Abstract:
PURPOSE:To clarify the experimental conditions that might influence the release of cytokines in the culture medium and give some basic input for building a model for cytokine (e.g., Interleukin-6, IL-6) regulation in the case of 'sham irradiation' and after ionising radiation exposure. MATERIALS AND METHODS:The influence of cell type, cell density, medium volume, medium storage temperature and other methodological aspects on IL-6 and Interleukin-8 (IL-8) release were investigated. In addition, the effects over the time of different doses of gamma irradiation on the clonogenic survival of bystander cells and on the secretion of these cytokines were studied. RESULTS:We observed significant decreases of clonogenic survival in AG01522 and T98G cells after the transfer of medium collected 5 and 20 h after low doses of gamma irradiation. Concerning the Interleukins' measurements, our experiments showed that the aggregate removal modalities tested, and up to 10 freeze-thaw cycles, do not have significant influence on the measurements of IL-6 concentration in the medium. We also observed that the IL-6 accumulated in the medium of human fibroblasts is not degraded when maintained at 37 degrees C. Sets of experiments demonstrated that cell density or medium volume do not influence the release of IL-6. On the contrary, our results showed that IL-8 released by glioblastoma cells strongly depends on the amount of medium. Finally, the exposure of fibroblasts to gamma irradiation has influence on the release kinetics of both IL-6 and IL-8 with peculiar features. CONCLUSIONS:This study solved some of the methodological doubts concerning the study of bystander effects by means of the medium transfer technique; moreover it also highlighted some experimental aspects that need to be considered when approaching this sort of experiments.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Facoetti A,Mariotti L,Ballarini F,Bertolotti A,Nano R,Pasi F,Ranza E,Ottolenghi Adoi
10.1080/09553000903020016subject
Has Abstractpub_date
2009-08-01 00:00:00pages
690-9issue
8eissn
0955-3002issn
1362-3095pii
913451682journal_volume
85pub_type
杂志文章abstract:PURPOSE:Biomarkers of ionising radiation exposure are useful in a variety of scenarios, such as medical diagnostic imaging, occupational exposures, and spaceflight. This study investigates to what extent microRNA (miRNA) expression signatures in mouse peripheral blood can be used as biomarkers for exposures to radiatio...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.549537
更新日期:2011-07-01 00:00:00
abstract::We have measured clonal survival following gamma-irradiation of unstimulated (G0) T-lymphocytes from 35 donors, of 11 T-lymphocyte cell lines, of six lymphoblastoid cell lines, and of nine primary fibroblast strains for which we have G0 T-lymphocyte material from the same donor. Amongst the G0 lymphocytes we have resu...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552331
更新日期:1988-12-01 00:00:00
abstract:PURPOSE:To evaluate how irradiation affects thyroid autoimmunity in mouse models of Hashimoto's thyroiditis and Graves' hyperthyroidism. MATERIALS AND METHODS:Non-obese diabetic (NOD)-H2(h4) mice spontaneously develop anti-thyroglobulin (Tg) antibodies and thyroiditis when supplied with sodium iodine (NaI) in the drin...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802345910
更新日期:2008-09-01 00:00:00
abstract::Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550651
更新日期:1989-04-01 00:00:00
abstract:PURPOSE:The detection of long-term persistent chromosome aberrations in circulating haemopoietic stem cells after accidental radiation exposure. MATERIAL AND METHODS:Peripheral blood samples from highly exposed persons were collected 7-25 years after the radiation accidents in Moscow (1971), Kazan (1975) and Chernobyl...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139395
更新日期:1999-10-01 00:00:00
abstract::Human ovarian carcinoma parental and cisplatin-resistant cells were evaluated for their radiation sensitivity to high and low dose-rate irradiation and for the effectiveness of cisplatin in radiosensitization. The cisplatin resistant variant A2780cp showed increased radiation resistance for both low dose-rate (LDRI) a...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145634
更新日期:1996-05-01 00:00:00
abstract::This study evaluated the cell-mediated (CMI) and humoral immune responses in four Beagle dogs five to six years after single inhalation exposures to different monodisperse 239PuO2 aerosols (0.72-1.4 microns activity median aerodynamic diameter). These exposures resulted in initial lung burdens ranging from 19 to 35 kB...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550311
