Abstract:
:This study evaluated the cell-mediated (CMI) and humoral immune responses in four Beagle dogs five to six years after single inhalation exposures to different monodisperse 239PuO2 aerosols (0.72-1.4 microns activity median aerodynamic diameter). These exposures resulted in initial lung burdens ranging from 19 to 35 kBq. Four nonexposed dogs were used as age-matched controls. Anesthetized dogs were immunized by instillation of sheep red blood cells (SRBC) into selected lung lobes. Cells and fluids were obtained serially from blood samples and by bronchoalveolar lavage of the saline- and SRBC-treated lung lobes at 5-20 days after immunization. The CMI response evaluated by the leukocyte procoagulant activity test was similar in the saline- and SRBC-treated lobes of both groups of dogs. The humoral immune response was measured by the enzyme-linked immunosorbent assay. No differences were shown between the amount of antibody measured in the sera or lung lavages from control or Pu-exposed dogs. Histopathology of the tracheobronchial lymph nodes from the Pu-exposed dogs showed them to be fibrotic with no lymphoid cells, suggesting that these tissues could not respond to the antigen deposited in the lungs. However, both mediastinal and sternal lymph nodes did contain lymphoid tissue, and were likely to be the lymphoid tissues that produced the immunity to the antigen deposited in the lungs of the exposed dogs. Although both exposed and control dogs produced immune responses to the antigen instilled into their lungs, differences were observed in the number of neutrophils in lung lavages from the control and exposed animals. There was a dramatic influx of neutrophils into both the saline- and SRBC-treated lung lobes of the Pu-exposed dogs that was not seen in the age-matched controls. This suggests that the inhaled 239PuO2 produced chronically-active inflammation in the lung which may contribute to recruitment of lymphocytes to the lung following intrapulmonary deposition of antigen. In conclusion, the immune responses induced by lung immunization of dogs that had inhaled 239PuO2 were not suppressed by large doses of chronic alpha irradiation of the lungs and tracheobronchial lymph nodes, indicating that local pulmonary immune responses are preserved despite severe radiation-induced alteration of these tissues.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Galvin JB,Bice DE,Guilmette RA,Muggenburg BA,Haley PJdoi
10.1080/09553008914550311subject
Has Abstractpub_date
1989-02-01 00:00:00pages
285-96issue
2eissn
0955-3002issn
1362-3095pii
TYDAFD04CGQK93NGjournal_volume
55pub_type
杂志文章abstract::Bacterial infection of simple wounds was studied directly and quantitatively in adult mice given 6.5 Gy 60Co. Three days later, when neutropenia was evident, the skin and the medial gluteus muscle of anaesthetized mice were incised. A suspension of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae or Stre...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551671
更新日期:1990-08-01 00:00:00
abstract::Purpose: This review discusses recent developments in our understanding of biological and physiological mechanisms underlying radiation cataractogenesis. The areas discussed include effects of low-dose exposures to the lens including potential relevance of non-targeted effects, the development of new personal-protecti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1767820
更新日期:2020-05-26 00:00:00
abstract::237Np nitrate was injected intravenously into 4-week-old (young) and 10-12-week-old (adult) female albino Sprague-Dawley rats. The amounts given were 52 (young), 5.2 (adult) and 26 kBq kg-1 body weight (adult). The microscopic distribution in the femur and the lumbar vertebrae was studied. Initially, neptunium was dis...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550511
更新日期:1993-03-01 00:00:00
abstract:PURPOSE:Melanoma is mainly treated by surgery and rarely with radiation because of the high radioresistance of this tumor. Nevertheless, radiotherapy is the preferred treatment modality for unresectable lesions and avoiding cosmetic disfigurement caused by surgical excision. This study investigated the therapeutic adva...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1276309
更新日期:2017-05-01 00:00:00
abstract:PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000701570196
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:The low dose (<1 Gy) survival curve of mammalian cells is characterized by a region of hypersensitivity (HRS) followed by increased resistance (IRR). Above 1 Gy, the survival response can be described with a smooth downward-bending curve. Indirect evidence has indicated that the IRR response might reflect an in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138646
更新日期:2000-03-01 00:00:00
