Abstract：
PURPOSE:To determine whether murine lymphoid cell lines can discriminate between high- and low-LET (linear energy transfer) radiation-induced DNA lesions. MATERIALS AND METHODS:Sensitivity to killing by DNA-incorporated 3H and 125I decays, accumulated during storage in the gas phase of a liquid nitrogen tank, was determined by clonogenic survival assay. RESULTS:Induction of a lethal event in the STRij-4-2.2, WEHI-22.1, and L5178Y-R cell lines required approximately 30 times more 3H than 125I decays. Hence, the same ratio of 3H to 125I decays was found irrespective of whether the cell lines contained mutant or wild-type p53 and irrespective of whether they underwent rapid interphase or mitosis-related apoptosis after irradiation. The 18-81 cell line differed in showing a ratio of around 21 and it is argued that this may be a consequence of v-ABL over-expression. The assumption that DNA-incorporated 3H and 125I decays are low- and high-LET-like events respectively was confirmed by the similar sensitivity of L5178Y-R and -S cells to killing by 125I decays in contrast with their difference in sensitivity to 3H decays. CONCLUSIONS:The difference in lethal effectiveness between DNA-incorporated 3H and 125I decays can be explained by the hypothesis that simple DSB (double-strand breaks) are non-lethal and that cell killing is attributable to complex DSB. The low-LET radiation-specific sensitization of L5178Y-S cells may reflect defective repair of a DNA lesion class (presumably simple DSB) that is differentially induced by high- and low-LET radiation and is non-lethal to cells with normal repair capacity.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Radford IRdoi
10.1080/09553000110120337subject
Has Abstractpub_date
2002-06-01 00:00:00pages
457-66issue
6eissn
0955-3002issn
1362-3095journal_volume
78pub_type
杂志文章abstract：:The recently released data on cancer incidence in the Japanese atomic bomb survivors are analysed using a variety of relative risk models which take account of errors in estimates of dose to assess the dose-response at low doses. For all solid cancers analysed together there is a significant positive dose-response (at...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145364
更新日期：1996-07-01 00:00:00
abstract：PURPOSE:To estimate gamma-ray doses received by Hiroshima atomic bomb survivors using electron spin resonance (ESR) of tooth enamel and to compare the results with cytogenetic data. MATERIALS AND METHODS:Tooth enamel ESR was performed for 100 teeth donated from 69 Hiroshima survivors, and conventional cytogenetic exam...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530098141870
更新日期：1998-06-01 00:00:00
abstract：:Pulsed-field electrophoresis is being used extensively in the gene mapping studies and in the analysis of DNA strand breakage by ionizing radiation. We have evaluated the relationship between the fraction of S phase DNA in a cell population and its ability to modify the migration of DNA in pulsed-field gel electrophor...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096145427
更新日期：1996-06-01 00:00:00
abstract：PURPOSE:The present work summarises the history and current status of research into the importance of cancer stem cells for radiobiological research and for clinical radiation oncology. An effort is made to differentiate clonogenicity from stemness of cancer cells. CONCLUSION:In radiooncology, cancer stem cells have b...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000902836404
更新日期：2009-05-01 00:00:00
abstract：:Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009214552451
更新日期：1992-11-01 00:00:00
abstract：PURPOSE:To study cell damage and possible apoptosis in K562 human erythroleukemic cells exposed for 2 h to an extremely low frequency (ELF) 50 Hz sinusoidal magnetic field with a magnetic induction of either 1 or 5 mT using high resolution proton nuclear magnetic resonance (1H-NMR) spectroscopy. MATERIALS AND METHODS:...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000500102985
更新日期：2005-02-01 00:00:00
abstract：PURPOSE:To illustrate the progress in normal tissue radiation biology over the last five decades and its impact on radiotherapy. MATERIALS AND METHODS:Major milestones over the last 50 years and their consequences for radiation oncology are described: The identification of clonogenic cell survival and the (target) ste...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553000902985136
更新日期：2009-07-01 00:00:00
abstract：PURPOSE:The radiation-induced bystander effect (RIBE) has important implications for the efficiency of radiotherapy but the underlying role of cellular metabolism is widely unknown. The roles of synthesis of cytochrome c oxidase 2 (SCO2), a key effector for respiratory chain, and related signaling factors in α-particle...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.817706
更新日期：2013-12-01 00:00:00
abstract：PURPOSE:The main aim of the present study is to gain mechanistic insights into the modulating effect of molecular hydrogen on the γ-radiation-induced alteration pathways of DNA nucleobases. MATERIALS AND METHODS:Aerated aqueous solutions of calf thymus DNA were exposed to a (60)Co source at doses ranging from 0 to 55 ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2016.1206234
更新日期：2016-09-01 00:00:00
abstract：PURPOSE:Immune cells accumulate in and around cancers and cooperate with each other using specific cytokines to attack the cancer cells. The heavy-ion beams for cancer therapy may stimulate immune cells and affect on the immune system. However, it is still poorly understood how the immune cells are stimulated by ion-be...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2012.636138
更新日期：2012-03-01 00:00:00
abstract：:Cell death induced by various radiation doses in Molt-4 and L5178Y cells was studied. Molt-4 cells have a low threshold for induction of interphase death while L5178Y cells have a high threshold. Interphase death in Molt-4 cells at doses > 100 Gy and in L5178Y cells at doses > 300 Gy showed typical necrosis. In contra...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009314551101
更新日期：1993-07-01 00:00:00
