Abstract:
:We characterized in vitro the effects of gamma-radiation (12.5-100 cGy) on pulmonary microvascular endothelial cell (PMEC) morphology and F-actin organization. Cellular retraction was documented by phase-contrast microscopy and the organization of actin microfilaments was determined by immunofluorescence. Characterization included radiation dose effects, their temporal duration and reversibility of the effects. A dose-dependent relationship between the level of exposure (12.5-100 cGy) and the rate and extent of endothelial retraction was observed. Moreover, analysis of radiation-induced depolymerization of F-actin microfilament stress fibres correlated positively with the changes in PMEC morphology. The depolymerization of the stress fibre bundles was dependent on radiation dose and time. Cells recovered from exposure to reform contact inhibited monolayers > or = 24 h post-irradiation. Concomitantly, the depolymerized microfilaments reorganized to their preirradiated state as microfilament stress fibres arrayed parallel to the boundaries of adjacent contact-inhibited cells. The data presented here are representative of a series of studies designed to characterize low-dose radiation effects on pulmonary microvascular endothelium. Our data suggest that post-irradiation lung injuries (e.g. oedema) may be induced with only a single fraction of therapeutic radiation, and thus microscopic oedema may initiate prior to the lethal effects of radiation on the microvascular endothelium, and much earlier than would be suggested by the time course for clinically-detectable oedema.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Kantak SS,Diglio CA,Onoda JMdoi
10.1080/09553009314551471subject
Has Abstractpub_date
1993-09-01 00:00:00pages
319-28issue
3eissn
0955-3002issn
1362-3095pii
3X34VDPT5E9H90Y9journal_volume
64pub_type
杂志文章abstract:PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138745
更新日期:2000-03-01 00:00:00
abstract:PURPOSE:A meta-analysis was conducted to obtain a 'quantitative' estimate of the extent of genetic damage in mammalian somatic cells exposed to non-ionizing radiation emitted from extremely low frequency electro-magnetic fields (ELF-EMF) and to compare with that in unexposed control cells. METHODS:The methods used for...
journal_title:International journal of radiation biology
pub_type: 杂志文章,meta分析
doi:10.1080/09553000902748575
更新日期:2009-03-01 00:00:00
abstract::Bacterial infection of simple wounds was studied directly and quantitatively in adult mice given 6.5 Gy 60Co. Three days later, when neutropenia was evident, the skin and the medial gluteus muscle of anaesthetized mice were incised. A suspension of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae or Stre...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551671
更新日期:1990-08-01 00:00:00
abstract::The effect of a siderophore analogue 3,4,3-LIHOPO has been investigated in rat after intramuscular injection of 238Pu, 239Pu and 241Am simulating puncture wounds. Various treatment regimens were used to remove the radioactivity from its injection site and to reduce its retention in body tissues. The local deposits cou...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096145382
更新日期:1996-07-01 00:00:00
abstract:PURPOSE:Ionizing radiation causes various types of DNA damage e.g. single strand breaks (SSB) and double strand breaks (DSB), whereby the SSB/DSB ratio is shifted toward the DSB with increasing LET. For the DNA-incorporated Auger electron emitter Iodine-125 a SSB/DSB ratio of 5.4:1 is calculated based on computer simul...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1851059
更新日期:2020-12-03 00:00:00
abstract:PURPOSE:Solar energy has a number of short- and long-term detrimental effects on skin that can result in several skin disorders. The aim of this review is to summarise current knowledge on endogenous systems within the skin for protection from solar radiation and present research findings to date, on the exogenous opti...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.3109/09553002.2010.501842
更新日期:2010-12-01 00:00:00
abstract::Cell killing and the production of DNA double-strand breaks (dsbs) were measured in parallel for V79 cells and a human tumour cell line (Caski) following exposure to radiations of differing linear energy transfer (LET) including accelerated heavy ions. Dsb induction was assessed by neutral filter elution (pH 7.4) and ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551261
更新日期:1993-08-01 00:00:00
abstract::A radionuclide release assay for measuring the in vitro kinetics of cell death has been developed. CHO cells were labelled for 24 h with 3.0 hBq/ml of [125I] iododeoxyuridine (125IUdR) and the fate of the labelled cells and their progeny was monitored at daily intervals by measuring the rate of 125I release. Prelabell...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550361
更新日期:1991-02-01 00:00:00
abstract::Rats were chronically exposed to tritiated water (3HHO) and several tritiated organic compounds [( 3H]leucine, [3H]lysine, [3H]glucose, [3H]glucosamine, [3H]thymidine and [3H]uridine) dissolved in their drinking water. An analysis of tritium in wet and dry tissues of rats at the end of 22 days' chronic exposure showed...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114550731
更新日期:1991-03-01 00:00:00
abstract:PURPOSE:To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data. MATERIALS AND METHODS:Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic exam...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141870
更新日期:1998-06-01 00:00:00
abstract::Experimental data for acute radiation-induced skin reactions are reviewed. These show that for dose fractionation schedules with gaps, repopulation is initiated after a lag period. After this lag period, the isoeffective dose for a given level of skin reaction first increases rapidly, but then slows. The timing of the...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000310001600907
更新日期:2003-07-01 00:00:00
abstract::A novel 2-nitroimidazole with a theophylline side-chain, 7-(4'-(2-nitroimidazol-1-yl)-butyl)-theophylline, (NITP) was as efficient a hypoxic-cell radiosensitizer as misonidazole. However, if cells were irradiated with NITP under hypoxic conditions and then exposed to the drug under aerobic conditions, a much larger ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551671
更新日期:1992-06-01 00:00:00
