The effect of 5-fluorouracil at elevated temperatures on a spontaneous mouse tumour: Arrhenius analysis and tumour response.


:In a series of studies to investigate activation energies and thermal enhancements of various chemotherapeutic agents the effect of 5-fluoruracil (5FU), an antimetabolite, on murine tumour cells was studied at elevated temperatures. Animal tumours were early generation isotransplants of a spontaneous fibrosarcoma, FSa-II tumours, and C3Hf/Sed mice were used throughout. Cell survival curves for 5FU were obtained as a function of treatment time by in vitro treatment-in vivo lung colony assay at temperatures between 37.0 and 43.5 degrees C. The D0, or the treatment time to reduce surviving fraction from S to S/e in the exponential portion of the survival curve, decreased slightly from 37 degrees C treatment to 41.5 degrees C treatment. The D0 decreased substantially from 41.5 degrees C to 43.5 degrees C. Arrhenius-plot analysis indicated that the activation energies were 92.9 and 731 kJ/M at temperatures between 37 and 41.5 degrees C, and between 41.5 and 43.5 degrees C, respectively. The activation energy of 92.9 kJ/M for the temperature range from 37 to 41.5 degrees C was the lowest of the other agents, bleomycin, cis-diamminedichloroplatinum and 1,3-bis(2-chloroethyl)-N-nitrosourea, which have been investigated in our laboratory. This indicated that the thermal enhancement was smallest among these agents. In vivo experiments failed to demonstrate thermal enhancement of the anti-tumour effect of 5FU. Namely, combined 5FU and heat treatments at 41.5 and 43.5 degrees C did not prolong the tumour growth time compared with 5FU given at room temperature. No pH effect was found in an in vitro experiment and glucose administration did not enhance the anti-tumour effect of 5FU.


Int J Radiat Biol


Urano M,Kahn J,Reynolds R




Has Abstract


1991-01-01 00:00:00














  • The in vivo effects of low-intensity radiofrequency fields on the motor activity of protozoa.

    abstract:PURPOSE:To analyze the direct and transgenerational effects of exposure to low-dose 1 GHz (mobile phone/wireless telecommunication range) and 10 GHz (radar/satellite communication range) radiofrequency electromagnetic fields (RF-EMF) on the motility of ciliates Spirostomum ambiguum. MATERIALS AND METHODS:S. ambiguum w...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Sarapultseva EI,Igolkina JV,Tikhonov VN,Dubrova YE

    更新日期:2014-03-01 00:00:00

  • Rapid communication: effect of irradiation on lymphocyte proliferation and differentiation: potential of IL-6 in augmenting antibody responses in cultures of murine spleen cells.

    abstract::Ionizing radiation induces both quantitative and qualitative changes in the lymphoid cells of both man and experimental animals, including inhibition of antibody responses. However, the cellular basis of this immunological lesion is not clear. In the present study, groups of mice were exposed to 2.0 Gy gamma-rays or s...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Goud SN

    更新日期:1995-04-01 00:00:00

  • Molecular dissection of the response of the rice Systemic Acquired Resistance Deficient 1 (SARD1) gene to different types of ionizing radiation.

    abstract:PURPOSE:Exposure to ionizing radiation induces plant defenses by regulating the expression of response genes. The systemic acquired resistance deficient 1 (SARD1) is a key gene in plant defense response. In this study, the function of Oryza sativa SARD1 (OsSARD1) was investigated after exposure of seeds/plants to ioniz...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Jung IJ,Hwang JE,Han SM,Kim DS,Ahn JW,Choi HI,Kwon SJ,Kang SY,Kim JB

    更新日期:2017-07-01 00:00:00

  • Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans - joint RENEB and EURADOS inter-laboratory comparisons.

    abstract:PURPOSE:RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ainsbury E,Badie C,Barnard S,Manning G,Moquet J,Abend M,Antunes AC,Barrios L,Bassinet C,Beinke C,Bortolin E,Bossin L,Bricknell C,Brzoska K,Buraczewska I,Castaño CH,Čemusová Z,Christiansson M,Cordero SM,Cosler G,Mo