更新日期:1989-02-01 00:00:00
abstract:PURPOSE:To investigate whether pre- and post-drug magnetic field (MF) exposure of 50 Hz, 0.2 mT has any significant effect on pentylenetetrazol (PTZ)-induced seizures in mice. MATERIAL AND METHODS:MF was generated by a pair of Helmholtz coils. Seizures were induced by PTZ injection intraperitoneally (i.p.) at a dose o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701206676
更新日期:2007-04-01 00:00:00
abstract:PURPOSE:To study the influence of TGF-beta1 and TGF-beta-neutralizing antibodies on the clonogenic activity and terminal differentiation of rat lung fibroblasts following radiation exposure. MATERIAL AND METHODS:Early passage rat lung fibroblasts were used in this study. Colony formation assays were applied to determi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138501
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:To examine the protective effects of histamine on intestinal damage produced by gamma-radiation. MATERIALS AND METHODS:56 mice were divided into 4 groups. Histamine and Histamine-10 Gy groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 20 hours before irradiation and continued until ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701570238
更新日期:2007-10-01 00:00:00
abstract::CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551361
更新日期:1993-08-01 00:00:00
abstract::The aim was to investigate further the relationship between radiation-induced mitotic delay and the expression of chromosome damage in V79 cells. Recently published data on the time-course of chromosome aberrations in V79 first-cycle metaphases after exposure to 10.4 MeV u(-1) Ar ions (LET = 1226 keV microm(-1)) were ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000400027902
更新日期:2005-01-01 00:00:00
abstract:PURPOSE:Exposure to ionizing radiation induces plant defenses by regulating the expression of response genes. The systemic acquired resistance deficient 1 (SARD1) is a key gene in plant defense response. In this study, the function of Oryza sativa SARD1 (OsSARD1) was investigated after exposure of seeds/plants to ioniz...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1297901
更新日期:2017-07-01 00:00:00
abstract:PURPOSE:The aim of this research was to demonstrate the effects of 50 Hz sinusoidal extremely low frequency of magnetic fields (ELF-MF) on preincubated chicken embryo hearts at histopathological, biochemical, and ultrastructural levels. MATERIALS AND METHODS:Ninety healthy fertilized eggs were divided into six groups ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.754556
更新日期:2013-04-01 00:00:00
abstract::In a series of studies to investigate activation energies and thermal enhancements of various chemotherapeutic agents the effect of 5-fluoruracil (5FU), an antimetabolite, on murine tumour cells was studied at elevated temperatures. Animal tumours were early generation isotransplants of a spontaneous fibrosarcoma, FSa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550211
更新日期:1991-01-01 00:00:00
abstract:PURPOSE:To investigate the consequence of continuous low dose-rate exposure to gamma-rays on ornithine decarboxylase (ODC EC 4.1.1.17) activity in organs of rat. MATERIALS AND METHODS:Young male Wistar rats were irradiated at 1.1, 2.1 and 12.9 cGy/day in the dose ranges of 9-165, 17-315 and 100-2000 cGy, respectively,...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099140645
更新日期:1999-02-01 00:00:00
abstract::Resolving the molecular mechanisms of radiation oncogenesis represents an important but daunting challenge in radiation research. This brief review outlines the principal oncogenic mechanisms that need to be considered in the context of radiation effects on the genome, how these might relate to specific gene and chrom...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553009414550081
更新日期:1994-01-01 00:00:00
abstract::Male SAS/4 mice were injected i.v. with 6.6 kBq 239Pu-citrate. After 1 or 24 h a single i.p. injection of 15 or 30 mumol kg-1 or repeated (three or four) daily injections of 30 mumol kg-1 of tetra-THB-spermine were given, and at 4 or 7 days Pu retention was measured in liver, kidneys and femur. Besides tetra-THB-sperm...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550141
更新日期:1989-01-01 00:00:00
