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Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines.

Abstract：

:We used the 'comet assay' to compare the amount of radiation-induced DNA damage in three tumour cell lines (MeWo, PECA 4451 and PECA 4197) and the extent of DNA repair in two of these lines (MeWo and PECA 4197). Tumour cells were irradiated with X-rays (0.1-10 Gy), embedded in agarose on slides, lysed with sodium dodecyl sulphate and exposed to an electric field. DNA migrated within the agarose and formed comets whose length depended on the amount of DNA damage. When the cells were incubated at 37 degrees C for various time intervals before electrophoresis started, the comets shrank in the course of time, indicating repair of DNA damage. All three cell lines showed the same extent of DNA damage after radiation exposure, despite the fact that in the colony-forming assay MeWo and PECA 4451 were much more sensitive to radiation exposure than PECA 4197. The repair characteristics, however, were markedly different for MeWo and PECA 4197 cells. PECA 4197 cells showed a much faster restoration of the original shape of the cell nucleus than MeWo cells.

journal_name

Int J Radiat Biol

journal_title

International journal of radiation biology

authors

Müller WU,Bauch T,Streffer C,Niedereichholz F,Böcker W

doi

10.1080/09553009414550371

subject

Has Abstract

pub_date

1994-03-01 00:00:00

pages

315-9

issue

3

eissn

0955-3002

issn

1362-3095

pii

V8LC1CUVNTMYK7DY

journal_volume

65

pub_type

杂志文章

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