Abstract:
PURPOSE:To evaluate and optimize the radioprotective ability of the most potent fraction of an aqueous extract of Coronopus didymus in whole body gamma-irradiated Swiss albino mice and to evaluate the antioxidant status and lipid peroxidation of the livers of the surviving mice. To correlate the free radical scavenging studies with in vivo radioprotection ability. MATERIALS AND METHODS:Swiss albino mice were treated with either vehicle or the different doses of extract/fraction suspension by an i.p. route, 30 min before exposure to 10 Gy gamma-irradiation and the animals were monitored twice daily for any signs of radiation toxicity and mortality. Radiation dose response (7-11 Gy), optimization of route, time of drug administration and evaluation of dose response factor (DRF) at the best dose of the fraction was studied. Endogenous antioxidant status and lipid peroxidation of the livers of the mice surviving on the 31st day was evaluated by using spectrophotometric methods. RESULTS:The most active free radical scavenging fraction (CDF1) as assessed by competition kinetic studies using pulse radiolysis showed maximum in vivo radioprotection of 70% at a dose of 400 mg/kg body weight (bw) compared to corresponding 10 Gy irradiated control. Optimum radioprotection was observed upon i.p. administration, 30 min prior to 10 Gy irradiation and DRF at a dose of 400 mg/kg bw for 30 day survival was found to be 1.07. The levels of endogenous antioxidant enzymes and lipid peroxidation in the CDF1 treated surviving mice were found to reverse back to their normal levels. CONCLUSIONS:The optimum dose, time and route of drug administration for maximum radioprotection by CDF1 were determined. The reversal of the levels of endogenous antioxidant enzymes and lipid peroxidation indicates reduced oxidative stress in CDF1 treated surviving mice.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Prabhakar KR,Veerapur VP,Parihar KV,Priyadarsini KI,Rao BS,Unnikrishnan MKdoi
10.1080/09553000600876686subject
Has Abstractpub_date
2006-08-01 00:00:00pages
525-36issue
8eissn
0955-3002issn
1362-3095pii
T67655UV41238403journal_volume
82pub_type
杂志文章abstract::The bacterial enzyme PvuII, which generates blunt-ended DNA double-strand breaks, and T4 DNA ligase, which seals adjacent DNA fragments in coupling to ATP cleavage, were introduced in mouse C3H10T1/2 fibroblasts using osmolytic shock of pinocytic vesicles. Cells were then assayed for their clonogenic ability. In agree...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550851
更新日期:1991-04-01 00:00:00
abstract::The past recommendations of the ICRP about dose limits to the skin are reviewed. Recently, an ICRP Task Group has been revisiting the old arguments and setting them against new data. With the exception of the function of cells in the skin associated with immunocompetence, non-stochastic effects have been well characte...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009014550961
更新日期:1990-04-01 00:00:00
abstract::Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551111
更新日期:1990-05-01 00:00:00
abstract::The relationship between cellular radiosensitivity and normal-tissue response to radiotherapy in individual cancer patients has attracted increasing attention over the last few years. Recent work has suggested that a correlation exists between fibroblast sensitivity and normal-tissue reactions. We have examined the ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145021
更新日期:1996-09-01 00:00:00
abstract:PURPOSE:To examine how folate status influences chromosomal damage following X-ray irradiation. MATERIAL AND METHODS:In an animal study, mice were fed either a low, basal, or high folic acid diet (0, 2, or 40 mg/kg diet, respectively) for 4 weeks, and then given total body irradiation (TBI) at 0.5 Gy. In a human study...
journal_title:International journal of radiation biology
pub_type: 杂志文章,随机对照试验
doi:10.1080/09553000600721817
更新日期:2006-04-01 00:00:00
abstract::Objective: To quantify DNA damage in patients undergoing non-contrast and contrast-enhanced 18F-FDG PET/CT whole body positron emission tomography/computed tomography (WB PET/CT) investigations using comet assay technique and micronucleus assay, and to study the effect of other baseline parameters of patients on DNA d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1577569
更新日期:2019-06-01 00:00:00
abstract::The aim was to review and summarize the results of 40 years of study concerning the response to ionizing radiation of the pair of L5178Y (LY) sublines, LY-R and LY-S, that differ in sensitivity to various DNA-damaging agents, among them X- and gamma-rays. The reviewed data indicate the key importance of DNA damage rep...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000500143534
更新日期:2005-05-01 00:00:00
abstract::Pulsed-field electrophoresis is being used extensively in the gene mapping studies and in the analysis of DNA strand breakage by ionizing radiation. We have evaluated the relationship between the fraction of S phase DNA in a cell population and its ability to modify the migration of DNA in pulsed-field gel electrophor...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145427
更新日期:1996-06-01 00:00:00
abstract::Purpose: Present study deals with the role of gamma irradiation in modulating lead (Pb) tolerance of Aspergillus niger van Tieghem. and Penicillium cyclopium Westling. Materials and methods: After being exposed to gamma absorbed doses those fungal strains were subjected to heavy metal uptake efficacies and anti-oxidat...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1569769
更新日期:2019-06-01 00:00:00
abstract::Existing models of chromosome aberrations produced by ionizing-radiation predict equal numbers of dicentrics and translocations if the dose is so low that complex aberrations can be ignored. We show that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at highe...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145977
更新日期:1996-02-01 00:00:00
abstract:PURPOSE:Since the number of cancer patients treated by proton irradiation has increased in the last few years, it seems appropriate to study dose-dependent effects of proton irradiation on mammalian tissues in more detail. MATERIALS AND METHODS:Tissue samples of normal skin of mouse and swine, of a human tumour model ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000412331283515
更新日期:2004-08-01 00:00:00