abstract::Purpose: A study is presented of the irradiation of cancerous cervical cell line HeLa loaded with a platinum salt, betamethasone and deoxyglucose. The presence of the platinum increases the free-radical concentration and augments the cell death rate, whereas betamethasone or deoxyglucose induces radiosensitization by ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1721594
更新日期:2020-05-01 00:00:00
abstract::The effect of a siderophore analogue 3,4,3-LIHOPO has been investigated in rat after intramuscular injection of 238Pu, 239Pu and 241Am simulating puncture wounds. Various treatment regimens were used to remove the radioactivity from its injection site and to reduce its retention in body tissues. The local deposits cou...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145382
更新日期:1996-07-01 00:00:00
abstract::Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, a multiscale high-throughput multi-omics analysis was applied with a protocol for chronic exposure to the rat kidney. Methods: Male and female rats were contaminated for nine months through their drinking water with a nont...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2019.1577567
更新日期:2019-06-01 00:00:00
abstract::A comparison has been made of the in vitro light-activated drug cytotoxicities of two different porphyrin compounds, Photofrin II and TPPS4. An early passage human colon adenocarcinoma cell line, WiDr, has been exposed to either drug for 24 h, the excess drug washed from the cells and the cells irradiated with light u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552061
更新日期:1988-10-01 00:00:00
abstract:PURPOSE:To propose an improved version of RADACK, a stochastic simulation of radiolytic attack on DNA, that takes into account the reactivity of each amino acid of a specifically bound protein with hydroxyl radicals. To apply it to the natural lactose operator-repressor complex taking advantage of recently reported str...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/0955300031000137788
更新日期:2003-06-01 00:00:00
abstract::Survival of HT29 cells was measured after irradiation with single doses of X-rays (0.05-5 Gy) and neutrons (0.025-1.5 Gy), using a Dynamic Microscopic Imaging Processing Scanner (DMIPS) with which individual cells can be accurately located in tissue culture flasks, their positions recorded, and after an appropriate in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314450831
更新日期:1993-05-01 00:00:00
abstract::Swiss albino mice (7-8 weeks old) were irradiated with different doses (0-25 Gy) of gamma-radiation at a dose-rate of 0.05 Gy/s. The specific activities of glyoxalase I (GI) and glyoxalase II (GII) were determined in the spleen and liver immediately and on the 3rd and 6th day postirradiation. The results indicate that...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314550301
更新日期:1993-02-01 00:00:00
abstract:PURPOSE:To understand the mechanisms of life-shortening due to early neoplastic death caused by chronic low dose-rate (LDR; 20 mGy/22 h/day) radiation which accumulates to a high dose (HD; 8 Gy) (LDR/HD) as reported previously. MATERIALS AND METHODS:Female B6C3F(1) mice were continuously exposed to LDR/HD gamma-rays u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.545861
更新日期:2011-07-01 00:00:00
abstract:PURPOSE:The long-term effect of low and moderate doses of ionizing radiation on the lens is still a matter of debate and needs to be evaluated in more detail. MATERIAL AND METHODS:We conducted a detailed histological analysis of eyes from B6C3F1 mice cohorts after acute gamma irradiation (60Co source; 0.063 Gy/min) at...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2021.1876951
更新日期:2021-01-19 00:00:00
abstract::The relative contributions of the OH-mediated and direct radiation effect on the induction of DNA double-strand breaks (dsbs) were evaluated in two haploid yeast cell lines (GSH+ and gsh-) irradiated under oxic or hypoxic conditions in the absence or presence of 6 M glycerol as an OH radical scavenger. Gsh- cells are ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551721
更新日期:1993-11-01 00:00:00
abstract::One of the important temporal stages of radiation action in cellular systems is the chemical phase, where oxygen fixation reactions compete with chemical repair reactions involving reducing agents such as GSH. Using the gas explosion technique it is possible to follow the kinetics of these fast (greater than 1 ms) rea...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551571
更新日期:1992-06-01 00:00:00
abstract:PURPOSE:To honour perhaps the most influential man in the radiation sciences, Henry S. Kaplan, by reviewing the field of tumour hypoxia, one of his many interests. RESULTS:It was postulated over 50 years ago by Thomlinson and Gray that human solid tumours would contain hypoxic cells and that they would exert a negativ...