    更新日期:2017-01-01 00:00:00

  • The linear nonthreshold (LNT) model as used in radiation protection: an NCRP update.

    abstract:PURPOSE:The linear nonthreshold (LNT) model has been used in radiation protection for over 40 years and has been hotly debated. It relies heavily on human epidemiology, with support from radiobiology. The scientific underpinnings include NCRP Report No. 136 ('Evaluation of the Linear-Nonthreshold Dose-Response Model fo...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Boice JD Jr

    更新日期:2017-10-01 00:00:00

  • Cell cycle dependence for the induction of 6-thioguanine-resistant mutations: G2/M stage is distinctively sensitive to 252Cf neutrons but not to 60Co gamma-rays.

    abstract::Cell cycle dependence for the induction of 6-thioguanine-resistant mutation was investigated using synchronized mouse L5178Y cells after exposure to 4 Gy to 60Co gamma-rays or 1 Gy of 252Cf radiation (fission neutrons). Maximal mutation frequency was observed immediately after release from colcemid block (G2/M phase) ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Tauchi H,Nakamura N,Sawada S

    更新日期:1993-04-01 00:00:00

  • Physical and biophysical properties of proton tracks of energies 1 keV to 300 MeV in water.

    abstract:PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Liamsuwan T,Uehara S,Emfietzoglou D,Nikjoo H

    更新日期:2011-02-01 00:00:00

  • Computer evaluation of direct and indirect damage induced by free and DNA-bound iodine-125 in the chromatin fibre.

    abstract:PURPOSE:When Iodine-125 decays within chromatin, several in vivo experiments have shown that the radiobiological effects are caused mainly by indirect mechanisms and that more than one DNA double strand break (DSB) is produced per decay. We present calculations to evaluate the contribution of direct and indirect effect...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Terrissol M,Edel S,Pomplun E

    更新日期:2004-11-01 00:00:00

  • Identification of genes regulated by UV/salicylic acid.

    abstract:PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Paunesku T,Chang-Liu CM,Shearin-Jones P,Watson C,Milton J,Oryhon J,Salbego D,Milosavljevic A,Woloschak GE

    更新日期:2000-02-01 00:00:00

  • WAM to SeeSaw model for cancer therapy - overcoming LQM difficulties.

    abstract:PURPOSE:The assessment of biological effects caused by radiation exposure has been currently carried out with the linear-quadratic (LQ) model as an extension of the linear non-threshold (LNT) model. In this study, we suggest a new mathematical model named as SeaSaw (SS) model, which describes proliferation and cell dea...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Bando M,Tsunoyama Y,Suzuki K,Toki H

    更新日期:2020-12-15 00:00:00

  • Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes.

    abstract::Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently dete...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Schmid E,Zitzelsberger H,Braselmann H,Gray JW,Bauchinger M

    更新日期:1992-12-01 00:00:00

  • Does tirapazamine (SR-4233) have any cytotoxic or sensitizing effect on three human tumour cell lines at clinically relevant partial oxygen pressure?

    abstract::Solid human tumours contain areas with low oxygen tension (pO2). For bioreductive drugs it is important to define the cytotoxic effect according to drug concentration and to clinically relevant pO2. In this study, the pO2 dependence of the survival of three human cell lines (HRT 18, Na11 +, and MEWO), exposed to tirap...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Lartigau E,Guichard M

    更新日期:1995-02-01 00:00:00

  • Endothelial cell proliferation in the rat heart following local heart irradiation.

    abstract::Radiation-induced myocardial degeneration in the rat is preceded by changes in capillary structure and function, which may be a major factor in the pathogenesis of radiation-induced heart disease. In order to investigate the mechanism of capillary damage we studied endothelial cell proliferation in untreated control r...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Lauk S,Trott KR

    更新日期:1990-05-01 00:00:00

  • The influence of glutathione on single-strand breakage in single-stranded DNA irradiated in aqueous solution in the absence and presence of oxygen.

    abstract::The yields of strand break formation (Gssb) in single-stranded DNA (ssDNA) initiated by radiation-generated OH radicals have been determined using the method of low-angle laser light scattering (LALLS). The irradiations were carried out in aqueous, N2O-saturated solutions in the absence and presence of oxygen and at d...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Liphard M,Bothe E,Schulte-Frohlinde D

