Abstract：
:Purpose Radiolabeled antibodies and peptides hold promise for molecular radiotherapy but are often limited by a low payload resulting in inadequate delivery of radioactivity to tumour tissue and, therefore, modest therapeutic effect. We developed a facile synthetic method of radiolabeling indium-111 (111In) to epidermal growth factor (EGF)-gold nanoparticles (111In-EGF-Au NP) with a high payload. Materials and methods EGF-Au NP were prepared via an interaction between gold and the disulphide bonds of EGF and radiolabeled using 111InCl3. Targeting efficiency was investigated by quantitating internalized radioactivity and by confocal imaging following exposure of MDA-MB-468 (1.3 × 106 EGFR/cell) and MCF-7 (104 EGFR/cell) cells to Cy3-EGF-Au NP. Cytotoxicity was evaluated in clonogenic assays. Results The proportion of total administered radioactivity that was internalized by MDA-MB-468 and MCF-7 cells was 15% and 1.3%, respectively (mixing ratio of EGF:Au of 160). This differential uptake in the two cell lines was confirmed using confocal microscopy. 111In-EGF-Au NP were significantly more radiotoxic to MDA-MB-468 than MCF-7 cells with a surviving fraction of 17.1 ± 4.4% versus 89.8 ± 1.4% (p < 0.001) after exposure for 4 h. Conclusions An 111In-labeled EGF-Au nanosystem was developed. It enabled targeted delivery of a high 111In payload specifically to EGFR-positive cancer cells leading to radiotoxicity that can be exploited for molecularly targeted radiotherapy.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Song L,Falzone N,Vallis KAdoi
10.3109/09553002.2016.1145360subject
Has Abstractpub_date
2016-11-01 00:00:00pages
716-723issue
11eissn
0955-3002issn
1362-3095journal_volume
92pub_type
杂志文章abstract：PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000110075220
更新日期：2001-12-01 00:00:00
abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000801998875
更新日期：2008-05-01 00:00:00
abstract：PURPOSE:To calculate survival of human haemopoietic stem cells irradiated by alpha particles from 149Tb (initial energy 3.97 MeV) and 211At (average initial energy 6.87 MeV). METHODS:Following as closely as possible radiobiological data, Monte Carlo methods were used to calculate passages of alpha particles (originati...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141771
更新日期：1998-07-01 00:00:00
abstract：PURPOSE:To reduce retention and toxicity of the alpha particle emitter polonium-210 in rats by newly developed chelating agents. MATERIALS AND METHODS:Repeated subcutaneous chelation was conducted after intravenous injection of 210Po nitrate. For reduction of 210Po retention the treatment with vicinal dithiols meso-an...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050151691
更新日期：2000-10-01 00:00:00
abstract：PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099140474
更新日期：1999-03-01 00:00:00
abstract：PURPOSE:To calculate the depth distribution of radioactive decays in bone by fitting a calculated alpha-particle energy spectrum to a measured spectrum. The dose to bone surface cells is then calculated from the depth distribution. MATERIALS AND METHODS:The spectra are calculated using Monte Carlo methods and a numeri...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098142734
更新日期：1998-01-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514551051
更新日期：1995-08-01 00:00:00
abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009414551661
更新日期：1994-11-01 00:00:00
abstract：PURPOSE:To investigate the ability of electromagnetic (EM) field pre-exposures to induce protection in chick embryos against subsequent ultraviolet (UV) light exposure. MATERIALS AND METHOD:Chick embryos in the 4th day of gestation were exposed for 20 minutes (short term) or 96 hours (long term) to 60 Hz, 8 microT mag...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139142
更新日期：1999-12-01 00:00:00
abstract：PURPOSE:To evaluate a transcriptomic approach to identify healthy women at increased risk of breast cancer due to G2-radiosensitivity and look at transcripts that are differentially expressed between individuals. MATERIALS AND METHODS:We perform the first study to assess the association of G2 radiosensitivity with bas...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701317366
更新日期：2007-06-01 00:00:00
abstract：:Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145634
更新日期：1996-05-01 00:00:00
abstract：:In mice, external X- or gamma-irradiation may induce thymic lymphomas or myeloid leukaemias, while bone-seeking alpha-emitters may induce osteosarcomas and, to a lesser extent, acute myeloid leukaemia. The present paper aims to review briefly some of the experimental data with respect to the molecular mechanisms under...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553009014550851
更新日期：1990-04-01 00:00:00
abstract：:Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008914550651
更新日期：1989-04-01 00:00:00
abstract：:PACE (programmable, autonomously controlled electrodes) pulsed field gel electrophoresis technology was used to develop a DNA double-strand break assay that simultaneously combined (1) resolution across a broad range of DNA sizes, (2) high sensitivity (in terms of detecting dsb at low doses) and (3) speed. A 48 h PACE...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314550021
更新日期：1993-01-01 00:00:00
abstract：PURPOSE:To examine whether the levels of micronuclei induction, as a marker for genomic instability in the progeny of X-irradiated cells, correlates with DNA repair function. MATERIALS AND METHODS:Two repair deficient cell lines (X-ray repair cross-complementing 1 [XRCC1] deficient cell line [EM9] and X-ray repair cro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.752596
更新日期：2013-04-01 00:00:00
abstract：PURPOSE:To evaluate the radiomodulatory influence of a leaf extract of Mentha piperita (Linn) on hepatic antioxidant status and lipid peroxidation in Swiss albino mice. MATERIALS AND METHODS:Animals were given either double distilled water or leaf extract of M. piperita orally (1 g/kg bwt/day) once a day for three con...
