Abstract:
:Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Schmid E,Zitzelsberger H,Braselmann H,Gray JW,Bauchinger Mdoi
10.1080/09553009214552621subject
Has Abstractpub_date
1992-12-01 00:00:00pages
673-8issue
6eissn
0955-3002issn
1362-3095pii
7L7AL0XW2CL39TE8journal_volume
62pub_type
杂志文章abstract:PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.809170
更新日期:2013-11-01 00:00:00
abstract::We have re-examined the effect of detergent type, pH and temperature of lysis on the shape of the DNA elution dose response under non-denaturing conditions using plateau-phase CHO cells. Results practically identical to those previously published (Okayasu and Iliakis, 1989) were obtained, with a 1 h incubation at 60 d...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551211
更新日期:1992-04-01 00:00:00
abstract:PURPOSE:To understand the mechanisms of life-shortening due to early neoplastic death caused by chronic low dose-rate (LDR; 20 mGy/22 h/day) radiation which accumulates to a high dose (HD; 8 Gy) (LDR/HD) as reported previously. MATERIALS AND METHODS:Female B6C3F(1) mice were continuously exposed to LDR/HD gamma-rays u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.545861
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:It has been previously argued that the use of the one-electron oxidants (SCN)2(*-) and Br2(*-) with plasmid DNA leads to the formation of DNA guanyl radicals. These guanyl radical species are intermediates in the DNA damage produced by processes such as photo-ionization and ionizing irradiation. The present pap...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110069119
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518204
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:The sequelae of premature loss of ovarian function can undoubtedly have undesirable effects for a woman although radiotherapy is one of the most relevant treatment modalities for various types of malignancies. The aim of this study was to determine the effect of different doses of radiation on ovarian folliculo...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1864497
更新日期:2021-01-06 00:00:00
abstract:PURPOSE:To calculate the depth distribution of radioactive decays in bone by fitting a calculated alpha-particle energy spectrum to a measured spectrum. The dose to bone surface cells is then calculated from the depth distribution. MATERIALS AND METHODS:The spectra are calculated using Monte Carlo methods and a numeri...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142734
更新日期:1998-01-01 00:00:00
abstract::Mutations in the p53 tumour suppressor gene are found at high frequency in bladder cancer. There is strong evidence that p53 plays an important role in controlling the cell cycle after DNA damage by ionizing radiation. However, the effect of loss of p53 function on radiosensitivity is not yet clear. Radiotherapy combi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143491
更新日期:1997-07-01 00:00:00
abstract::We have examined the timing of gamma-irradiation-induced death in relation to cell cycle progression using a panel of mouse lymphoid or myeloid cell lines. Death was found to occur immediately after irradiation ('rapid interphase' death), or after arrest in G2 phase ('delayed interphase' death). In part II of this ser...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550261
更新日期:1994-02-01 00:00:00
abstract:PURPOSE:Ionizing radiation has been recognized to increase the risk of cardiovascular diseases (CVD). However, there is no consensus concerning the dose-risk relationship for low radiation doses and a mechanistic understanding of low dose effects is needed. MATERIAL AND METHODS:Previously, human umbilical vein endothe...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.905724
更新日期:2014-07-01 00:00:00
abstract::A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2018.1454616
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:We aimed for a quantitative evaluation that justifies guidelines for evacuation which take into consideration both the human and economic costs. To the best of our knowledge, such an evaluation has not been performed yet. The present guidelines published by the International Atomic Energy Agency (IAEA) are prob...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1779962
更新日期:2020-11-01 00:00:00
abstract:PURPOSE:Zearalenone is a potent estrogenic metabolite produced by some Fusarium and Gibberella species. Therefore, the aim of this study was to determine the accumulation of Technetium-[99m]-labeled zearalenone in mice organs as well as to study the protective effect of Lactobacillus plantarum. MATERIALS AND METHODS:T...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1524990
更新日期:2018-12-01 00:00:00
abstract::Radiation survival curves have been obtained for crypts in the small and large intestine of male BDF1 mice using the microcolony assay. Four regions of the small intestine and three regions of the large intestine were studied. One of the major objectives was to determine the optimal conditions for the microcolony assa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097144265
更新日期:1997-02-01 00:00:00
abstract::We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dode...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550371
更新日期:1994-03-01 00:00:00