abstract:PURPOSE:Human diseases associated with acute radiation responses are rare genetic disorders with common clinical and biological features including radiosensitivity, genomic instability, chromosomal aberrations, and frequently immunodeficiency. To determine what molecular assays are predictive of cellular radiosensitivi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701797039
更新日期:2008-02-01 00:00:00
abstract:PURPOSE:To determine the contribution of intra-alveolar cells as opposed to cells fixed in the interstitium in the development of radiation-induced lung injury. MATERIALS AND METHODS:C3H/HeN mice were irradiated to the thorax with various doses of radiation. The cellular composition and cytokine production were assess...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000076894
更新日期:2003-03-01 00:00:00
abstract:PURPOSE:To study the natural radionuclides in the freshwater fish samples around the uranium mining areas of Bagjata and Banduhurang, East Singhbhum, Jharkhand, India. MATERIALS AND METHODS:The naturally occurring radioisotopes of uranium, U(nat), consisting of (234)U, (235)U and (238)U; (226)Ra, (230)Th and (210)Po w...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.492490
更新日期:2010-11-01 00:00:00
abstract::237Np nitrate was injected intravenously into 4-week-old (young) and 10-12-week-old (adult) female albino Sprague-Dawley rats. The amounts given were 52 (young), 5.2 (adult) and 26 kBq kg-1 body weight (adult). The microscopic distribution in the femur and the lumbar vertebrae was studied. Initially, neptunium was dis...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550511
更新日期:1993-03-01 00:00:00
abstract:PURPOSE:The space environment contains two major biologically significant influences; space radiations and microgravity. The 53 kDa tumour suppressor protein (p53) plays a role as a guardian of the genome through the activity of p53-centered signal transduction pathways. The aim of this study was to clarify the biologi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003789596
更新日期:2010-08-01 00:00:00
abstract::PACE (programmable, autonomously controlled electrodes) pulsed field gel electrophoresis technology was used to develop a DNA double-strand break assay that simultaneously combined (1) resolution across a broad range of DNA sizes, (2) high sensitivity (in terms of detecting dsb at low doses) and (3) speed. A 48 h PACE...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550021
更新日期:1993-01-01 00:00:00
abstract::Two novel nitroimidazole-carboranes were examined for their uptake and retention in two experimental murine solid tumours and in some normal tissues, using in vivo 11B magnetic resonance spectroscopy. The compounds were injected i.p. at 0.8mmol/kg into mice bearing either the SCCVII/Ha squamous cell carcinoma or KHT s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144798
更新日期:1996-11-01 00:00:00
abstract::Purpose: Dose assessment plays an important role in case of radiological accidents and can be performed by scoring structural changes of chromosome morphology induced in cells by ionizing radiation. The results of such a test are biased by scorer experience, therefore, simple to learn assays are recommended to be used...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1625462
更新日期:2019-09-01 00:00:00
abstract::Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, a multiscale high-throughput multi-omics analysis was applied with a protocol for chronic exposure to the rat kidney. Methods: Male and female rats were contaminated for nine months through their drinking water with a nont...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1577567
更新日期:2019-06-01 00:00:00
abstract:PURPOSE:To assess changes in mitochondrial morphology and mitophagy induced by simulated sunlight irradiation (SSI) and how these changes are modulated by mitochondrial activity and energy source. MATERIALS AND METHODS:Human malignant amelanotic melanoma A375 cells were pre-treated with either a mitochondrial activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.556175
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1101500
更新日期:2016-01-01 00:00:00
abstract:PURPOSE:Radioresistance is highly correlated with radiotherapy failure in clinical cancer treatment. In the current study, we sought to examine the efficacy of Celecoxib and Afatinib co-treatment as radiosensitizers in the management of non-small cell lung cancer (NSCLC) A549 cells. MATERIALS AND METHODS:Generally, A5...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1846817
更新日期:2020-11-12 00:00:00