abstract：:Context: Some individuals are reporting being sensitive to electromagnetic fields. They report some unspecific symptoms like headeache, sleep disruption, heart palpitations, itching, etc. The lack of scientific objective links between EHS symptoms and EMF exposure renders the diagnosis of EHS more complex. Purpose: Ou...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1569776
更新日期：2019-06-01 00:00:00
abstract：PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553000701570196
更新日期：2007-11-01 00:00:00
abstract：:An iodinated DNA ligand, iodo Hoechst 33258, which binds in the minor groove of DNA, enhances DNA strand breakage and cell killing by UV-A irradiation. The sites of UV-induced strand breaks reflect the known sequence specificity of the ligand. ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014551061
更新日期：1990-05-01 00:00:00
abstract：:Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2016.1137105
更新日期：2016-01-01 00:00:00
abstract：PURPOSE:This review is a contribution to the memory of Dr William (Bill) Morgan and highlights an area of research and deliberation that he considered extremely important in support of the setting of protective radiation dose limits. Biological research has generally played a minor role in the estimation of adverse hea...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2017.1290848
更新日期：2017-10-01 00:00:00
abstract：PURPOSE:We aimed for a quantitative evaluation that justifies guidelines for evacuation which take into consideration both the human and economic costs. To the best of our knowledge, such an evaluation has not been performed yet. The present guidelines published by the International Atomic Energy Agency (IAEA) are prob...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2020.1779962
更新日期：2020-11-01 00:00:00
abstract：PURPOSE:The assessment of biological effects caused by radiation exposure has been currently carried out with the linear-quadratic (LQ) model as an extension of the linear non-threshold (LNT) model. In this study, we suggest a new mathematical model named as SeaSaw (SS) model, which describes proliferation and cell dea...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2021.1854487
更新日期：2020-12-15 00:00:00
abstract：:Ruthenium-106 in citrate solution was administered intravenously to rat at different stages of pregnancy and to guinea-pig either before conception or in late pregnancy. The results for rat showed that retention in the embryo/foetus measured at 3-5 days after administration increased from about 0.0002% of injected act...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
abstract：:The mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological do...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009414551321
更新日期：1994-10-01 00:00:00
abstract：PURPOSE:The purpose of this study was to assess the effect of a lavage solution containing methoxypolyethylene glycol 4-(3,4-dihydroxyphenethylamino)-4-oxobutanoate (MDO) for whole lung lavage (WLL) in dogs after the inhalation of depleted uranium (DU) dust at a dose of 30 mgUkg-1. MATERIALS AND METHODS:WLL was perfor...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2018.1446228
更新日期：2018-05-01 00:00:00
abstract：PURPOSE:The effect of extremely low frequency electromagnetic fields (ELF-EMF) on bacteria has attracted attention due to its potential for beneficial uses. This research aimed to determine the effect of ELF-EMF on bacterial membrane namely the membrane potential, surface potential, hydrophobicity, respiratory activity...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2015.1101500
更新日期：2016-01-01 00:00:00
abstract：:The effects of UV radiation on humans and animals are receiving increasing attention and much interest has recently been focused on the environmental effects of UV A and UV B. This study compares the in vitro effects of UV A and UV B on the clonogenic survival of two human skin keratinocyte cell lines, HaCaT which are...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143590
更新日期：1997-07-01 00:00:00
abstract：:Radioiodine ((131)I) induced a dose- and time-dependent increase in DNA single-strand breaks (DNA-ssb) in human (G0) mononuclear blood cells (MNC) in vitro. Incubation of MNC with 22MBq (131)I/ml at 4 degrees C caused a linear, time-dependent induction of DNA-ssb (increase in elution rate: 24.7 x 10(-3) h(-1) per 100 ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530097143103
更新日期：1997-11-01 00:00:00
abstract：PURPOSE:One of the most important issues regarding radiofrequency electromagnetic fields (RF-EMF) is their effect on genetic material. Therefore, we investigated the cytogenotoxic effects of 900 MHz radiofrequency electromagnetic fields (RF-EMF) and the effect of a recovery period after exposure to RF-EMF on bone marro...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.3109/09553002.2013.809170
更新日期：2013-11-01 00:00:00
abstract：:Two novel nitroimidazole-carboranes were examined for their uptake and retention in two experimental murine solid tumours and in some normal tissues, using in vivo 11B magnetic resonance spectroscopy. The compounds were injected i.p. at 0.8mmol/kg into mice bearing either the SCCVII/Ha squamous cell carcinoma or KHT s...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530096144798
更新日期：1996-11-01 00:00:00
abstract：:A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title：International journal of radiation biology
pub_type： 杂志文章,评审
doi：10.1080/09553002.2018.1454616
更新日期：2019-01-01 00:00:00
abstract：:Objective: To quantify DNA damage in patients undergoing non-contrast and contrast-enhanced 18F-FDG PET/CT whole body positron emission tomography/computed tomography (WB PET/CT) investigations using comet assay technique and micronucleus assay, and to study the effect of other baseline parameters of patients on DNA d...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553002.2019.1577569
更新日期：2019-06-01 00:00:00
abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/09553009014552611
更新日期：1990-03-01 00:00:00
abstract：PURPOSE:To obtain compounds that will effectively reduce the fixation of uranium in its main target organs: bone and kidney. There is an urgent need for a chelating agent that is suitable and available for human use. MATERIALS AND METHODS:The efficacy of ethane-1-hydroxy-1,1-bisphosphonate (EHBP), already in use as a ...
journal_title：International journal of radiation biology
pub_type： 杂志文章
doi：10.1080/095530099139331
更新日期：1999-11-01 00:00:00