abstract::Resolving the molecular mechanisms of radiation oncogenesis represents an important but daunting challenge in radiation research. This brief review outlines the principal oncogenic mechanisms that need to be considered in the context of radiation effects on the genome, how these might relate to specific gene and chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414550081
更新日期:1994-01-01 00:00:00
abstract::Purpose: Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1625462
更新日期:2019-09-01 00:00:00
abstract:PURPOSE:Despite proven antitumor activity of gemcitabine in chemoradiotherapy of advanced head and neck cancer, many authors refer to severe acute and late local and haematological toxicity. Fludarabine does imply nearly the same mechanisms of action as gemcitabine, inhibiting various enzymes involved in DNA replicatio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802241754
更新日期:2008-08-01 00:00:00
abstract::High doses of rHIL-1 protected C3H/HeN mice against lethal radiation damage. The optimum time of radioprotection was found when this cytokine was administered at 4 h and at 20 h before irradiation. The dose reduction factors when the IL-1 was administered at those times were about 1.30. The radioprotection at the opti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914551621
更新日期:1989-10-01 00:00:00
abstract:PURPOSE:Many strategies have been investigated to exclude the several side-effects of pharmacological or invasive treatments. Non-invasive pulsed magnetic field (PMF) treatment with no toxicity or side-effects can be an alternative to pharmacologic treatments. The purpose of this study was, therefore, to investigate th...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.835501
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:To evaluate how irradiation affects thyroid autoimmunity in mouse models of Hashimoto's thyroiditis and Graves' hyperthyroidism. MATERIALS AND METHODS:Non-obese diabetic (NOD)-H2(h4) mice spontaneously develop anti-thyroglobulin (Tg) antibodies and thyroiditis when supplied with sodium iodine (NaI) in the drin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802345910
更新日期:2008-09-01 00:00:00
abstract:PURPOSE:Therapy with low doses of ionising radiation (X-rays) exerts anti-inflammatory effects. Little is known about whether and how low doses of X-ray treatment modulate the inflammatory phenotype of macrophages, especially the secretion of Interleukin-1beta (IL-1β). MATERIALS AND METHODS:Macrophages were differenti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.689464
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:To investigate the relationship between the susceptibility of B-chronic lymphoid leukaemia (B-CLL) cells to DNA damage-induced apoptosis, the kinetics of DNA strand-break rejoining, and chromosome damage after exposure to ionizing irradiation. MATERIALS AND METHODS:Lymphocytes from B-CLL patients were gamma-ir...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110120364
更新日期:2002-08-01 00:00:00
abstract:PURPOSE:RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2016.1206233
更新日期:2017-01-01 00:00:00
abstract:PURPOSE:To compare the chromosomal radiosensitivity of C3H mouse acute myeloid leukaemia (AML) cell lines 7926 and 8709 and to investigate the mechanistic basis of the radiosensitivity observed in 7926. MATERIALS AND METHODS:Yields of chromosome aberrations following X-irradiation were determined in Giemsa-stained met...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110075220
更新日期:2001-12-01 00:00:00
abstract::Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514551051
更新日期:1995-08-01 00:00:00
abstract::Purpose: The key to increasing our understanding of low dose effects of ionizing radiation is cooperation and communication between research organizations around the world. The purpose of this article is to describe the Electric Power Research Institute (EPRI) program for such cooperation. Materials and methods: EPRI ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1569770
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:Immune cells accumulate in and around cancers and cooperate with each other using specific cytokines to attack the cancer cells. The heavy-ion beams for cancer therapy may stimulate immune cells and affect on the immune system. However, it is still poorly understood how the immune cells are stimulated by ion-be...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2012.636138
更新日期:2012-03-01 00:00:00
abstract::Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552351
更新日期:1991-09-01 00:00:00
abstract::It follows from the present work that the vascularization of the normal tissue present in the treatment volume limits the temperatures achieved during heat treatment of invasive ductal breast carcinoma. The temperatures can often be increased by giving fractionated heat treatment because heat treatment may reduce the ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114551821
更新日期:1991-07-01 00:00:00
abstract::Tikvah Alper's interest in science was broad, from scrapie to mammalian cells and cancer. Much of her own work focused on cell lethality, like that of many other radiobiologists, but this was natural because of the simplicity of the endpoint cell survival and its relevance to cancer therapy. Tikvah had broader interes...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143662
更新日期:1997-06-01 00:00:00
abstract:PURPOSE:To examine whether a simulated mobile telephone transmission at 915 MHz has an effect on cognitive function in man. MATERIALS AND METHODS:Thirty-six subjects in two groups were each given two training sessions and then three test sessions in a randomized three-way cross-over design. About 1 W mean power at 915...
journal_title:International journal of radiation biology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/095530099140375
更新日期:1999-04-01 00:00:00
abstract:PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.901579
更新日期:2014-06-01 00:00:00
abstract::The radiation chemistry of the oligomer d(TpApCpG) X-irradiated in aqueous solution containing glutathione was studied. Four products were isolated by HPLC and characterized by NMR spectroscopy. Two of the major products are isomers of a 5-hydroxy-5,6-dihydrothymine modification of d(TpApCpG). A dihydrothymine modific...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014552141
更新日期:1990-11-01 00:00:00