    更新日期:1990-10-01 00:00:00

  • Involvement of bystander effect in suppression of the cytokine production induced by heavy-ion broad beams.

    abstract:PURPOSE:Immune cells accumulate in and around cancers and cooperate with each other using specific cytokines to attack the cancer cells. The heavy-ion beams for cancer therapy may stimulate immune cells and affect on the immune system. However, it is still poorly understood how the immune cells are stimulated by ion-be...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Mutou-Yoshihara Y,Funayama T,Yokota Y,Kobayashi Y

    更新日期:2012-03-01 00:00:00

  • The effect of the radioprotector WR-2721 and WR-1065 on mitochondrial lipid peroxidation.

    abstract::The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Tretter L,Rónai E,Szabados G,Hermann R,Andó A,Horváth I

    更新日期:1990-03-01 00:00:00

  • Solar radiation induced skin damage: review of protective and preventive options.

    abstract:PURPOSE:Solar energy has a number of short- and long-term detrimental effects on skin that can result in several skin disorders. The aim of this review is to summarise current knowledge on endogenous systems within the skin for protection from solar radiation and present research findings to date, on the exogenous opti...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Svobodová A,Vostálová J

    更新日期:2010-12-01 00:00:00

  • Radioprotection of C3H mice by recombinant human interleukin-1 alpha.

    abstract::High doses of rHIL-1 protected C3H/HeN mice against lethal radiation damage. The optimum time of radioprotection was found when this cytokine was administered at 4 h and at 20 h before irradiation. The dose reduction factors when the IL-1 was administered at those times were about 1.30. The radioprotection at the opti...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Wu SG,Tuboi A,Miyamoto T

    更新日期:1989-10-01 00:00:00

  • Biphasic survival curves for XRS radiosensitive cells: subpopulations or transient expression of repair competence?

    abstract::Four of the most radiosensitive xrs variants of CHO-K1 cells, obtained after mutagenizing treatment with EMS, have been studied in detail over three to five decades of cell survival. Although these lines were initially reported to have very steep exponential survival curves, and to vary in sensitivity between themselv...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Denekamp J,Whitmore GF,Jeggo P

    更新日期:1989-04-01 00:00:00

  • Low folate status increases chromosomal damage by X-ray irradiation.

    abstract:PURPOSE:To examine how folate status influences chromosomal damage following X-ray irradiation. MATERIAL AND METHODS:In an animal study, mice were fed either a low, basal, or high folic acid diet (0, 2, or 40 mg/kg diet, respectively) for 4 weeks, and then given total body irradiation (TBI) at 0.5 Gy. In a human study...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,随机对照试验


    authors: Endoh K,Murakami M,Araki R,Maruyama C,Umegaki K

    更新日期:2006-04-01 00:00:00

  • RBE of α-particles from (211)At for complex DNA damage and cell survival in relation to cell cycle position.

    abstract:PURPOSE:To investigate cell cycle effects and relative biological effectiveness (RBE) of α-particles from the clinically relevant radionuclide Astatine-211 ((211)At), using X-rays as reference radiation. Double-strand breaks (DSB), non-DSB clusters containing oxidised purines and clonogenic survival were investigated. ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Claesson K,Magnander K,Kahu H,Lindegren S,Hultborn R,Elmroth K

    更新日期:2011-04-01 00:00:00

  • Heat effects on the repair of DNA double-strand breaks in CHO cells.

    abstract::The effect of heat (43-45 degrees C) on the induction and the repair of DNA double-strand breaks (dsb) was studied in CHO cells after 60 Gy of X-rays using constant-field gel electrophoresis. Heat given prior to irradiation was found not to alter the number of dsb when measured immediately after irradiation. In non-he...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Dahm-Daphi J,Brammer I,Dikomey E

    更新日期:1997-08-01 00:00:00

  • Repeated 0.5-Gy gamma-ray irradiation attenuates autoimmune manifestations in MRL-lpr/lpr mice.