abstract::One-cell mouse embryos were irradiated with X-rays or neutrons. Analysis of the first, second, and third postradiation mitoses revealed that the yields of structural aberrations increased linearly after exposure to both radiation qualities. For X-rays the aberration frequency decreased from the first to the third mito...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814551771
更新日期:1988-09-01 00:00:00
abstract:PURPOSE:To examine the heat sensitivity of DNA-dependent protein kinase (DNA-PK) activity in a variety of cultured mouse, hamster and human cell lines. MATERIALS AND METHODS:Eight cell lines, which have been routinely used in our laboratory, were examined. Cells were heated at 44.0 +/- 0.05 degrees C and DNA-PK activi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000310001596959
更新日期:2003-08-01 00:00:00
abstract:PURPOSE:Chironomus ramosus is one of the recently reported radiotolerant insects. Salivary gland cells of fourth instar larvae respond to ionizing radiations with increases in the levels of antioxidant enzymes and chaperone proteins. Here we made an attempt to study the state of nuclear DNA after exposure of larvae to ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1062572
更新日期:2015-01-01 00:00:00
abstract::Purpose: A study is presented of the irradiation of cancerous cervical cell line HeLa loaded with a platinum salt, betamethasone and deoxyglucose. The presence of the platinum increases the free-radical concentration and augments the cell death rate, whereas betamethasone or deoxyglucose induces radiosensitization by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1721594
更新日期:2020-05-01 00:00:00
abstract::Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2016.1137105
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:Theoretical modelling techniques are often used to simulate the action of ionizing radiations on cells at the nanometre level. Using monoenergetic vacuum-UV (VUV) radiation to irradiate DNA either dry or humidified, the action spectra for the induction of DNA damage by low energy photons and the role of water a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000050028913
更新日期:2000-06-01 00:00:00
abstract::Induction of mutations to 6-thioguanine resistance (TGr) by gamma-rays at three different dose-rates and molecular changes in the HPRT gene were studied in human lymphoblastoid WIL2-NS cells. Mutant induction showed a curvilinear dose-response for acute irradiation (30 Gy/h). The total mutant frequency was lower after...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145201
更新日期:1996-08-01 00:00:00
abstract::Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552351
更新日期:1991-09-01 00:00:00
abstract:PURPOSE:To examine whether a simulated mobile telephone transmission at 915 MHz has an effect on cognitive function in man. MATERIALS AND METHODS:Thirty-six subjects in two groups were each given two training sessions and then three test sessions in a randomized three-way cross-over design. About 1 W mean power at 915...
journal_title:International journal of radiation biology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/095530099140375
更新日期:1999-04-01 00:00:00
abstract:PURPOSE:To analyze the direct and transgenerational effects of exposure to low-dose 1 GHz (mobile phone/wireless telecommunication range) and 10 GHz (radar/satellite communication range) radiofrequency electromagnetic fields (RF-EMF) on the motility of ciliates Spirostomum ambiguum. MATERIALS AND METHODS:S. ambiguum w...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2014.868612
更新日期:2014-03-01 00:00:00
abstract::Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145634
更新日期:1996-05-01 00:00:00
abstract::The dose-rate effect has been the topic of extensive radiobiological studies and has important implications in radiation therapy and in the field of radiation protection. Three examples will be discussed: two in radiation therapy and one in protection. First, continuous low dose-rate interstitial brachytherapy may be ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009114550531
更新日期:1991-03-01 00:00:00
abstract::The relative contributions of the OH-mediated and direct radiation effect on the induction of DNA double-strand breaks (dsbs) were evaluated in two haploid yeast cell lines (GSH+ and gsh-) irradiated under oxic or hypoxic conditions in the absence or presence of 6 M glycerol as an OH radical scavenger. Gsh- cells are ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551721
更新日期:1993-11-01 00:00:00
abstract:PURPOSE:To give a state-of-the-art overview on the promise of three-dimensional (3-D) culture systems for anticancer drug development, with particular emphasis on multicellular tumor spheroids (MCTS). RESULTS AND CONCLUSIONS:Cell-based assays have become an integral component in many stages of routine anti-tumor drug ...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000701727531
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:The impact of the damage distribution to cellular survival and chromosomal aberrations following high Linear Energy Transfer (LET) radiation was investigated. MATERIALS AND METHODS:High LET iron-ions (500 MeV/n, LET 200 keV/μm) were delivered to G1-phase synchronized Chinese Hamster Ovary (CHO) cells located a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.767990
更新日期:2013-06-01 00:00:00