journal_title:International journal of radiation biology
pub_type: 历史文章
doi:10.3109/09553002.2010.492492
更新日期:2010-11-01 00:00:00
abstract:PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802499212
更新日期:2008-11-01 00:00:00
abstract::Free radical formation in aqueous solutions of ubiquinone (coenzyme Qo) induced by ultrasound was studied by EPR and spin trapping with 3,5-dibromo-4-nitrosobenzene sulphonate. When aqueous solutions of ubiquinone were sonicated with 50-kHz ultrasound in the presence of argon or nitrogen, radicals formed by addition o...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096146246
更新日期:1996-01-01 00:00:00
abstract:PURPOSE:The LET position of the RBE maximum and its dependence on the cellular repair capacity was determined for carbon ions. Hamster cell lines of differing repair capacity were irradiated with monoenergetic carbon ions. RBE values for cell inactivation at different survival levels were determined and the differences...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139232
更新日期:1999-11-01 00:00:00
abstract:PURPOSE:The recent rapid increase of hadron therapy applications requires the development of high performance, reliable in vivo models for preclinical research on the biological effects of high linear energy transfer (LET) particle radiation. AIM:The aim of this paper was to test the relative biological effectiveness ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2018.1511928
更新日期:2018-11-01 00:00:00
abstract::CHEF-electrophoresis was used as a technique to detect radiation-induced DNA breakage with special emphasis to biological relevant X-ray doses (0-10 Gy). Fluorescence detection of DNA-fragments using a sensitive image analysis system was directly compared with conventional scintillation counting of 3H-thymidine prelab...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551361
更新日期:1993-08-01 00:00:00
abstract:PURPOSE:Previous work from the authors' group and others has demonstrated that some of the effects of UV irradiation on gene expression are modulated in response to the addition of salicylic acid to irradiated cells. The presumed effector molecule responsible for this modulation is NF-kappaB. In the experiments describ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138844
更新日期:2000-02-01 00:00:00
abstract::Genetic analysis was performed on 72 Italian A-T families ascertained through 91 probands. The frequency of the A-T gene was estimated through Dahlberg's formula, which uses the frequencies of first cousin marriages among the patients' parents and in the general population were obtained from the collection of Catholic...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract:PURPOSE:This review examines the evidence for the hypothesis that epigenetics are involved in the initiation and perpetuation of radiation-induced genomic instability (RIGI). CONCLUSION:In addition to the extensively studied targeted effects of radiation, it is now apparent that non-targeted delayed effects such as RI...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2010.522686
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:To assess intestinal radioprotection by systemic or lumenal tirilazad mesylate and to test potential tumour radioprotection. MATERIALS AND METHODS:Segments of the mid small bowel were surgically exteriorized. Tirilazad was given either intravenously or intralumenally 30 min prior to 11 Gy of X-irradiation to t...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098142617
更新日期:1998-02-01 00:00:00
abstract:PURPOSE:MRL-lpr/lpr mice, a model for various autoimmune diseases, were repeatedly irradiated with 0.5 Gy of gamma-rays, and changes in their autoimmune manifestations were investigated. MATERIALS AND METHODS:MRL-lpr/lpr mice at 13 weeks of age were maintained in plastic cages and exposed whole-body to 0.5 Gy gamma-ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000500519790
更新日期:2005-10-01 00:00:00
abstract::Studies of protein phosphorylation in the second cell cycle of mouse embryos were assessed by [32P]-incorporation and one- and two-dimensional electrophoresis. Unirradiated and X-irradiated embryos were compared. X-irradiation in the G2 phase led to a block in this cell cycle phase. The phosphorylation of proteins wit...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552351
更新日期:1991-09-01 00:00:00
abstract::This study was designed to compare radiosensitization by the oral platinum compound JM216 with cisplatin. RIF1 mouse tumour cells were treated at various doses and at various exposure times with JM216 and irradiated 15 min before the end of drug exposure. The fraction of cells surviving treatment was assessed by colon...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097142834
更新日期:1997-12-01 00:00:00