abstract:PURPOSE:To study by fluorescence in situ hybridization (FISH) the involvement of Chinese hamster chromosomes 3, 4, 8, and 9, and their separate chromosome arms in X-ray-induced aberrations. MATERIALS AND METHODS:Male embryonic primary cells of Chinese hamster were used and metaphases were collected and scored at 20 h ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139700
更新日期:1999-08-01 00:00:00
abstract:PURPOSE:To investigate the mechanism of NFkappaB activation by X-rays in normal primary rat astrocytes. MATERIALS AND METHODS:Primary cultures of type I astrocytes generated from the cortex of neonatal rats were exposed to X-rays with and without various kinase inhibitors and a protease inhibitor. The nuclear or cytop...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141195
更新日期:1998-11-01 00:00:00
abstract:PURPOSE:To investigate the hypothesis that the developmental phase at which an individual encounters radiation damage affects its long-term sensitivity to a subsequent tumourigenic insult. MATERIALS AND METHODS:Either the pregnant C57B16 mouse was exposed to 137Cs gamma-rays at 4 or 15 days post-conception (embryonic ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000139032
更新日期:2000-01-01 00:00:00
abstract::The relative contributions of the OH-mediated and direct radiation effect on the induction of DNA double-strand breaks (dsbs) were evaluated in two haploid yeast cell lines (GSH+ and gsh-) irradiated under oxic or hypoxic conditions in the absence or presence of 6 M glycerol as an OH radical scavenger. Gsh- cells are ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551721
更新日期:1993-11-01 00:00:00
abstract::Pre-implantation embryos of the mouse were studied for the occurrence of an adaptive response, i.e. induction of radio-resistance by a previous low dose. Various experimental designs were checked (initial doses between 3 and 10 cGy; second dose 2-6 Gy at 6-24 h after the first dose). Some of the experiments were carri...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551981
更新日期:1992-08-01 00:00:00
abstract::Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2016.1137105
更新日期:2016-01-01 00:00:00
abstract::Only a small percentage of the DNA damage set by ionizing radiation in the living cell manifests itself as lethal. It is now increasingly accepted that clustered lesions may constitute the kind of damage that the repair enzymes cannot adequately deal with. The question is raised as to whether damage amplification reac...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414551501
更新日期:1994-11-01 00:00:00
abstract:PURPOSE:To investigate cell cycle effects and relative biological effectiveness (RBE) of α-particles from the clinically relevant radionuclide Astatine-211 ((211)At), using X-rays as reference radiation. Double-strand breaks (DSB), non-DSB clusters containing oxidised purines and clonogenic survival were investigated. ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.538127
更新日期:2011-04-01 00:00:00
abstract:PURPOSE:This study is part of a general search for the anatomical bases of the severe mental retardation syndrome caused by prenatal irradiation. More specifically, it seeks reasons for the high radiosensitivity of a white matter area, the cingulum of the corpus callosum. MATERIALS AND METHODS:Pregnant primiparous Wis...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530099139494
更新日期:1999-10-01 00:00:00
abstract::Radioiodine ((131)I) induced a dose- and time-dependent increase in DNA single-strand breaks (DNA-ssb) in human (G0) mononuclear blood cells (MNC) in vitro. Incubation of MNC with 22MBq (131)I/ml at 4 degrees C caused a linear, time-dependent induction of DNA-ssb (increase in elution rate: 24.7 x 10(-3) h(-1) per 100 ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143103
更新日期:1997-11-01 00:00:00
abstract:PURPOSE:Recently, it was found that MK615 possessed an anti-proliferative ability on treated cancer cells as a consequence of triterpenoid compounds. It is well known that radiation affects cellular-mediated immunity in cancer patients who are treated with radiotherapy. Similarly, the ability of triterpenoid compounds ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518202
更新日期:2011-01-01 00:00:00
abstract::Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately t...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553008914551971
更新日期:1989-11-01 00:00:00
abstract:PURPOSE:It has been previously argued that the use of the one-electron oxidants (SCN)2(*-) and Br2(*-) with plasmid DNA leads to the formation of DNA guanyl radicals. These guanyl radical species are intermediates in the DNA damage produced by processes such as photo-ionization and ionizing irradiation. The present pap...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110069119
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:The prolonged delivery times associated with intensity modulated radiation therapy (IMRT) may reduce treatment effectiveness of radiation therapy for cancers with short repair half-times. In this study, in vitro radiation experiments with DU-145 prostate cancer cells were designed to quantify the half-time of s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802061285
更新日期:2008-06-01 00:00:00
abstract:PURPOSE:Animal models that accurately reflect human responses to radiation injury are needed for advanced mechanistic investigation and development of effective therapeutics. The rabbit is an established animal model accepted by the FDA for studies of cardiovascular disease, lipid metabolism, the development of anticoa...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1820606
更新日期:2020-10-26 00:00:00
abstract::Radiation-induced myocardial degeneration in the rat is preceded by changes in capillary structure and function, which may be a major factor in the pathogenesis of radiation-induced heart disease. In order to investigate the mechanism of capillary damage we studied endothelial cell proliferation in untreated control r...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009014551131
更新日期:1990-05-01 00:00:00
abstract::The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097143590
更新日期:1997-07-01 00:00:00
abstract::The effect of local stimulation on mitotic activity and radiation tolerance was studied in mouse tongue mucosa. Silver nitrate solution (0.5-20%) was used for local conditioning. The most effective protocol comprised three daily treatments (days 0-2), yielding a delayed increase in 24 h mitotic counts by about 30% on ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214551061
更新日期:1992-03-01 00:00:00