    abstract:PURPOSE:MRL-lpr/lpr mice, a model for various autoimmune diseases, were repeatedly irradiated with 0.5 Gy of gamma-rays, and changes in their autoimmune manifestations were investigated. MATERIALS AND METHODS:MRL-lpr/lpr mice at 13 weeks of age were maintained in plastic cages and exposed whole-body to 0.5 Gy gamma-ra...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Tanaka T,Tago F,Fang SP,Shimura N,Kojima S

    更新日期:2005-10-01 00:00:00

  • Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry.

    abstract:PURPOSE:The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of thi...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Grégoire E,Roy L,Buard V,Delbos M,Durand V,Martin-Bodiot C,Voisin P,Sorokine-Durm I,Vaurijoux A,Voisin P,Baldeyron C,Barquinero JF

    更新日期:2018-03-01 00:00:00

  • The application of the linear quadratic model to compensate the effects of prolonged fraction delivery time on a Balb/C breast adenocarcinoma tumor: An in vivo study.

    abstract::Purpose To investigate the effect of increasing the overall treatment time as well as delivering the compensating doses on the Balb/c breast adenocarcinoma (4T1) tumor. Materials and methods A total of 72 mice were divided into two aliquots (classes A and B) based on the initial size of their induced tumor. Each class...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Nikzad S,Hashemi B,Hasan ZS,Mozdarani H,Baradaran-Ghahfarokhi M,Amini P

    更新日期:2016-01-01 00:00:00

  • Molecular hydrogen attenuates radiation-induced nucleobase damage to DNA in aerated aqueous solutions.

    abstract:PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Abou-Hamdan M,Gardette B,Cadet J,Gharib B,De Reggi M,Douki T,Triantaphylides C

    更新日期:2016-09-01 00:00:00

  • A review of radiation countermeasures focusing on injury-specific medicinals and regulatory approval status: part III. Countermeasures under early stages of development along with 'standard of care' medicinal and procedures not requiring regulatory approv

    abstract:PURPOSE:Terrorist attacks, with their intent to maximize psychological and economic damage as well as inflicting sickness and death on given targeted populations, are an ever-growing worldwide concern in government and public sectors as they become more frequent, violent, and sensational. If given the chance, it is lik...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章,评审


    authors: Singh VK,Hanlon BK,Santiago PT,Seed TM

    更新日期:2017-09-01 00:00:00

  • Metalloporphyrin antioxidants ameliorate normal tissue radiation damage in rat brain.

    abstract:PURPOSE:We examined the effects of manganese (III) meso-tetrakis (diethyl-2-5-imidazole) porphyrin, a metalloporphyrin antioxidant (MPA), on neural tissue radiation toxicity in vivo and on tumour cell radiosensitivity in vitro. MATERIALS AND METHODS:MPA was administered directly into the right lateral ventricle of you...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Pearlstein RD,Higuchi Y,Moldovan M,Johnson K,Fukuda S,Gridley DS,Crapo JD,Warner DS,Slater JM

    更新日期:2010-02-01 00:00:00

  • Severe mental retardation after large prenatal exposures to bomb radiation. Reduction in oxygen transport to fetal brain: a possible abscopal mechanism.

    abstract::Reasonable expectations, based on the normal physiology of the human fetus, cast new light on the possibilities of extrapolation from the finding of severe mental retardation (SMR) in Japanese bomb survivors after maternal exposure to 1.8-5.5 Gy T65DR Gy. After such large exposures the fetal haematopoietic tissues (DS...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Mole RH

    更新日期:1990-10-01 00:00:00

  • A Monte Carlo calculation of cell inactivation by light ions.

    abstract::This study simulates the exposure of V79 Chinese hamster fibroblasts to low-energy protons, deuterons and alpha-particles in the LET range 10-200 keV/microm. The starting assumption is that the induction of clustered lesions in DNA is a fundamental step for cell inactivation. A non-homogeneous cell population was simu...

    journal_title:International journal of radiation biology

    pub_type: 杂志文章


    authors: Ottolenghi A,Monforti F,Merzagora M

    更新日期:1997-11-01 00:00:00