journal_title：International journal of radiation biology
pub_type： 杂志文章,收录出版
doi：10.1080/09553000600771523
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:The exploration of immune mechanisms of the tumour-inhibitory effect of exposures to low-level fractions of X-rays. MATERIALS AND METHODS:BALB/c mice were exposed to whole-body daily irradiations with 0.01, 0.02, or 0.1 Gy X-rays per day for 5 days/week for two weeks. Then, mice were intravenously injected wit...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.519422
更新日期：2011-02-01 00:00:00
abstract：:Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0.1...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009514550791
更新日期：1995-06-01 00:00:00
abstract：:The response to radiation of seven osteosarcoma cell lines was analysed by in vitro colony-forming assay and compared with that of eight human fibroblast strains. The values of D0, the surviving fraction after 2 Gy (S2Gy), and the mean inactivation dose (D) of osteosarcoma cells in log-phase culture were significantly...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214552351
更新日期：1992-10-01 00:00:00
abstract：:Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097144265
更新日期：1997-02-01 00:00:00
abstract：PURPOSE:Public concern about radiation protection of the environment and non-human species is increasing. The potential impact of chronic gamma radiation on the bacterial community in a rice paddy ecosystem was investigated. MATERIALS AND METHODS:Paddy field microcosm samples were continuously exposed at a dose rate o...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2010.549534
更新日期：2011-07-01 00:00:00
abstract：PURPOSE:As the most recent epidemiological studies provide no definite conclusions about the effects of 50/60 Hz magnetic fields (MFs) on the incidence of leukaemia in humans, animal models in a well-controlled environment are useful for evaluating the possibility of an association between MFs and leukaemia. The presen...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000050029011
更新日期：2000-06-01 00:00:00
abstract：:During previous studies on the relationship between thermosensitivity and intracellular pH (pHi) regulation in mammalian cells, we observed that the thermoresistant TR-4 cells appeared to be resistant to manipulations of the Na+/H+ antiport, one of the two primary membrane regulators of pHi. We hypothesized that this ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144969
更新日期：1996-10-01 00:00:00
abstract：:CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551361
更新日期：1993-08-01 00:00:00
abstract：:Context: Some individuals are reporting being sensitive to electromagnetic fields. They report some unspecific symptoms like headeache, sleep disruption, heart palpitations, itching, etc. The lack of scientific objective links between EHS symptoms and EMF exposure renders the diagnosis of EHS more complex. Purpose: Ou...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1569776
更新日期：2019-06-01 00:00:00
abstract：:Radiation-induced DNA double-strand breaks (dsb) were studied in Ehrlich ascites tumour cells (EATC) by sedimentation in neutral sucrose gradients at low centrifuge speed. Dsb induction was found to be linear with dose with a frequency of: ndsbmr-1D-1 = (11.7 +/- 2) x 10(-12)Gy-1 for 140 kV X-rays and ndsbmr-1D-1 = (1...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553008814552201
更新日期：1988-11-01 00:00:00
abstract：:Induction by gamma-radiation, UV radiation or hydroxyurea of RecA gene product synthesis in Escherichia coli, monitored as beta-D-galactosidase in recA-lacZ fusion strains, was shown to be inhibited if 2-mercaptoethylamine (MEA) was added before treatment with the inducing agents. If cysteine (Cys) at low concentratio...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096146228
更新日期：1996-01-01 00:00:00
abstract：:The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551951
更新日期：1990-10-01 00:00:00
abstract：PURPOSE:The prolonged delivery times associated with intensity modulated radiation therapy (IMRT) may reduce treatment effectiveness of radiation therapy for cancers with short repair half-times. In this study, in vitro radiation experiments with DU-145 prostate cancer cells were designed to quantify the half-time of s...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000802061285
更新日期：2008-06-01 00:00:00
abstract：:Purpose: Cancer is one of the most common causes of mortality and morbidity worldwide. Vaccines have been emerged as an attractive approach for their capacity of eliciting long-term immune response targeting cancer cells. Attenuated avirulent Toxoplasma gondii stimulate immunity and activate antitumor cells thereby el...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1739772
更新日期：2020-06-01 00:00:00
