-47D, T98G) were used. The effects of pretreatments with transfo...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2014.906767
更新日期：2014-07-01 00:00:00
abstract：PURPOSE:Exposure to ionizing radiation induces plant defenses by regulating the expression of response genes. The systemic acquired resistance deficient 1 (SARD1) is a key gene in plant defense response. In this study, the function of Oryza sativa SARD1 (OsSARD1) was investigated after exposure of seeds/plants to ioniz...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2017.1297901
更新日期：2017-07-01 00:00:00
abstract：PURPOSE:Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. MATERIALS AND METHODS:Paddy field microcosm samples were continuously exposed at a dose rate o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.549534
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.809170
更新日期：2013-11-01 00:00:00
abstract：:Terminally differentiated cells usually do not divide and are, thus, reproductively dead. To elucidate the significance of radiation-enhanced differentiation to reproductive cell death, murine erythroid progenitor cells were gamma-irradiated in plasma clot cultures and the development of haemoglobinized clones was stu...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145869
更新日期：1996-03-01 00:00:00
abstract：:Recent evidence indicates that isoeffect doses for spinal cord and kidney may be overestimated for fraction sizes as small as 1 or 2 Gy when calculated from a linear-quadratic (LQ) model fitted to data obtained for fraction sizes larger than 2 Gy. Reasons for this are unknown, but possible interpretations include exha...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814551461
更新日期：1988-07-01 00:00:00
abstract：:An assay system was developed to detect changes of restriction fragment profiles obtained after pulsed-field gel electrophoresis (PFGE) for DNA from mammalian cells that were irradiated and incubated for repair. DNA was prepared from irradiated log-phase human melanoma cells (MRI 221) after incubation for repair (6 h)...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144518
更新日期：1996-12-01 00:00:00
abstract：:It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009114551821
更新日期：1991-07-01 00:00:00
abstract：:Most estimates of the radiation dose lethal to 50 per cent of a human population are based on historical data taken from well-known experiences reflecting inadequately known physical and biological conditions, or from medical procedures where individual patients received advantages of modern clinical care. It has been...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550481
更新日期：1989-03-01 00:00:00
abstract：:This study evaluated the cell-mediated (CMI) and humoral immune responses in four Beagle dogs five to six years after single inhalation exposures to different monodisperse 239PuO2 aerosols (0.72-1.4 microns activity median aerodynamic diameter). These exposures resulted in initial lung burdens ranging from 19 to 35 kB...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550311
更新日期：1989-02-01 00:00:00
abstract：PURPOSE:To determine the relationships between the frequencies of radiation-induced chromosomal alterations and the extent of apoptosis in G0 human lymphocytes. MATERIAL AND METHODS:G0 human peripheral blood lymphocytes (HPBL) were X or gamma-irradiated, in the presence or absence of the repair inhibitor cytosine arab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500303690
更新日期：2005-08-01 00:00:00
abstract：PURPOSE:To assess the possibility that extremely low frequency (ELF) magnetic fields obstruct the damage repair process, the gene conversion frequency and cell cycle kinetics in a DNA repair-proficient and nucleotide excision repair (NER)-deficient strain of diploid yeast Saccharomyces cerevisiae. MATERIALS AND METHOD...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000310001621437
更新日期：2003-11-01 00:00:00
abstract：:Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately t...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553008914551971
更新日期：1989-11-01 00:00:00
abstract：PURPOSE:To investigate and compare the propensity of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL) obtained from unaffected healthy individuals and ataxia telangectasia (A-T) patients to undergo apoptosis after X-ray exposure. MATERIAL AND METHODS:The LCL were exposed to 1-4 Gy X-rays at a dose-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140041
更新日期：1999-06-01 00:00:00
abstract：PURPOSE:To evaluate the radiomodulatory influence of a leaf extract of Mentha piperita (Linn) on hepatic antioxidant status and lipid peroxidation in Swiss albino mice. MATERIALS AND METHODS:Animals were given either double distilled water or leaf extract of M. piperita orally (1 g/kg bwt/day) once a day for three con...
journal_title：International journal of radiation biology
pub_type： 杂志文章,收录出版
doi：10.1080/09553000600771523
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:Despite proven antitumor activity of gemcitabine in chemoradiotherapy of advanced head and neck cancer, many authors refer to severe acute and late local and haematological toxicity. Fludarabine does imply nearly the same mechanisms of action as gemcitabine, inhibiting various enzymes involved in DNA replicatio...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802241754
更新日期：2008-08-01 00:00:00
abstract：PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2016.1206234
更新日期：2016-09-01 00:00:00
