Abstract:
PURPOSE:To study the relationship between cell reproductive death and exchange frequency in SW-1573 human lung tumour cells with and without incorporated iodo-deoxyuridine (IdUrd) following irradiation of plateau-phase cultures with y-rays. METHOD:Linear-quadratic (LQ) analysis was performed for the data on clonogenic survival and on the frequency of chromosomal exchanges studied with fluorescence in situ hybridization in chromosomes X and 2. RESULTS:Differences in the LQ parameters alpha and beta of both non-sensitized and sensitized chromosomes were found. In both chromosomes an increase in the number of chromosomal exchanges in IdUrd-radiosensitized cells compared with non-sensitized cells was observed. The alpha-enhancement factors of 1.7 and 1.9 for the X-chromosome and for chromosome 2, respectively, are similar. For the X-chromosome, the beta coefficient increased by a factor of 3.9 and for chromosome 2 by a factor of 1.4. After correction to a full genome equivalence, no significant difference in alpha was found between chromosomes X and 2 for both control and sensitized cells. In contrast, an almost 2.8 times higher beta was found for the sensitized X-chromosome compared to this value for chromosome 2. CONCLUSIONS:It can be concluded that the linear-quadratic analysis of dose-response relationships offers insights into the correlation between cell survival and induction of exchanges in non-sensitized and radiosensitized cells.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Franken NA,Ruurs P,Ludwików G,van Bree C,Kipp JB,Darroudi F,Barendsen GWdoi
10.1080/095530099140474subject
Has Abstractpub_date
1999-03-01 00:00:00pages
293-9issue
3eissn
0955-3002issn
1362-3095journal_volume
75pub_type
杂志文章abstract::The mechanisms of radiation damage production are used to examine the following premises: (1) the number of DNA double-strand breaks per unit dose increases with dose; (2) cell type to cell type variations in yield of DNA dsb per dose occur. Two stages of damage production are identified as possible sources of damage ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014551251
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
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pub_type: 杂志文章,评审
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doi:10.3109/09553002.2011.595874
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.3109/09553001003789596
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:2020-12-03 00:00:00
abstract:PURPOSE:The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene regulation occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role at the post-transcriptional gene regulation. miRNA a...
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journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/095530099139845
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:γ-rays (IR) cause an increase in intracellular calcium [Ca(2+)], alters contractility and triggers apoptosis via the activation of protein kinase C in intestinal guinea pig smooth muscle cells. The present study investigated the role of the mitochondria in these processes and characterized proteins involved in ...
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doi:10.3109/09553002.2014.911988
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journal_title:International journal of radiation biology
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doi:10.1080/09553009314450831
更新日期:1993-05-01 00:00:00
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doi:
更新日期:2009-01-01 00:00:00
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journal_title:International journal of radiation biology
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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